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Ctip2 antibody C-terminus - 325 005

Ctip2 is a transcription factor involved in neuronal differentiation
Guinea pig polyclonal purified antibody
Cat. No.: 325 005
Amount: 50 µg
Price: $465.00
Cat. No. 325 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 541 to 812 from rat Ctip2 (UniProt Id: H9N1L3)
Reactivity Reacts with: rat (H9N1L3), mouse (Q99PV8).
Other species not tested yet.
Data sheet 325_005.pdf

References for Ctip2 - 325 005

Retrosplenial inputs drive visual representations in the medial entorhinal cortex.
Dubanet O, Higley MJ
Cell reports (2024) 437: 114470. 325 005 IHC; tested species: mouse
CaMKIIα Expressing Neurons to Report Activity-Related Endogenous Hypoxia upon Motor-Cognitive Challenge.
Butt UJ, Hassouna I, Fernandez Garcia-Agudo L, Steixner-Kumar AA, Depp C, Barnkothe N, Zillmann MR, Ronnenberg A, Bonet V, Goebbels S, Nave KA, et al.
International journal of molecular sciences (2021) 226: . 325 005 IHC; tested species: mouse
Brain erythropoietin fine-tunes a counterbalance between neurodifferentiation and microglia in the adult hippocampus.
Fernandez Garcia-Agudo L, Steixner-Kumar AA, Curto Y, Barnkothe N, Hassouna I, Jähne S, Butt UJ, Grewe K, Weber MS, Green K, Rizzoli S, et al.
Cell reports (2021) 368: 109548. 325 005 IHC; tested species: mouse
Functional hypoxia drives neuroplasticity and neurogenesis via brain erythropoietin.
Wakhloo D, Scharkowski F, Curto Y, Javed Butt U, Bansal V, Steixner-Kumar AA, Wüstefeld L, Rajput A, Arinrad S, Zillmann MR, Seelbach A, et al.
Nature communications (2020) 111: 1313. 325 005 IHC; tested species: mouse
Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.
Hassouna I, Ott C, Wüstefeld L, Offen N, Neher RA, Mitkovski M, Winkler D, Sperling S, Fries L, Goebbels S, Vreja IC, et al.
Molecular psychiatry (2016) 2112: 1752-1767. 325 005 IHC
Cat. No.: 325 005
Amount: 50 µg
Price: $465.00
Retrosplenial inputs drive visual representations in the medial entorhinal cortex.
Dubanet O, Higley MJ
Cell reports (2024) 437: 114470. 325 005 IHC; tested species: mouse
CaMKIIα Expressing Neurons to Report Activity-Related Endogenous Hypoxia upon Motor-Cognitive Challenge.
Butt UJ, Hassouna I, Fernandez Garcia-Agudo L, Steixner-Kumar AA, Depp C, Barnkothe N, Zillmann MR, Ronnenberg A, Bonet V, Goebbels S, Nave KA, et al.
International journal of molecular sciences (2021) 226: . 325 005 IHC; tested species: mouse
Brain erythropoietin fine-tunes a counterbalance between neurodifferentiation and microglia in the adult hippocampus.
Fernandez Garcia-Agudo L, Steixner-Kumar AA, Curto Y, Barnkothe N, Hassouna I, Jähne S, Butt UJ, Grewe K, Weber MS, Green K, Rizzoli S, et al.
Cell reports (2021) 368: 109548. 325 005 IHC; tested species: mouse
Functional hypoxia drives neuroplasticity and neurogenesis via brain erythropoietin.
Wakhloo D, Scharkowski F, Curto Y, Javed Butt U, Bansal V, Steixner-Kumar AA, Wüstefeld L, Rajput A, Arinrad S, Zillmann MR, Seelbach A, et al.
Nature communications (2020) 111: 1313. 325 005 IHC; tested species: mouse
Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.
Hassouna I, Ott C, Wüstefeld L, Offen N, Neher RA, Mitkovski M, Winkler D, Sperling S, Fries L, Goebbels S, Vreja IC, et al.
Molecular psychiatry (2016) 2112: 1752-1767. 325 005 IHC
Background

The Coup-TFI interacting protein 2 (Ctip 2), also referred to as Bcl 11b, is a zinc finger transcription factor that is expressed in postmitotic neurons.
Together with Satb 2, Coup-TFI, and Fezf2 it is involved in the fine tuned sequential formation and specification of the different excitatory neuron populations forming the definitive six-layered cortical structure.