Synaptic Systems

Cat. No.: 314 006

Amount: 200 µl

Price: $360.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Spatiotemporal analysis of axonal autophagosome-lysosome dynamics reveals limited fusion events and slow maturation.
Cason SE, Mogre SS, Holzbaur ELF, Koslover EF
Molecular biology of the cell (2022) 3313: ar123. 314 006 ICC; tested species: rat

WB

ICC

IHC

IHC-P

Background

There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and tau (55-65 kD). Both protein classes are involved in the regulation of microtubule polymerization in cells. Tau is a neuronal protein that mainly localizes to axons. Hyperphosphorylated tau has been shown to be a major element of paired helical filaments in Alzheimer's disease.

1 ) Thies E et al., J. Neurosci. (2007)
2 ) Avila J et al., J. Biomed. Biotechnol. (2006)
3 ) Duka T et al., FASEB J. (2006)
4 ) Black MM et al., J. Neurosci. (1996)
5 ) Mandell JW et al., J. Neurosci. (1996)
6 ) Hirokawa N et al., J. Cell Biol. (1988)
7 ) Connolly JA et al., Proc. Natl. Acad. Sci. U.S.A. (1977)
8 ) Witman GB et al., Proc. Natl. Acad. Sci. U.S.A. (1976)

Protocols

WB

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Cat. No. 314 006	200 µl antibody, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to the N-terminal half of mouse Tau-D (UniProt Id: P10637-5)
Reactivity	Reacts with: rat, mouse (P10637-5). Other species not tested yet.
Specificity	This antibody binds phosphorylated and non-phosphorylated tau proteins. The sequence used for immunization is present in all splice variants except human TauA (UniProt Id: P10636-3).
Matching control protein/peptide	314-0P
Remarks	For human tissue cat.no. 314 012 and 314 111 are highly recommended.
Data sheet	314_006.pdf

Tau antibody - 314 006

References for Tau - 314 006