c-Myc antibody - 343 011BT

c-Myc is a protein involved in the control of cell proliferation

Mouse monoclonal purified IgG

Cat. No.: 343 011BT

Amount: 200 µg

Price: $465.00

Cat. No. 343 011BT	200 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 200 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: yes IHC-P: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	9E10
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 408 to 432 from human c-Myc (UniProt Id: P01106)
Epitop	AA 410 to 419 from human c-Myc (UniProt Id: P01106)
Reactivity	Reacts with: human (P01106). Other species not tested yet.
Data sheet	343_011bt.pdf

Cat. No.: 343 011BT

Amount: 200 µg

Price: $465.00

ICC

Background

The c-Myc oncogene (p62 c-Myc) is a nuclear protein that is involved in the control of normal cellular proliferation and differentiation. Deregulated expression of c-Myc induces apoptosis in different cell types.
The C-terminal c-Myc amino acids are widely used in combination with eukaryotic expression vectors encoding proteins with c-Myc epitope tag.

1 ) Kübler K et al., Oncotarget (2015)
2 ) Evan GI et al., Mol. Cell. Biol. (1985)
3 ) Dalla-Favera R et al., Proc. Natl. Acad. Sci. U.S.A. (1982)
4 ) Vennstrom B et al., J. Virol. (1982)

Protocols

Standard protocol

ICC

IHC

IHC-P

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