Synaptic Systems

Cat. No.: 328 005

Amount: 50 µg

Price: $460.00

Cat. No. 328 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 1000 up to 1 : 2000 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 128 to 145 from rat Tbr1 (UniProt Id: D4A6N8)
Reactivity	Reacts with: rat (D4A6N8), mouse (Q64336). Other species not tested yet.
Data sheet	328_005.pdf

References for Tbr1 - 328 005

Generation of excitatory and inhibitory neurons from common progenitors via Notch signaling in the cerebellum.
Zhang T, Liu T, Mora N, Guegan J, Bertrand M, Contreras X, Hansen AH, Streicher C, Anderle M, Danda N, Tiberi L, et al.
Cell reports (2021) 3510: 109208. 328 005 IHC; tested species: mouse

53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination.
Callen E, Zong D, Wu W, Wong N, Stanlie A, Ishikawa M, Pavani R, Dumitrache LC, Byrum AK, Mendez-Dorantes C, Martinez P, et al.
Molecular cell (2020) 771: 26-38.e7. 328 005 IHC; tested species: mouse

DNA-PKcs, ATM, and ATR Interplay Maintains Genome Integrity during Neurogenesis.
Enriquez-Rios V, Dumitrache LC, Downing SM, Li Y, Brown EJ, Russell HR, McKinnon PJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 374: 893-905. 328 005 IHC; tested species: mouse

Cat. No.: 328 005

Amount: 50 µg

Price: $460.00

ICC

IHC

IHC-P

Background

Tbr 1 is a transcription factor of the T-box family that is highly expressed in glutamatergic early-born cortical projection neurons. It plays a role in the differentiation of intermediate progenitor cells (IPCs) into postmitotic projection neuron.

1 ) Imamura F et al., Mol. Cell. Neurosci. (2013)
2 ) Méndez-Gómez HR et al., Mol. Cell. Neurosci. (2011)
3 ) Bedogni F et al., Proc. Natl. Acad. Sci. U.S.A. (2010)
4 ) Englund C et al., J. Neurosci. (2005)
5 ) Hevner RF et al., Neuron (2001)

Protocols

ICC

IHC

IHC-P

Other products for this target

Tbr1 antibody - 328 005

References for Tbr1 - 328 005