c-Myc antibody - 343 011C2

c-Myc is a protein involved in the control of cell proliferation

Mouse monoclonal purified IgG

Cat. No.: 343 011C2

Amount: 100 µg

Price: $465.00

Cat. No. 343 011C2	100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 500 gallery IHC: yes IHC-P: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Cyanine 2
Clone	9E10
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 408 to 432 from human c-Myc (UniProt Id: P01106)
Epitop	AA 410 to 419 from human c-Myc (UniProt Id: P01106)
Reactivity	Reacts with: human (P01106). Other species not tested yet.
Data sheet	343_011c2.pdf

Cat. No.: 343 011C2

Amount: 100 µg

Price: $465.00

ICC

Background

The c-Myc oncogene (p62 c-Myc) is a nuclear protein that is involved in the control of normal cellular proliferation and differentiation. Deregulated expression of c-Myc induces apoptosis in different cell types.
The C-terminal c-Myc amino acids are widely used in combination with eukaryotic expression vectors encoding proteins with c-Myc epitope tag.

1 ) Kübler K et al., Oncotarget (2015)
2 ) Evan GI et al., Mol. Cell. Biol. (1985)
3 ) Dalla-Favera R et al., Proc. Natl. Acad. Sci. U.S.A. (1982)
4 ) Vennstrom B et al., J. Virol. (1982)

Protocols

ICC

IHC

IHC-P

Other products for this target