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Doublecortin antibody - 326 003

Doublecortin or DCX is a protein associated with neuronal differentiation
Rabbit polyclonal purified antibody
Cat. No.: 326 003
Amount: 50 µg
Price: $380.00
Cat. No. 326 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 271 to 366 from mouse Doublecortin (UniProt Id: Q6PGI2)
Reactivity Reacts with: rat (Q9ESI7), mouse (Q6PGI2), human (O43602).
Other species not tested yet.
Data sheet 326_003.pdf

References for Doublecortin - 326 003

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia.
Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Riparbelli MG, Callaini G, Karch CM, Goureau O, Papantonis A, et al.
Nature protocols (2023) 186: 1893-1929. 326 003 ICC; tested species: human
Spatiotemporal Mapping and Molecular Basis of Whole-brain Circuit Maturation.
Xue J, Brawner AT, Thompson JR, Yelhekar TD, Newmaster KT, Qiu Q, Cooper YA, Yu CR, Ahmed-Braima YH, Kim Y, Lin Y, et al.
bioRxiv : the preprint server for biology (2024) : . 326 003 IHC; tested species: mouse
Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice.
Gallardo-Caballero M, Rodríguez-Moreno CB, Álvarez-Méndez L, Terreros-Roncal J, Flor-García M, Moreno-Jiménez EP, Rábano A, Llorens-Martín M
Communications biology (2023) 61: 978. 326 003 IHC; tested species: mouse
The Preventive Effect of Exercise and Oral Branched-Chain Amino Acid Supplementation on Obesity-Induced Brain Changes in Ldlr-/-.Leiden Mice.
Lohkamp KJ, van den Hoek AM, Solé-Guardia G, Lisovets M, Alves Hoffmann T, Velanaki K, Geenen B, Verweij V, Morrison MC, Kleemann R, Wiesmann M, et al.
Nutrients (2023) 157: . 326 003 IHC; tested species: mouse
The NOP antagonist BTRX-246040 increases stress resilience in mice without affecting adult neurogenesis in the hippocampus.
D'Oliveira da Silva F, Azevedo Neto J, Sturaro C, Guarino A, Robert C, Gavioli EC, Calo G, Mouledous L, Ruzza C
Neuropharmacology (2022) 212: 109077. 326 003 IHC; tested species: mouse
The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV.
Vollersen N, Zhao W, Rolvien T, Lange F, Schmidt FN, Sonntag S, Shmerling D, von Kroge S, Stockhausen KE, Sharaf A, Schweizer M, et al.
Bone research (2021) 91: 48. 326 003 IHC; tested species: mouse
Levetiracetam and N-Cadherin Antibody Alleviate Brain Pathology Without Reducing Early Epilepsy Development After Focal Non-convulsive Status Epilepticus in Rats.
Avdic U, Ahl M, Andersson M, Ekdahl CT
Frontiers in neurology (2021) 12: 630154. 326 003 IHC; tested species: rat
Human brain organoids assemble functionally integrated bilateral optic vesicles.
Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Schinzel F, Karch CM, Bao G, Gottardo M, Suren AA, et al.
Cell stem cell (2021) 2810: 1740-1757.e8. 326 003 IHC; tested species: human
A Citrus based sensory functional food ingredient induces antidepressant-like effects: possible involvement of an interplay between the olfactory and the serotonergic systems.
Coutens B, Rekik K, Harster A, Etienne P, Noirot V, Frances B, Moulédous L, Guiard BP
Neuroscience (2020) : . 326 003 IHC; tested species: mouse
In Vivo Reprogramming Ameliorates Aging Features in Dentate Gyrus Cells and Improves Memory in Mice.
Rodríguez-Matellán A, Alcazar N, Hernández F, Serrano M, Ávila J
Stem cell reports (2020) : . 326 003 IHC; tested species: mouse
Social behavior in mice following chronic optogenetic stimulation of hippocampal engrams.
Doucette E, Merfeld E, Leblanc H, Monasterio A, Cincotta C, Grella SL, Logan J, Ramirez S
Neurobiology of learning and memory (2020) 176: 107321. 326 003 IHC; tested species: mouse
Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice.
Mensching L, Djogo N, Keller C, Rading S, Karsak M
International journal of molecular sciences (2019) 2015: . 326 003 IHC; tested species: mouse
Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids.
Gabriel E, Ramani A, Karow U, Gottardo M, Natarajan K, Gooi LM, Goranci-Buzhala G, Krut O, Peters F, Nikolic M, Kuivanen S, et al.
Cell stem cell (2017) 203: 397-406.e5. 326 003 IHC; tested species: human
Cat. No.: 326 003
Amount: 50 µg
Price: $380.00
Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia.
Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Riparbelli MG, Callaini G, Karch CM, Goureau O, Papantonis A, et al.
Nature protocols (2023) 186: 1893-1929. 326 003 ICC; tested species: human
Spatiotemporal Mapping and Molecular Basis of Whole-brain Circuit Maturation.
Xue J, Brawner AT, Thompson JR, Yelhekar TD, Newmaster KT, Qiu Q, Cooper YA, Yu CR, Ahmed-Braima YH, Kim Y, Lin Y, et al.
bioRxiv : the preprint server for biology (2024) : . 326 003 IHC; tested species: mouse
Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice.
Gallardo-Caballero M, Rodríguez-Moreno CB, Álvarez-Méndez L, Terreros-Roncal J, Flor-García M, Moreno-Jiménez EP, Rábano A, Llorens-Martín M
Communications biology (2023) 61: 978. 326 003 IHC; tested species: mouse
The Preventive Effect of Exercise and Oral Branched-Chain Amino Acid Supplementation on Obesity-Induced Brain Changes in Ldlr-/-.Leiden Mice.
Lohkamp KJ, van den Hoek AM, Solé-Guardia G, Lisovets M, Alves Hoffmann T, Velanaki K, Geenen B, Verweij V, Morrison MC, Kleemann R, Wiesmann M, et al.
Nutrients (2023) 157: . 326 003 IHC; tested species: mouse
The NOP antagonist BTRX-246040 increases stress resilience in mice without affecting adult neurogenesis in the hippocampus.
D'Oliveira da Silva F, Azevedo Neto J, Sturaro C, Guarino A, Robert C, Gavioli EC, Calo G, Mouledous L, Ruzza C
Neuropharmacology (2022) 212: 109077. 326 003 IHC; tested species: mouse
The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV.
Vollersen N, Zhao W, Rolvien T, Lange F, Schmidt FN, Sonntag S, Shmerling D, von Kroge S, Stockhausen KE, Sharaf A, Schweizer M, et al.
Bone research (2021) 91: 48. 326 003 IHC; tested species: mouse
Levetiracetam and N-Cadherin Antibody Alleviate Brain Pathology Without Reducing Early Epilepsy Development After Focal Non-convulsive Status Epilepticus in Rats.
Avdic U, Ahl M, Andersson M, Ekdahl CT
Frontiers in neurology (2021) 12: 630154. 326 003 IHC; tested species: rat
Human brain organoids assemble functionally integrated bilateral optic vesicles.
Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Schinzel F, Karch CM, Bao G, Gottardo M, Suren AA, et al.
Cell stem cell (2021) 2810: 1740-1757.e8. 326 003 IHC; tested species: human
A Citrus based sensory functional food ingredient induces antidepressant-like effects: possible involvement of an interplay between the olfactory and the serotonergic systems.
Coutens B, Rekik K, Harster A, Etienne P, Noirot V, Frances B, Moulédous L, Guiard BP
Neuroscience (2020) : . 326 003 IHC; tested species: mouse
In Vivo Reprogramming Ameliorates Aging Features in Dentate Gyrus Cells and Improves Memory in Mice.
Rodríguez-Matellán A, Alcazar N, Hernández F, Serrano M, Ávila J
Stem cell reports (2020) : . 326 003 IHC; tested species: mouse
Social behavior in mice following chronic optogenetic stimulation of hippocampal engrams.
Doucette E, Merfeld E, Leblanc H, Monasterio A, Cincotta C, Grella SL, Logan J, Ramirez S
Neurobiology of learning and memory (2020) 176: 107321. 326 003 IHC; tested species: mouse
Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice.
Mensching L, Djogo N, Keller C, Rading S, Karsak M
International journal of molecular sciences (2019) 2015: . 326 003 IHC; tested species: mouse
Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids.
Gabriel E, Ramani A, Karow U, Gottardo M, Natarajan K, Gooi LM, Goranci-Buzhala G, Krut O, Peters F, Nikolic M, Kuivanen S, et al.
Cell stem cell (2017) 203: 397-406.e5. 326 003 IHC; tested species: human
WB
ICC
ICC
IHC
IHC
IHC-P
Background
Doublecortin or DCX seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Mutations in the gene encoding this protein have been linked to the double cortex syndrome.
Doublecortin is highly expressed in neuronal cells of fetal brain but absent from other tissues. In the adult dentate gyrus it is only expressed in cells contributing to adult neurogenesis.
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