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Home > Products > 342 104

DAP1 antibody - 342 104

DAP1 is a post-synaptic scaffolding protein
Guinea pig polyclonal antiserum
Cat. No.: 342 104
Amount: 100 µl
Price: $365.00
Cat. No. 342 104 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
ExM: 1 : 50 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen Recombinant protein corresponding to AA 721 to 778 from mouse DAP1 (UniProt Id: Q9D415)
Reactivity Reacts with: rat (P97836), mouse (Q9D415).
Other species not tested yet.
Specificity Specific for DAP 1; detects all six isoforms known in rat and isoform 1, 2, 3 and 4 in mouse.
Remarks

ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
Data sheet 342_104.pdf
Cat. No.: 342 104
Amount: 100 µl
Price: $365.00
WB
ICC
ExM
Background

Disks large associated proteins (DLGAPs or DAPs) also known as SAP90/PSD-95-associated proteins (SAPAPs) are members of a protein family whose members interact with a membrane-associated guanylate kinase localized at the postsynaptic density (PSD) in neuronal cells. DAPs are adaptor proteins that also interact with other synaptic scaffolding proteins like shanks.