Tbr1 antibody - 328 003

Tbr1 is a transcription factor involved in neuronal differentiation

Rabbit polyclonal purified antibody

Cat. No.: 328 003

Amount: 50 µg

Price: $375.00

Cat. No. 328 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 128 to 145 from rat Tbr1 (UniProt Id: D4A6N8)
Reactivity	Reacts with: rat (D4A6N8), mouse (Q64336). Other species not tested yet.
Data sheet	328_003.pdf

Cat. No.: 328 003

Amount: 50 µg

Price: $375.00

ICC

IHC

IHC-P

Background

Tbr 1 is a transcription factor of the T-box family that is highly expressed in glutamatergic early-born cortical projection neurons. It plays a role in the differentiation of intermediate progenitor cells (IPCs) into postmitotic projection neuron.

1 ) Imamura F et al., Mol. Cell. Neurosci. (2013)
2 ) Méndez-Gómez HR et al., Mol. Cell. Neurosci. (2011)
3 ) Bedogni F et al., Proc. Natl. Acad. Sci. U.S.A. (2010)
4 ) Englund C et al., J. Neurosci. (2005)
5 ) Hevner RF et al., Neuron (2001)

Protocols

ICC

IHC

IHC-P

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