Synaptic Systems

Cat. No.: 324 002

Amount: 200 µl

Price: $360.00

Cat. No. 324 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 40 to 94 from human WRB (UniProt Id: O00258)
Reactivity	Reacts with: human (O00258), rat (Q6P6S5), mouse (Q8K0D7). Other species not tested yet.
Specificity	K.D. validated PubMed: 31182645
Data sheet	Datasheet 324_002

References for WRB - 324 002

The GET insertase exhibits conformational plasticity and induces membrane thinning.
McDowell MA, Heimes M, Enkavi G, Farkas Á, Saar D, Wild K, Schwappach B, Vattulainen I, Sinning I
Nature communications (2023) 141: 7355. 324 002 WB

Differential Modes of Orphan Subunit Recognition for the WRB/CAML Complex.
Inglis AJ, Page KR, Guna A, Voorhees RM
Cell reports (2020) 3011: 3691-3698.e5. 324 002 WB; tested species: human

A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 324 002 WB; KD verified; tested species: human

Cat. No.: 324 002

Amount: 200 µl

Price: $360.00

Differential Modes of Orphan Subunit Recognition for the WRB/CAML Complex.
Inglis AJ, Page KR, Guna A, Voorhees RM
Cell reports (2020) 3011: 3691-3698.e5. 324 002 WB; tested species: human

Background

The widely expressed tryptophan (W)-rich basic protein (WRB), also known as congenital heart disease protein 5 (CHD 5) has initially been described as a nuclear protein that plays a potential role in the pathogenesis of CHD in down syndrome patients.
Recently this protein has been identified as the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.

1 ) Vilardi F et al., PLoS ONE (2014)
2 ) Vilardi F et al., J. Cell. Sci. (2011)
3 ) Egeo A et al., Hum. Genet. (1998)

Protocols

Other products for this target

WRB antibody - 324 002 K.D.

References for WRB - 324 002