Synaptic Systems - Doublecortin

Cat. No.: 326 003

Amount: 50 µg

Price: $385.00

Cat. No. 326 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P (FFPE): 1 : 200 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 271 to 366 from mouse Doublecortin (UniProt Id: Q6PGI2)
Reactivity	Reacts with: rat (Q9ESI7), mouse (Q6PGI2), human (O43602). Other species not tested yet.
Data sheet	Data Sheet 326_003 Safety Data Sheet (SDS)

References for Doublecortin - 326 003

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia.
Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Riparbelli MG, Callaini G, Karch CM, Goureau O, Papantonis A, et al.
Nature protocols (2023) 186: 1893-1929. 326 003 ICC; tested species: human

Chronic exposure to a synthetic cannabinoid improves cognition and increases locomotor activity in Tg4-42 Alzheimer's disease mice.
Ott FW, Sichler ME, Bouter C, Enayati M, Wiltfang J, Bayer TA, Beindorff N, Löw MJ, Bouter Y
Journal of Alzheimer's disease reports (2025) 9: 25424823241306770. 326 003 IHC; tested species: mouse

Toxoplasma gondii infection and chronic IL-1 elevation drive hippocampal DNA double-strand break signaling, leading to cognitive deficits.
Belloy M, Schmitt BAM, Marty FH, Paut C, Bassot E, Aïda A, Alis M, Zahm M, Chaubet A, Garnier H, Flores-Aguilar T, et al.
Nature neuroscience (2025) : . 326 003 IHC; tested species: mouse

Spatiotemporal Mapping and Molecular Basis of Whole-brain Circuit Maturation.
Xue J, Brawner AT, Thompson JR, Yelhekar TD, Newmaster KT, Qiu Q, Cooper YA, Yu CR, Ahmed-Braima YH, Kim Y, Lin Y, et al.
bioRxiv : the preprint server for biology (2024) : . 326 003 IHC; tested species: mouse

Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice.
Gallardo-Caballero M, Rodríguez-Moreno CB, Álvarez-Méndez L, Terreros-Roncal J, Flor-García M, Moreno-Jiménez EP, Rábano A, Llorens-Martín M
Communications biology (2023) 61: 978. 326 003 IHC; tested species: mouse

The Preventive Effect of Exercise and Oral Branched-Chain Amino Acid Supplementation on Obesity-Induced Brain Changes in Ldlr-/-.Leiden Mice.
Lohkamp KJ, van den Hoek AM, Solé-Guardia G, Lisovets M, Alves Hoffmann T, Velanaki K, Geenen B, Verweij V, Morrison MC, Kleemann R, Wiesmann M, et al.
Nutrients (2023) 157: . 326 003 IHC; tested species: mouse

The NOP antagonist BTRX-246040 increases stress resilience in mice without affecting adult neurogenesis in the hippocampus.
D'Oliveira da Silva F, Azevedo Neto J, Sturaro C, Guarino A, Robert C, Gavioli EC, Calo G, Mouledous L, Ruzza C
Neuropharmacology (2022) 212: 109077. 326 003 IHC; tested species: mouse

The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV.
Vollersen N, Zhao W, Rolvien T, Lange F, Schmidt FN, Sonntag S, Shmerling D, von Kroge S, Stockhausen KE, Sharaf A, Schweizer M, et al.
Bone research (2021) 91: 48. 326 003 IHC; tested species: mouse

Levetiracetam and N-Cadherin Antibody Alleviate Brain Pathology Without Reducing Early Epilepsy Development After Focal Non-convulsive Status Epilepticus in Rats.
Avdic U, Ahl M, Andersson M, Ekdahl CT
Frontiers in neurology (2021) 12: 630154. 326 003 IHC; tested species: rat

Human brain organoids assemble functionally integrated bilateral optic vesicles.
Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Schinzel F, Karch CM, Bao G, Gottardo M, Suren AA, et al.
Cell stem cell (2021) 2810: 1740-1757.e8. 326 003 IHC; tested species: human

A Citrus based sensory functional food ingredient induces antidepressant-like effects: possible involvement of an interplay between the olfactory and the serotonergic systems.
Coutens B, Rekik K, Harster A, Etienne P, Noirot V, Frances B, Moulédous L, Guiard BP
Neuroscience (2020) : . 326 003 IHC; tested species: mouse

In Vivo Reprogramming Ameliorates Aging Features in Dentate Gyrus Cells and Improves Memory in Mice.
Rodríguez-Matellán A, Alcazar N, Hernández F, Serrano M, Ávila J
Stem cell reports (2020) : . 326 003 IHC; tested species: mouse

Social behavior in mice following chronic optogenetic stimulation of hippocampal engrams.
Doucette E, Merfeld E, Leblanc H, Monasterio A, Cincotta C, Grella SL, Logan J, Ramirez S
Neurobiology of learning and memory (2020) 176: 107321. 326 003 IHC; tested species: mouse

Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice.
Mensching L, Djogo N, Keller C, Rading S, Karsak M
International journal of molecular sciences (2019) 2015: . 326 003 IHC; tested species: mouse

Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids.
Gabriel E, Ramani A, Karow U, Gottardo M, Natarajan K, Gooi LM, Goranci-Buzhala G, Krut O, Peters F, Nikolic M, Kuivanen S, et al.
Cell stem cell (2017) 203: 397-406.e5. 326 003 IHC; tested species: human

Cat. No.: 326 003

Amount: 50 µg

Price: $385.00

Background

Doublecortin (DCX) is a microtubule-associated protein that plays an important role in neuronal development, migration, and differentiation. It is predominantly expressed in immature neurons and neuronal precursor cells, where it contributes to the organization and stability of microtubules and thereby supports proper neuronal migration during development [1-3]. Moreover, it has been observed in the adult rostral migratory stream (RMS), which contains the neuronal precursors traveling to the olfactory bulb [4]. In addition to its developmental function, DCX is expressed transiently in immature neurons generated during adult neurogenesis. In experimental models, DCX-positive cells are found in neurogenic regions such as the dentate gyrus and subventricular zone, and DCX immunoreactivity decreases as newly generated neurons mature [5,6].
Mutations in the DCX gene cause severe abnormalities of cortical neuronal migration and have been linked to the double cortex syndrome [7].
DCX is widely used as a marker of immature neurons and adult neurogenesis. In the adult hippocampus, DCX-positive cells represent developing neurons at different stages of maturation.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Francis F et al., Neuron (1999)
2 ) Moores CA et al., EMBO J. (2006)
3 ) Dema A et al., Curr Biol (2024)
4 ) Tang SK et al., Neuroscience (2014)
5 ) Couillard-Despres S et al., Eur J Neurosci (2005)
6 ) Plümpe T et al., BMC Neurosci (2006)
7 ) Gleeson JG et al., Cell (1998)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Standard Condition

Other products for this target

Doublecortin antibody - 326 003

References for Doublecortin - 326 003