c-Myc antibody - 343 011C3

c-Myc is a protein involved in the control of cell proliferation

Mouse monoclonal purified IgG

Cat. No.: 343 011C3

Amount: 100 µg

Price: $465.00

Cat. No. 343 011C3	100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster 550. Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established fluorescence detection systems. Oyster 550: λ_ex 551 nm / λ_em 570 nm Oyster 650: λ_ex 651 nm / λ_em 671 nm Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates. Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex^®(Merck Chemicals) is not compatible with Oyster dyes!
Applications	WB: N/A IP: N/A ICC: 1 : 500 gallery IHC: yes IHC-P: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Oyster 550
Clone	9E10
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 408 to 432 from human c-Myc (UniProt Id: P01106)
Epitop	AA 410 to 419 from human c-Myc (UniProt Id: P01106)
Reactivity	Reacts with: human (P01106). Other species not tested yet.
Data sheet	343_011c3.pdf

Cat. No.: 343 011C3

Amount: 100 µg

Price: $465.00

ICC

Background

The c-Myc oncogene (p62 c-Myc) is a nuclear protein that is involved in the control of normal cellular proliferation and differentiation. Deregulated expression of c-Myc induces apoptosis in different cell types.
The C-terminal c-Myc amino acids are widely used in combination with eukaryotic expression vectors encoding proteins with c-Myc epitope tag.

1 ) Kübler K et al., Oncotarget (2015)
2 ) Evan GI et al., Mol. Cell. Biol. (1985)
3 ) Dalla-Favera R et al., Proc. Natl. Acad. Sci. U.S.A. (1982)
4 ) Vennstrom B et al., J. Virol. (1982)

Protocols

ICC

IHC

IHC-P

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