Synaptic Systems

Cat. No.: 344 002

Amount: 200 µl

Price: $355.00

Cat. No. 344 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 226 from rat GAP43 (UniProt Id: P07936)
Reactivity	Reacts with: rat (P07936), mouse (P06837). Other species not tested yet.
Data sheet	344_002.pdf

References for GAP43 - 344 002

Ketone bodies promote stroke recovery via GAT-1-dependent cortical network remodeling.
Lin YH, Yang D, Ni HY, Xu XM, Wu F, Lin L, Chen J, Sun YY, Huang ZQ, Li SY, Jiang PL, et al.
Cell reports (2023) 424: 112294. 344 002 WB; tested species: mouse

HDAC2 (Histone deacetylase 2): a critical factor in environmental enrichment-mediated stroke recovery.
Lin YH, Yao MC, Wu HY, Dong J, Ni HY, Kou XL, Chang L, Luo CX, Zhu DY
Journal of neurochemistry (2020) : . 344 002 WB; tested species: mouse

Cat. No.: 344 002

Amount: 200 µl

Price: $355.00

ICC

IHC

Background

The neuronal growth-associated protein GAP 43 plays an important role in nerve growth and is a major component of the motile "growth cones" that form the tips of elongating axons. It is the major substrate for protein kinase C and is associated with axonal and dendritic filopodia induction. GAP 43 is also known as Neuromodulin, B-50, P-57, pp46, and F1.

1 ) Gnanapavan S et al., J. Neuroimmunol. (2014)
2 ) Donnelly CJ et al., J. Neurosci. (2013)
3 ) Grasselli G et al., PLoS ONE (2011)
4 ) Spencer SA et al., J. Biol. Chem. (1992)
5 ) Rosenthal A et al., EMBO J. (1987)

Protocols

Standard protocol

ICC

IHC

Other products for this target

GAP43 antibody - 344 002

References for GAP43 - 344 002