Synaptic Systems - Doublecortin

Cat. No.: 326 003

Amount: 50 µg

Price: $385.00

Cat. No. 326 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 500 gallery
Immunogen	Recombinant protein corresponding to AA 271 to 366 from mouse Doublecortin (UniProt Id: Q6PGI2)
Reactivity	Reacts with: rat (Q9ESI7), mouse (Q6PGI2), human (O43602). Other species not tested yet.
Data sheet	326_003.pdf

References for Doublecortin - 326 003

ICC (1)
IHC (13)

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia.
Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Riparbelli MG, Callaini G, Karch CM, Goureau O, Papantonis A, et al.
Nature protocols (2023) 186: 1893-1929. 326 003 ICC; tested species: human

Chronic exposure to a synthetic cannabinoid improves cognition and increases locomotor activity in Tg4-42 Alzheimer's disease mice.
Ott FW, Sichler ME, Bouter C, Enayati M, Wiltfang J, Bayer TA, Beindorff N, Löw MJ, Bouter Y
Journal of Alzheimer's disease reports (2025) 9: 25424823241306770. 326 003 IHC; tested species: mouse

Spatiotemporal Mapping and Molecular Basis of Whole-brain Circuit Maturation.
Xue J, Brawner AT, Thompson JR, Yelhekar TD, Newmaster KT, Qiu Q, Cooper YA, Yu CR, Ahmed-Braima YH, Kim Y, Lin Y, et al.
bioRxiv : the preprint server for biology (2024) : . 326 003 IHC; tested species: mouse

Prolonged fixation and post-mortem delay impede the study of adult neurogenesis in mice.
Gallardo-Caballero M, Rodríguez-Moreno CB, Álvarez-Méndez L, Terreros-Roncal J, Flor-García M, Moreno-Jiménez EP, Rábano A, Llorens-Martín M
Communications biology (2023) 61: 978. 326 003 IHC; tested species: mouse

The Preventive Effect of Exercise and Oral Branched-Chain Amino Acid Supplementation on Obesity-Induced Brain Changes in Ldlr-/-.Leiden Mice.
Lohkamp KJ, van den Hoek AM, Solé-Guardia G, Lisovets M, Alves Hoffmann T, Velanaki K, Geenen B, Verweij V, Morrison MC, Kleemann R, Wiesmann M, et al.
Nutrients (2023) 157: . 326 003 IHC; tested species: mouse

The NOP antagonist BTRX-246040 increases stress resilience in mice without affecting adult neurogenesis in the hippocampus.
D'Oliveira da Silva F, Azevedo Neto J, Sturaro C, Guarino A, Robert C, Gavioli EC, Calo G, Mouledous L, Ruzza C
Neuropharmacology (2022) 212: 109077. 326 003 IHC; tested species: mouse

The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV.
Vollersen N, Zhao W, Rolvien T, Lange F, Schmidt FN, Sonntag S, Shmerling D, von Kroge S, Stockhausen KE, Sharaf A, Schweizer M, et al.
Bone research (2021) 91: 48. 326 003 IHC; tested species: mouse

Levetiracetam and N-Cadherin Antibody Alleviate Brain Pathology Without Reducing Early Epilepsy Development After Focal Non-convulsive Status Epilepticus in Rats.
Avdic U, Ahl M, Andersson M, Ekdahl CT
Frontiers in neurology (2021) 12: 630154. 326 003 IHC; tested species: rat

Human brain organoids assemble functionally integrated bilateral optic vesicles.
Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Schinzel F, Karch CM, Bao G, Gottardo M, Suren AA, et al.
Cell stem cell (2021) 2810: 1740-1757.e8. 326 003 IHC; tested species: human

A Citrus based sensory functional food ingredient induces antidepressant-like effects: possible involvement of an interplay between the olfactory and the serotonergic systems.
Coutens B, Rekik K, Harster A, Etienne P, Noirot V, Frances B, Moulédous L, Guiard BP
Neuroscience (2020) : . 326 003 IHC; tested species: mouse

In Vivo Reprogramming Ameliorates Aging Features in Dentate Gyrus Cells and Improves Memory in Mice.
Rodríguez-Matellán A, Alcazar N, Hernández F, Serrano M, Ávila J
Stem cell reports (2020) : . 326 003 IHC; tested species: mouse

Social behavior in mice following chronic optogenetic stimulation of hippocampal engrams.
Doucette E, Merfeld E, Leblanc H, Monasterio A, Cincotta C, Grella SL, Logan J, Ramirez S
Neurobiology of learning and memory (2020) 176: 107321. 326 003 IHC; tested species: mouse

Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice.
Mensching L, Djogo N, Keller C, Rading S, Karsak M
International journal of molecular sciences (2019) 2015: . 326 003 IHC; tested species: mouse

Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids.
Gabriel E, Ramani A, Karow U, Gottardo M, Natarajan K, Gooi LM, Goranci-Buzhala G, Krut O, Peters F, Nikolic M, Kuivanen S, et al.
Cell stem cell (2017) 203: 397-406.e5. 326 003 IHC; tested species: human

Cat. No.: 326 003

Amount: 50 µg

Price: $385.00

References
ICC (1)
IHC (13)

ICC

IHC

IHC-P

Background

Doublecortin or DCX seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Mutations in the gene encoding this protein have been linked to the double cortex syndrome.
Doublecortin is highly expressed in neuronal cells of fetal brain but absent from other tissues. In the adult dentate gyrus it is only expressed in cells contributing to adult neurogenesis.

1 ) Tang SK et al., Neuroscience (2014)
2 ) Plümpe T et al., BMC Neurosci (2006)
3 ) Moores CA et al., EMBO J. (2006)
4 ) Francis F et al., Neuron (1999)
5 ) Gleeson JG et al., Cell (1998)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

Other products for this target

Doublecortin antibody - 326 003

References for Doublecortin - 326 003