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SOX2 antibody - 347 003C3

SOX proteins are transcription factors involved in CNS development
Rabbit polyclonal purified antibody
Cat. No.: 347 003C3
Amount: 50 µg
Price: $465.00
Cat. No. 347 003C3 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Cyanine 3.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Label Sulfo-Cyanine 3
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse SOX2 (UniProt Id: P48432)
Reactivity Reacts with: rat (D4A543), mouse (P48432), human (P48431).
Other species not tested yet.
Data sheet 347_003c3.pdf
Cat. No.: 347 003C3
Amount: 50 µg
Price: $465.00
Background

Sex determining region of Y chromosome (Sry)-related high mobility group box1-3 or SOX 1-3 proteins belong to the earliest transcription factors expressed in the developing CNS
SOX1, SOX2 and SOX3 constitute the B1-subgroup of the Sox gene family 12. They are expressed by most progenitor cells of the developing CNS and are generally downregulated by neural cells when they exit the cell cycle and differentiate.