Doublecortin antibody - 326 014

Doublecortin or DCX is a protein associated with neuronal differentiation

Guinea pig polyclonal antiserum

Cat. No.: 326 014

Amount: 100 µl

Price: $365.00

Cat. No. 326 014	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 2000 up to 1 : 10000 (AP-staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 1000 up to 1 : 2000 gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse Doublecortin (UniProt Id: Q6PGI2)
Reactivity	Reacts with: mouse (O88809). Other species not tested yet.
Data sheet	326_014.pdf

Cat. No.: 326 014

Amount: 100 µl

Price: $365.00

IHC

IHC-P

Background

Doublecortin or DCX seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Mutations in the gene encoding this protein have been linked to the double cortex syndrome.
Doublecortin is highly expressed in neuronal cells of fetal brain but absent from other tissues. In the adult dentate gyrus it is only expressed in cells contributing to adult neurogenesis.

1 ) Tang SK et al., Neuroscience (2014)
2 ) Plümpe T et al., BMC Neurosci (2006)
3 ) Moores CA et al., EMBO J. (2006)
4 ) Francis F et al., Neuron (1999)
5 ) Gleeson JG et al., Cell (1998)

Protocols

IHC

IHC-P

Other products for this target