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Nestin - 312 011

A class VI intermediate filament
Monoclonal mouse purified IgG
Cat. No.: 312 011
Amount: 100 µg
Price: 415.00 €
Cat. No. 312 011 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: yes gallery  
IHC: yes
IHC-P/FFPE: not tested yet
ELISA: yes
Clone JP63
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 1618 from human Nestin (UniProt Id: P48681)
Epitop Epitop: AA 1 to 1618 from human Nestin (UniProt Id: P48681)
Reactivity Reacts with: human (P48681), monkey.
Other species not tested yet.
Specificity Specific for human nestin.
Remarks

Differences between clone JP 63 and clone JP39 in application or specificity not determined yet.

Data sheet 312_011.pdf

References for Nestin - 312 011

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 312 011 WB; tested species: rat
NDRG2 as a marker protein for brain astrocytes.
Flügge G, Araya-Callis C, Garea-Rodriguez E, Stadelmann-Nessler C, Fuchs E
Cell and tissue research (2014) 3571: 31-41. 312 011 IHC; tested species: marmoset
Cat. No.: 312 011
Quantity: 100 µg
Price: 415.00 €
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 312 011 WB; tested species: rat
NDRG2 as a marker protein for brain astrocytes.
Flügge G, Araya-Callis C, Garea-Rodriguez E, Stadelmann-Nessler C, Fuchs E
Cell and tissue research (2014) 3571: 31-41. 312 011 IHC; tested species: marmoset
Background
Nestin is expressed in early embryonic neuroepithelial stem cells during development of the CNS. The protein is downregulated and replaced by other tissue specific intermediate filaments during differentiation. Antibodies against this protein can be employed as predominant markers for stem and progenitor cells in the mammalian CNS.
Nestin is also a useful marker-protein for brain tumors of both glial and neuronal lineage.