SOX2 antibody - 347 003

SOX proteins are transcription factors involved in CNS development

Rabbit polyclonal purified antibody

Cat. No.: 347 003

Amount: 50 µg

Price: $375.00

Cat. No. 347 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet (AP staining) IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse SOX2 (UniProt Id: P48432)
Reactivity	Reacts with: rat (D4A543), mouse (P48432), human (P48431). Other species not tested yet.
Data sheet	347_003.pdf

References for SOX2 - 347 003

Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.
Gunaseelan S, Wang Z, Tong VKJ, Ming SWS, Razar RBBA, Srimasorn S, Ong WY, Lim KL, Chua JJE
Human molecular genetics (2021) : . 347 003 ICC; tested species: human

Cat. No.: 347 003

Amount: 50 µg

Price: $375.00

ICC

IHC

IHC-P

Background

Sex determining region of Y chromosome (Sry)-related high mobility group box1-3 or SOX 1-3 proteins belong to the earliest transcription factors expressed in the developing CNS
SOX1, SOX2 and SOX3 constitute the B1-subgroup of the Sox gene family 12. They are expressed by most progenitor cells of the developing CNS and are generally downregulated by neural cells when they exit the cell cycle and differentiate.

1 ) Poh YC et al., Nat Commun (2014)
2 ) Thiel G et al., Biochem. J. (2013)
3 ) Archer TC et al., Dev. Biol. (2011)
4 ) Kan L et al., Dev. Biol. (2004)
5 ) Bylund M et al., Nat. Neurosci. (2003)
6 ) Bani-Yaghoub M et al., Dev. Biol. (2006)
7 ) Graham V et al., Neuron (2003)
8 ) Wood HB et al., Mech. Dev. (1999)
9 ) Pevny LH et al., Development (1998)
10 ) Stevanović M et al., Hum. Mol. Genet. (1993)

Protocols

ICC

IHC

IHC-P

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