Synaptic Systems

Cat. No.: 327 004

Amount: 100 µl

Price: $365.00

Cat. No. 327 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 718 to 733 from mouse SATB2 (UniProt Id: Q8VI24)
Reactivity	Reacts with: rat (D3ZJ19), mouse (Q8VI24). Other species not tested yet.
Data sheet	327_004.pdf

References for SATB2 - 327 004

iPSC-derived models of PACS1 syndrome reveal transcriptional and functional deficits in neuron activity.
Rylaarsdam L, Rakotomamonjy J, Pope E, Guemez-Gamboa A
Nature communications (2024) 151: 827. 327 004 IHC; tested species: human

KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury.
Lai JD, Berlind JE, Fricklas G, Lie C, Urenda JP, Lam K, Sta Maria N, Jacobs R, Yu V, Zhao Z, Ichida JK, et al.
Cell stem cell (2024) 314: 519-536.e8. 327 004 IHC; tested species: human

Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons.
Liau ES, Jin S, Chen YC, Liu WS, Calon M, Nedelec S, Nie Q, Chen JA
Nature communications (2023) 141: 46. 327 004 IHC; tested species: mouse

MicroRNAs mediate precise control of spinal interneuron populations to exert delicate sensory-to-motor outputs.
Chang SH, Su YC, Chang M, Chen JA
eLife (2021) 10: . 327 004 IHC; tested species: mouse

A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons.
Okaty BW, Sturrock N, Escobedo Lozoya Y, Chang Y, Senft RA, Lyon KA, Alekseyenko OV, Dymecki SM
eLife (2020) 9: . 327 004 IHC; tested species: mouse

Inducing Different Neuronal Subtypes from Astrocytes in the Injured Mouse Cerebral Cortex.
Mattugini N, Bocchi R, Scheuss V, Russo GL, Torper O, Lao CL, Götz M
Neuron (2019) : . 327 004 IHC; tested species: mouse

Cat. No.: 327 004

Amount: 100 µl

Price: $365.00

ICC

IHC

IHC-P

Background

The Special AT-rich sequence-binding protein 2 or SATB 2 is a transcription factor required for the initiation of the genetic program for the upper-layer neurons (UL1).
Together with Ctip 2, Coup-TFI, and Fezf 2 it is involved in the fine tuned sequential formation and specification of the different excitatory neuron populations forming the definitive six-layered cortical structure.

1 ) Srivatsa S et al., Nat Commun (2014)
2 ) Díaz-Alonso J et al., J. Neurosci. (2012)
3 ) Apostolova G et al., J. Neurosci. (2010)
4 ) Gyorgy AB et al., Eur. J. Neurosci. (2008)

Protocols

ICC

IHC

IHC-P

Other products for this target

SATB2 antibody - 327 004

References for SATB2 - 327 004