Synaptic Systems

Cat. No.: 314 002

Amount: 200 µl

Price: $355.00

Cat. No. 314 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 1000 gallery IHC: yes gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to the N-terminal half of mouse Tau-D (UniProt Id: P10637-5)
Reactivity	Reacts with: rat (P19332), mouse (P10637). Weaker signal: human (P10636). Other species not tested yet.
Specificity	This antibody binds phosphorylated and non-phosphorylated tau proteins. The sequence used for immunization is present in all splice variants except human TauA (UniProt Id: P10636-3).
Matching control protein/peptide	314-0P
Remarks	For human tissue cat.no. 314 012 and 314 111 are highly recommended.
Data sheet	314_002.pdf

References for Tau - 314 002

The frontotemporal dementia mutation R406W blocks tau's interaction with the membrane in an annexin A2-dependent manner.
Gauthier-Kemper A, Weissmann C, Golovyashkina N, Sebö-Lemke Z, Drewes G, Gerke V, Heinisch JJ, Brandt R
The Journal of cell biology (2011) 1924: 647-61. 314 002 WB, ICC

Vinculin-mediated axon growth requires interaction with actin but not talin in mouse neocortical neurons.
Mandal P, Belapurkar V, Nair D, Ramanan N
Cellular and molecular life sciences : CMLS (2021) 7815: 5807-5826. 314 002 ICC; tested species: mouse

The role of ubiquitin ligase E3A in polarized contact guidance and rescue strategies in UBE3A-deficient hippocampal neurons.
Tonazzini I, Van Woerden GM, Masciullo C, Mientjes EJ, Elgersma Y, Cecchini M
Molecular autism (2019) 10: 41. 314 002 ICC; tested species: mouse

Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones.
Vysokov NV, Silva JP, Lelianova VG, Suckling J, Cassidy J, Blackburn JK, Yankova N, Djamgoz MB, Kozlov SV, Tonevitsky AG, Ushkaryov YA, et al.
eLife (2018) 7: . 314 002 ICC; tested species: rat

HuD Is a Neural Translation Enhancer Acting on mTORC1-Responsive Genes and Counteracted by the Y3 Small Non-coding RNA.
Tebaldi T, Zuccotti P, Peroni D, Köhn M, Gasperini L, Potrich V, Bonazza V, Dudnakova T, Rossi A, Sanguinetti G, Conti L, et al.
Molecular cell (2018) 712: 256-270.e10. 314 002 ICC; tested species: mouse

Biocompatibility of a Magnetic Tunnel Junction Sensor Array for the Detection of Neuronal Signals in Culture.
Moretti D, DiFrancesco ML, Sharma PP, Dante S, Albisetti E, Monticelli M, Bertacco R, Petti D, Baldelli P, Benfenati F
Frontiers in neuroscience (2018) 12: 909. 314 002 ICC; tested species: rat

CRMP1 and CRMP2 have synergistic but distinct roles in dendritic development.
Makihara H, Nakai S, Ohkubo W, Yamashita N, Nakamura F, Kiyonari H, Shioi G, Jitsuki-Takahashi A, Nakamura H, Tanaka F, Akase T, et al.
Genes to cells : devoted to molecular & cellular mechanisms (2016) 219: 994-1005. 314 002 ICC; tested species: mouse

Mammalian target of rapamycin (mTOR) activation increases axonal growth capacity of injured peripheral nerves.
Abe N, Borson SH, Gambello MJ, Wang F, Cavalli V
The Journal of biological chemistry (2010) 28536: 28034-43. 314 002 ICC

Cat. No.: 314 002

Amount: 200 µl

Price: $355.00

ICC

IHC

Background

There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and tau (55-65 kD). Both protein classes are involved in the regulation of microtubule polymerization in cells. Tau is a neuronal protein that mainly localizes to axons. Hyperphosphorylated tau has been shown to be a major element of paired helical filaments in Alzheimer's disease.

1 ) Thies E et al., J. Neurosci. (2007)
2 ) Avila J et al., J. Biomed. Biotechnol. (2006)
3 ) Duka T et al., FASEB J. (2006)
4 ) Black MM et al., J. Neurosci. (1996)
5 ) Mandell JW et al., J. Neurosci. (1996)
6 ) Hirokawa N et al., J. Cell Biol. (1988)
7 ) Connolly JA et al., Proc. Natl. Acad. Sci. U.S.A. (1977)
8 ) Witman GB et al., Proc. Natl. Acad. Sci. U.S.A. (1976)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

Tau antibody - 314 002

References for Tau - 314 002