Cortactin antibody - 313 111

Cortactin is a cortical actin binding protein

Mouse monoclonal purified IgG

Cat. No.: 313 111

Amount: 100 µg

Price: $415.00

Cat. No. 313 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: yes (see remarks) gallery IHC: not tested yet IHC-P: not tested yet ELISA: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	289H10
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 513 from human Cortactin (UniProt Id: Q14247)
Epitop	AA 147 to 509 from human Cortactin (UniProt Id: Q14247)
Reactivity	Reacts with: human (Q14247), mouse (Q60598). Other species not tested yet.
Remarks	ICC: Works on PFA and methanol fixed cells, but methanol fixation produces less background.
Data sheet	313_111.pdf

References for Cortactin - 313 111

Cortactin promotes migration and platelet-derived growth factor-induced actin reorganization by signaling to Rho-GTPases.
Lai FP, Szczodrak M, Oelkers JM, Ladwein M, Acconcia F, Benesch S, Auinger S, Faix J, Small JV, Polo S, Stradal TE, et al.
Molecular biology of the cell (2009) 2014: 3209-23. 313 111 ICC

Cat. No.: 313 111

Amount: 100 µg

Price: $415.00

ICC

Background

Cortactin is a cortical actin binding protein and the major substrate for the tyrosine kinase v-Src. The interaction of its N-terminal acidic domain (NTA) with the actin nucleation factor Arp 2/3 links cytoskeletal organization with signal transduction. The carboxy terminus comprises a proline rich region and an SH3 domain that can interact with several scaffolding proteins like CortBP1 and Shank 3. In neurons cortactin is concentrated in dendritic spines and involved in spine targeting.

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2 ) Tehrani S et al., Proc. Natl. Acad. Sci. U.S.A. (2007)
3 ) Sousa S et al., Cell. Microbiol. (2007)
4 ) Cosen-Binker LI et al., Physiology (Bethesda) (2006)
5 ) Kim K et al., Biochemistry (2004)
6 ) Helwani FM et al., J. Cell Biol. (2004)
7 ) Redecker P et al., J. Histochem. Cytochem. (2001)
8 ) Olazabal IM et al., J. Cell Biol. (2001)
9 ) Kinnunen T et al., J. Biol. Chem. (1998)
10 ) Peng HB et al., J. Neurocytol. (1997)

Protocols

ICC

ELISA

Standard protocol

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