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Home > Products > 311 011

MLC-2A antibody - 311 011

Myosin light chain 2 or MLC-2A is specific for the atrium of the mammalian heart
Mouse monoclonal purified IgG
Cat. No.: 311 011
Amount: 100 µg
Price: $430.00
Cat. No. 311 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: yes (see remarks) gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 56F5
Subtype IgG2b (κ light chain)
Immunogen Full-length recombinant human MLC-2A (UniProt Id: Q01449)
Reactivity Reacts with: human (Q01449), rat, mouse (Q9QVP4).
No signal: chicken.
Other species not tested yet.
Specificity Specific for MLC-2A, no cross-reactivity to MLC-2V.
Matching control protein/peptide 311-0P
Remarks

ICC: Methanol fixation gives stronger signals.
Data sheet 311_011.pdf

References for MLC-2A - 311 011

High-throughput longitudinal electrophysiology screening of mature chamber-specific hiPSC-CMs using optical mapping.
Allan A, Creech J, Hausner C, Krajcarski P, Gunawan B, Poulin N, Kozlowski P, Clark CW, Dow R, Saraithong P, Mair DB, et al.
iScience (2023) 267: 107142. 311 011 WB; tested species: human
JAK2 as a surface marker for enrichment of human pluripotent stem cells-derived ventricular cardiomyocytes.
Liew LC, Poh BM, An O, Ho BX, Lim CYY, Pang JKS, Beh LY, Yang HH, Soh BS
Stem cell research & therapy (2023) 141: 367. 311 011 WB, ICC; tested species: human
Myosin essential light chain 1sa decelerates actin and thin filament gliding on β-myosin molecules.
Osten J, Mohebbi M, Uta P, Matinmehr F, Wang T, Kraft T, Amrute-Nayak M, Scholz T
The Journal of general physiology (2022) 15410: . 311 011 WB; tested species: rabbit
LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells.
Song MH, Choi SC, Noh JM, Joo HJ, Park CY, Cha JJ, Ahn TH, Ko TH, Choi JI, Na JE, Rhyu IJ, et al.
Biomaterials (2021) 278: 121133. 311 011 WB, ICC, FACS; tested species: human
Phosphorylation and translocation of heat shock protein 27 and alphaB-crystallin in human myocardium after cardioplegia and cardiopulmonary bypass.
Clements RT, Sodha NR, Feng J, Mieno S, Boodhwani M, Ramlawi B, Bianchi C, Sellke FW
The Journal of thoracic and cardiovascular surgery (2007) 1346: 1461-70. 311 011 WB, IHC; tested species: human
Immune response caused by M1 macrophages elicits atrial fibrillation-like phenotypes in coculture model with isogenic hiPSC-derived cardiomyocytes.
Hutschalik T, Özgül O, Casini M, Szabó B, Peyronnet R, Bártulos Ó, Argenziano M, Schotten U, Matsa E
Stem cell research & therapy (2024) 151: 280. 311 011 ICC; tested species: human
CD151 expression marks atrial- and ventricular- differentiation from human induced pluripotent stem cells.
Nakanishi-Koakutsu M, Miki K, Naka Y, Sasaki M, Wakimizu T, Napier SC, Okubo C, Narita M, Nishikawa M, Hata R, Chonabayashi K, et al.
Communications biology (2024) 71: 231. 311 011 ICC; tested species: human
JAK2 as a surface marker for enrichment of human pluripotent stem cells-derived ventricular cardiomyocytes.
Liew LC, Poh BM, An O, Ho BX, Lim CYY, Pang JKS, Beh LY, Yang HH, Soh BS
Stem cell research & therapy (2023) 141: 367. 311 011 WB, ICC; tested species: human
Establishment of a protocol to administer immunosuppressive drugs for iPS cell-derived cardiomyocyte patch transplantation in a rat myocardial infarction model.
Ito E, Kawamura A, Kawamura T, Takeda M, Harada A, Mochizuki-Oda N, Sawa Y, Miyagawa S
Scientific reports (2023) 131: 10530. 311 011 ICC; tested species: human
Temporal Control of the WNT Signaling Pathway During Cardiac Differentiation Impacts Upon the Maturation State of Human Pluripotent Stem Cell Derived Cardiomyocytes.
Tsoi C, Deng R, Kwok M, Yan B, Lee C, Li HS, Ma CHY, Luo R, Leung KT, Chan GC, Chow LM, et al.
Frontiers in molecular biosciences (2022) 9: 714008. 311 011 ICC; tested species: human
Ultraefficient extracellular vesicle-guided direct reprogramming of fibroblasts into functional cardiomyocytes.
Kim H, Song BW, Park SJ, Choi SW, Moon H, Hwang KC, Kang SW, Moon SH, Yang Y, Kwon IC, Kim SH, et al.
Science advances (2022) 88: eabj6621. 311 011 ICC; tested species: mouse
An iPS-derived in vitro model of human atrial conduction.
Biendarra-Tiegs SM, Yechikov S, Shergill B, Brumback B, Takahashi K, Shirure VS, Gonzalez RE, Houshmand L, Zhong D, Weng KC, Silva J, et al.
Physiological reports (2022) 1018: e15407. 311 011 ICC; tested species: human
LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells.
Song MH, Choi SC, Noh JM, Joo HJ, Park CY, Cha JJ, Ahn TH, Ko TH, Choi JI, Na JE, Rhyu IJ, et al.
Biomaterials (2021) 278: 121133. 311 011 WB, ICC, FACS; tested species: human
Chamber-Specific Protein Expression during Direct Cardiac Reprogramming.
Zhang Z, Villalpando J, Zhang W, Nam YJ
Cells (2021) 106: . 311 011 ICC; tested species: mouse
Cell states beyond transcriptomics: Integrating structural organization and gene expression in hiPSC-derived cardiomyocytes.
Gerbin KA, Grancharova T, Donovan-Maiye RM, Hendershott MC, Anderson HG, Brown JM, Chen J, Dinh SQ, Gehring JL, Johnson GR, Lee H, et al.
Cell systems (2021) 126: 670-687.e10. 311 011 ICC; tested species: human
Spatial Stem Cell Fate Engineering via Facile Morphogen Localization.
Jin G, Floy ME, Simmons AD, Arthur MM, Palecek SP
Advanced healthcare materials (2021) 1021: e2100995. 311 011 ICC; tested species: human
Modeling Hypoxic Stress In Vitro Using Human Embryonic Stem Cells Derived Cardiomyocytes Matured by FGF4 and Ascorbic Acid Treatment.
Choi SC, Seo HR, Cui LH, Song MH, Noh JM, Kim KS, Choi JH, Kim JH, Park CY, Joo HJ, Hong SJ, et al.
Cells (2021) 1010: . 311 011 ICC; tested species: human
Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells.
Fukushima H, Yoshioka M, Kawatou M, López-Dávila V, Takeda M, Kanda Y, Sekino Y, Yoshida Y, Yamashita JK
PloS one (2020) 1511: e0241287. 311 011 ICC; tested species: human
3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells.
Branco MA, Dias TP, Cotovio JP, Rodrigues CAV, Fernandes TG, Cabral JMS, Diogo MM
Methods in molecular biology (Clifton, N.J.) (2020) : . 311 011 ICC; tested species: human
Continuous WNT Control Enables Advanced hPSC Cardiac Processing and Prognostic Surface Marker Identification in Chemically Defined Suspension Culture.
Halloin C, Schwanke K, Löbel W, Franke A, Szepes M, Biswanath S, Wunderlich S, Merkert S, Weber N, Osten F, de la Roche J, et al.
Stem cell reports (2019) 132: 366-379. 311 011 ICC, FACS; tested species: human
Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity.
Atmanli A, Hu D, Deiman FE, van de Vrugt AM, Black LD, Domian I
eLife (2019) 8: . 311 011 ICC; tested species: human
Generation of a Urine-Derived Ips Cell Line from a Patient with a Ventricular Septal Defect and Heart Failure and the Robust Differentiation of These Cells to Cardiomyocytes via Small Molecules.
Cao Y, Xu J, Wen J, Ma X, Liu F, Li Y, Chen W, Sun L, Wu Y, Li S, Li J, et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2018) 502: 538-551. 311 011 ICC; tested species: mouse
Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes.
Lin Y, Linask KL, Mallon B, Johnson K, Klein M, Beers J, Xie W, Du Y, Liu C, Lai Y, Zou J, et al.
Stem cells translational medicine (2017) 62: 527-538. 311 011 ICC; tested species: human
Use of Human Pluripotent Stem Cell Derived-Cardiomyocytes to Study Drug-Induced Cardiotoxicity.
Maillet A, Tan KP, Brunham LR
Current protocols in toxicology (2017) 73: 22.5.1-22.5.22. 311 011 ICC; tested species: human
Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos.
Lalit PA, Rodriguez AM, Downs KM, Kamp TJ
Nature protocols (2017) 125: 1029-1054. 311 011 ICC
Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells.
Pei F, Jiang J, Bai S, Cao H, Tian L, Zhao Y, Yang C, Dong H, Ma Y
Stem cell research (2017) 19: 94-103. 311 011 ICC
Generation of clinical-grade functional cardiomyocytes from human embryonic stem cells in chemically defined conditions.
Tan Y, Han P, Gu Q, Chen G, Wang L, Ma R, Wu J, Feng C, Zhang Y, Wang L, Hu B, et al.
Journal of tissue engineering and regenerative medicine (2016) : . 311 011 ICC; tested species: human
Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.
Dorn T, Goedel A, Lam JT, Haas J, Tian Q, Herrmann F, Bundschu K, Dobreva G, Schiemann M, Dirschinger R, Guo Y, et al.
Stem cells (Dayton, Ohio) (2015) 334: 1113-29. 311 011 ICC, IHC
Fibroblast Growth Factors and Vascular Endothelial Growth Factor Promote Cardiac Reprogramming under Defined Conditions.
Yamakawa H, Muraoka N, Miyamoto K, Sadahiro T, Isomi M, Haginiwa S, Kojima H, Umei T, Akiyama M, Kuishi Y, Kurokawa J, et al.
Stem cell reports (2015) 56: 1128-1142. 311 011 ICC
Cardiac atrial appendage stem cells engraft and differentiate into cardiomyocytes in vivo: A new tool for cardiac repair after MI.
Fanton Y, Robic B, Rummens JL, Daniëls A, Windmolders S, Willems L, Jamaer L, Dubois J, Bijnens E, Heuts N, Notelaers K, et al.
International journal of cardiology (2015) 201: 10-9. 311 011 ICC
Phasic modulation of Wnt signaling enhances cardiac differentiation in human pluripotent stem cells by recapitulating developmental ontogeny.
Mehta A, Ramachandra CJ, Sequiera GL, Sudibyo Y, Nandihalli M, Yong PJ, Koh CH, Shim W
Biochimica et biophysica acta (2014) 184311: 2394-402. 311 011 ICC
Chemically defined generation of human cardiomyocytes.
Burridge PW, Matsa E, Shukla P, Lin ZC, Churko JM, Ebert AD, Lan F, Diecke S, Huber B, Mordwinkin NM, Plews JR, et al.
Nature methods (2014) 118: 855-60. 311 011 ICC
Dantrolene rescues arrhythmogenic RYR2 defect in a patient-specific stem cell model of catecholaminergic polymorphic ventricular tachycardia.
Jung CB, Moretti A, Mederos y Schnitzler M, Iop L, Storch U, Bellin M, Dorn T, Ruppenthal S, Pfeiffer S, Goedel A, Dirschinger RJ, et al.
EMBO molecular medicine (2012) 43: 180-91. 311 011 ICC; tested species: human
Simultaneous voltage and calcium mapping of genetically purified human induced pluripotent stem cell-derived cardiac myocyte monolayers.
Lee P, Klos M, Bollensdorff C, Hou L, Ewart P, Kamp TJ, Zhang J, Bizy A, Guerrero-Serna G, Kohl P, Jalife J, et al.
Circulation research (2012) 11012: 1556-63. 311 011 ICC, FACS
Tbx5 overexpression favors a first heart field lineage in murine embryonic stem cells and in Xenopus laevis embryos.
Herrmann F, Bundschu K, Kühl SJ, Kühl M
Developmental dynamics : an official publication of the American Association of Anatomists (2011) 24012: 2634-45. 311 011 ICC
NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.
Elliott DA, Braam SR, Koutsis K, Ng ES, Jenny R, Lagerqvist EL, Biben C, Hatzistavrou T, Hirst CE, Yu QC, Skelton RJ, et al.
Nature methods (2011) 812: 1037-40. 311 011 ICC
Cardiomyocyte enrichment from human embryonic stem cell cultures by selection of ALCAM surface expression.
Rust W, Balakrishnan T, Zweigerdt R
Regenerative medicine (2009) 42: 225-37. 311 011 ICC
Functional cardiomyocytes derived from human induced pluripotent stem cells.
Zhang J, Wilson GF, Soerens AG, Koonce CH, Yu J, Palecek SP, Thomson JA, Kamp TJ
Circulation research (2009) 1044: e30-41. 311 011 ICC
Highly enriched cardiomyocytes from human embryonic stem cells.
Xu XQ, Zweigerdt R, Soo SY, Ngoh ZX, Tham SC, Wang ST, Graichen R, Davidson B, Colman A, Sun W
Cytotherapy (2008) 104: 376-89. 311 011 ICC, IHC
Cardiomyocytes from human and mouse embryonic stem cells.
Mummery C, van der Heyden MA, de Boer TP, Passier R, Ward D, van den Brink S, van Rooijen M, van de Stolpe A
Methods in molecular medicine (2007) 140: 249-72. 311 011 ICC; tested species: human,mouse
Scalable production of homogeneous cardiac organoids derived from human pluripotent stem cells.
Moriwaki T, Tani H, Haga K, Morita-Umei Y, Soma Y, Umei TC, Sekine O, Takatsuna K, Kishino Y, Kanazawa H, Fujita J, et al.
Cell reports methods (2023) 312: 100666. 311 011 IHC; tested species: human
Development of In Vitro Drug-Induced Cardiotoxicity Assay by Using Three-Dimensional Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells.
Takeda M, Miyagawa S, Fukushima S, Saito A, Ito E, Harada A, Matsuura R, Iseoka H, Sougawa N, Mochizuki-Oda N, Matsusaki M, et al.
Tissue engineering. Part C, Methods (2018) 241: 56-67. 311 011 IHC; tested species: stem cells
Meticulous optimization of cardiomyocyte yields in a 3-stage continuous integrated agitation bioprocess.
Ting S, Lam A, Tong G, Chen A, Wei H, Wu J, Lam YN, Reuveny S, Oh S
Stem cell research (2018) 31: 161-173. 311 011 IHC; tested species: human
Human Embryonic Stem Cell-Derived Cardiomyocytes Self-Arrange with Areas of Different Subtypes During Differentiation.
Vestergaard ML, Grubb S, Koefoed K, Anderson-Jenkins Z, Grunnet-Lauridsen K, Calloe K, Clausen C, Christensen ST, Møllgård K, Andersen CY
Stem cells and development (2017) 2621: 1566-1577. 311 011 IHC; tested species: human
Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.
Dorn T, Goedel A, Lam JT, Haas J, Tian Q, Herrmann F, Bundschu K, Dobreva G, Schiemann M, Dirschinger R, Guo Y, et al.
Stem cells (Dayton, Ohio) (2015) 334: 1113-29. 311 011 ICC, IHC
Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications.
Naaijkens BA, Niessen HW, Prins HJ, Krijnen PA, Kokhuis TJ, de Jong N, van Hinsbergh VW, Kamp O, Helder MN, Musters RJ, van Dijk A, et al.
Cell and tissue research (2012) 3481: 119-30. 311 011 IHC
Human cardiomyocyte progenitor cell transplantation preserves long-term function of the infarcted mouse myocardium.
Smits AM, van Laake LW, den Ouden K, Schreurs C, Szuhai K, van Echteld CJ, Mummery CL, Doevendans PA, Goumans MJ
Cardiovascular research (2009) 833: 527-35. 311 011 IHC
Highly enriched cardiomyocytes from human embryonic stem cells.
Xu XQ, Zweigerdt R, Soo SY, Ngoh ZX, Tham SC, Wang ST, Graichen R, Davidson B, Colman A, Sun W
Cytotherapy (2008) 104: 376-89. 311 011 ICC, IHC
Attachment and growth of human embryonic stem cells on microcarriers.
Phillips BW, Horne R, Lay TS, Rust WL, Teck TT, Crook JM
Journal of biotechnology (2008) 1381-2: 24-32. 311 011 IHC
Efficient expansion of clinical-grade human fibroblasts on microcarriers: cells suitable for ex vivo expansion of clinical-grade hESCs.
Phillips BW, Lim RY, Tan TT, Rust WL, Crook JM
Journal of biotechnology (2008) 1341-2: 79-87. 311 011 IHC
Human embryonic stem cell-derived cardiomyocytes survive and mature in the mouse heart and transiently improve function after myocardial infarction.
van Laake LW, Passier R, Monshouwer-Kloots J, Verkleij AJ, Lips DJ, Freund C, den Ouden K, Ward-van Oostwaard D, Korving J, Tertoolen LG, van Echteld CJ, et al.
Stem cell research (2007) 11: 9-24. 311 011 IHC
Survival and maturation of human embryonic stem cell-derived cardiomyocytes in rat hearts.
Dai W, Field LJ, Rubart M, Reuter S, Hale SL, Zweigerdt R, Graichen RE, Kay GL, Jyrala AJ, Colman A, Davidson BP, et al.
Journal of molecular and cellular cardiology (2007) 434: 504-16. 311 011 IHC
Phosphorylation and translocation of heat shock protein 27 and alphaB-crystallin in human myocardium after cardioplegia and cardiopulmonary bypass.
Clements RT, Sodha NR, Feng J, Mieno S, Boodhwani M, Ramlawi B, Bianchi C, Sellke FW
The Journal of thoracic and cardiovascular surgery (2007) 1346: 1461-70. 311 011 WB, IHC; tested species: human
Rat atrial engineered heart tissue: a new in vitro model to study atrial biology.
Krause J, Löser A, Lemoine MD, Christ T, Scherschel K, Meyer C, Blankenberg S, Zeller T, Eschenhagen T, Stenzig J
Basic research in cardiology (2018) 1135: 41. 311 011 IHC-P; tested species: rat
Optical redox imaging to screen synthetic hydrogels for stem cell-derived cardiomyocyte differentiation and maturation.
Desa DE, Amitrano MJ, Murphy WL, Skala MC
Biophotonics discovery (2024) 11: . 311 011 FACS; tested species: human
LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells.
Song MH, Choi SC, Noh JM, Joo HJ, Park CY, Cha JJ, Ahn TH, Ko TH, Choi JI, Na JE, Rhyu IJ, et al.
Biomaterials (2021) 278: 121133. 311 011 WB, ICC, FACS; tested species: human
Continuous WNT Control Enables Advanced hPSC Cardiac Processing and Prognostic Surface Marker Identification in Chemically Defined Suspension Culture.
Halloin C, Schwanke K, Löbel W, Franke A, Szepes M, Biswanath S, Wunderlich S, Merkert S, Weber N, Osten F, de la Roche J, et al.
Stem cell reports (2019) 132: 366-379. 311 011 ICC, FACS; tested species: human
Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes.
Wiencierz AM, Kernbach M, Ecklebe J, Monnerat G, Tomiuk S, Raulf A, Christalla P, Malan D, Hesse M, Bosio A, Fleischmann BK, et al.
PloS one (2015) 1011: e0143538. 311 011 FACS
Induced pluripotent stem cells as a new strategy for cardiac regeneration and disease modeling.
Iglesias-García O, Pelacho B, Prósper F
Journal of molecular and cellular cardiology (2013) 62: 43-50. 311 011 FACS; tested species: human
Simultaneous voltage and calcium mapping of genetically purified human induced pluripotent stem cell-derived cardiac myocyte monolayers.
Lee P, Klos M, Bollensdorff C, Hou L, Ewart P, Kamp TJ, Zhang J, Bizy A, Guerrero-Serna G, Kohl P, Jalife J, et al.
Circulation research (2012) 11012: 1556-63. 311 011 ICC, FACS
Cat. No.: 311 011
Amount: 100 µg
Price: $430.00
High-throughput longitudinal electrophysiology screening of mature chamber-specific hiPSC-CMs using optical mapping.
Allan A, Creech J, Hausner C, Krajcarski P, Gunawan B, Poulin N, Kozlowski P, Clark CW, Dow R, Saraithong P, Mair DB, et al.
iScience (2023) 267: 107142. 311 011 WB; tested species: human
JAK2 as a surface marker for enrichment of human pluripotent stem cells-derived ventricular cardiomyocytes.
Liew LC, Poh BM, An O, Ho BX, Lim CYY, Pang JKS, Beh LY, Yang HH, Soh BS
Stem cell research & therapy (2023) 141: 367. 311 011 WB, ICC; tested species: human
Myosin essential light chain 1sa decelerates actin and thin filament gliding on β-myosin molecules.
Osten J, Mohebbi M, Uta P, Matinmehr F, Wang T, Kraft T, Amrute-Nayak M, Scholz T
The Journal of general physiology (2022) 15410: . 311 011 WB; tested species: rabbit
LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells.
Song MH, Choi SC, Noh JM, Joo HJ, Park CY, Cha JJ, Ahn TH, Ko TH, Choi JI, Na JE, Rhyu IJ, et al.
Biomaterials (2021) 278: 121133. 311 011 WB, ICC, FACS; tested species: human
Phosphorylation and translocation of heat shock protein 27 and alphaB-crystallin in human myocardium after cardioplegia and cardiopulmonary bypass.
Clements RT, Sodha NR, Feng J, Mieno S, Boodhwani M, Ramlawi B, Bianchi C, Sellke FW
The Journal of thoracic and cardiovascular surgery (2007) 1346: 1461-70. 311 011 WB, IHC; tested species: human
Immune response caused by M1 macrophages elicits atrial fibrillation-like phenotypes in coculture model with isogenic hiPSC-derived cardiomyocytes.
Hutschalik T, Özgül O, Casini M, Szabó B, Peyronnet R, Bártulos Ó, Argenziano M, Schotten U, Matsa E
Stem cell research & therapy (2024) 151: 280. 311 011 ICC; tested species: human
CD151 expression marks atrial- and ventricular- differentiation from human induced pluripotent stem cells.
Nakanishi-Koakutsu M, Miki K, Naka Y, Sasaki M, Wakimizu T, Napier SC, Okubo C, Narita M, Nishikawa M, Hata R, Chonabayashi K, et al.
Communications biology (2024) 71: 231. 311 011 ICC; tested species: human
JAK2 as a surface marker for enrichment of human pluripotent stem cells-derived ventricular cardiomyocytes.
Liew LC, Poh BM, An O, Ho BX, Lim CYY, Pang JKS, Beh LY, Yang HH, Soh BS
Stem cell research & therapy (2023) 141: 367. 311 011 WB, ICC; tested species: human
Establishment of a protocol to administer immunosuppressive drugs for iPS cell-derived cardiomyocyte patch transplantation in a rat myocardial infarction model.
Ito E, Kawamura A, Kawamura T, Takeda M, Harada A, Mochizuki-Oda N, Sawa Y, Miyagawa S
Scientific reports (2023) 131: 10530. 311 011 ICC; tested species: human
Temporal Control of the WNT Signaling Pathway During Cardiac Differentiation Impacts Upon the Maturation State of Human Pluripotent Stem Cell Derived Cardiomyocytes.
Tsoi C, Deng R, Kwok M, Yan B, Lee C, Li HS, Ma CHY, Luo R, Leung KT, Chan GC, Chow LM, et al.
Frontiers in molecular biosciences (2022) 9: 714008. 311 011 ICC; tested species: human
Ultraefficient extracellular vesicle-guided direct reprogramming of fibroblasts into functional cardiomyocytes.
Kim H, Song BW, Park SJ, Choi SW, Moon H, Hwang KC, Kang SW, Moon SH, Yang Y, Kwon IC, Kim SH, et al.
Science advances (2022) 88: eabj6621. 311 011 ICC; tested species: mouse
An iPS-derived in vitro model of human atrial conduction.
Biendarra-Tiegs SM, Yechikov S, Shergill B, Brumback B, Takahashi K, Shirure VS, Gonzalez RE, Houshmand L, Zhong D, Weng KC, Silva J, et al.
Physiological reports (2022) 1018: e15407. 311 011 ICC; tested species: human
LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells.
Song MH, Choi SC, Noh JM, Joo HJ, Park CY, Cha JJ, Ahn TH, Ko TH, Choi JI, Na JE, Rhyu IJ, et al.
Biomaterials (2021) 278: 121133. 311 011 WB, ICC, FACS; tested species: human
Chamber-Specific Protein Expression during Direct Cardiac Reprogramming.
Zhang Z, Villalpando J, Zhang W, Nam YJ
Cells (2021) 106: . 311 011 ICC; tested species: mouse
Cell states beyond transcriptomics: Integrating structural organization and gene expression in hiPSC-derived cardiomyocytes.
Gerbin KA, Grancharova T, Donovan-Maiye RM, Hendershott MC, Anderson HG, Brown JM, Chen J, Dinh SQ, Gehring JL, Johnson GR, Lee H, et al.
Cell systems (2021) 126: 670-687.e10. 311 011 ICC; tested species: human
Spatial Stem Cell Fate Engineering via Facile Morphogen Localization.
Jin G, Floy ME, Simmons AD, Arthur MM, Palecek SP
Advanced healthcare materials (2021) 1021: e2100995. 311 011 ICC; tested species: human
Modeling Hypoxic Stress In Vitro Using Human Embryonic Stem Cells Derived Cardiomyocytes Matured by FGF4 and Ascorbic Acid Treatment.
Choi SC, Seo HR, Cui LH, Song MH, Noh JM, Kim KS, Choi JH, Kim JH, Park CY, Joo HJ, Hong SJ, et al.
Cells (2021) 1010: . 311 011 ICC; tested species: human
Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells.
Fukushima H, Yoshioka M, Kawatou M, López-Dávila V, Takeda M, Kanda Y, Sekino Y, Yoshida Y, Yamashita JK
PloS one (2020) 1511: e0241287. 311 011 ICC; tested species: human
3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells.
Branco MA, Dias TP, Cotovio JP, Rodrigues CAV, Fernandes TG, Cabral JMS, Diogo MM
Methods in molecular biology (Clifton, N.J.) (2020) : . 311 011 ICC; tested species: human
Continuous WNT Control Enables Advanced hPSC Cardiac Processing and Prognostic Surface Marker Identification in Chemically Defined Suspension Culture.
Halloin C, Schwanke K, Löbel W, Franke A, Szepes M, Biswanath S, Wunderlich S, Merkert S, Weber N, Osten F, de la Roche J, et al.
Stem cell reports (2019) 132: 366-379. 311 011 ICC, FACS; tested species: human
Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity.
Atmanli A, Hu D, Deiman FE, van de Vrugt AM, Black LD, Domian I
eLife (2019) 8: . 311 011 ICC; tested species: human
Generation of a Urine-Derived Ips Cell Line from a Patient with a Ventricular Septal Defect and Heart Failure and the Robust Differentiation of These Cells to Cardiomyocytes via Small Molecules.
Cao Y, Xu J, Wen J, Ma X, Liu F, Li Y, Chen W, Sun L, Wu Y, Li S, Li J, et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2018) 502: 538-551. 311 011 ICC; tested species: mouse
Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes.
Lin Y, Linask KL, Mallon B, Johnson K, Klein M, Beers J, Xie W, Du Y, Liu C, Lai Y, Zou J, et al.
Stem cells translational medicine (2017) 62: 527-538. 311 011 ICC; tested species: human
Use of Human Pluripotent Stem Cell Derived-Cardiomyocytes to Study Drug-Induced Cardiotoxicity.
Maillet A, Tan KP, Brunham LR
Current protocols in toxicology (2017) 73: 22.5.1-22.5.22. 311 011 ICC; tested species: human
Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos.
Lalit PA, Rodriguez AM, Downs KM, Kamp TJ
Nature protocols (2017) 125: 1029-1054. 311 011 ICC
Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells.
Pei F, Jiang J, Bai S, Cao H, Tian L, Zhao Y, Yang C, Dong H, Ma Y
Stem cell research (2017) 19: 94-103. 311 011 ICC
Generation of clinical-grade functional cardiomyocytes from human embryonic stem cells in chemically defined conditions.
Tan Y, Han P, Gu Q, Chen G, Wang L, Ma R, Wu J, Feng C, Zhang Y, Wang L, Hu B, et al.
Journal of tissue engineering and regenerative medicine (2016) : . 311 011 ICC; tested species: human
Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.
Dorn T, Goedel A, Lam JT, Haas J, Tian Q, Herrmann F, Bundschu K, Dobreva G, Schiemann M, Dirschinger R, Guo Y, et al.
Stem cells (Dayton, Ohio) (2015) 334: 1113-29. 311 011 ICC, IHC
Fibroblast Growth Factors and Vascular Endothelial Growth Factor Promote Cardiac Reprogramming under Defined Conditions.
Yamakawa H, Muraoka N, Miyamoto K, Sadahiro T, Isomi M, Haginiwa S, Kojima H, Umei T, Akiyama M, Kuishi Y, Kurokawa J, et al.
Stem cell reports (2015) 56: 1128-1142. 311 011 ICC
Cardiac atrial appendage stem cells engraft and differentiate into cardiomyocytes in vivo: A new tool for cardiac repair after MI.
Fanton Y, Robic B, Rummens JL, Daniëls A, Windmolders S, Willems L, Jamaer L, Dubois J, Bijnens E, Heuts N, Notelaers K, et al.
International journal of cardiology (2015) 201: 10-9. 311 011 ICC
Phasic modulation of Wnt signaling enhances cardiac differentiation in human pluripotent stem cells by recapitulating developmental ontogeny.
Mehta A, Ramachandra CJ, Sequiera GL, Sudibyo Y, Nandihalli M, Yong PJ, Koh CH, Shim W
Biochimica et biophysica acta (2014) 184311: 2394-402. 311 011 ICC
Chemically defined generation of human cardiomyocytes.
Burridge PW, Matsa E, Shukla P, Lin ZC, Churko JM, Ebert AD, Lan F, Diecke S, Huber B, Mordwinkin NM, Plews JR, et al.
Nature methods (2014) 118: 855-60. 311 011 ICC
Dantrolene rescues arrhythmogenic RYR2 defect in a patient-specific stem cell model of catecholaminergic polymorphic ventricular tachycardia.
Jung CB, Moretti A, Mederos y Schnitzler M, Iop L, Storch U, Bellin M, Dorn T, Ruppenthal S, Pfeiffer S, Goedel A, Dirschinger RJ, et al.
EMBO molecular medicine (2012) 43: 180-91. 311 011 ICC; tested species: human
Simultaneous voltage and calcium mapping of genetically purified human induced pluripotent stem cell-derived cardiac myocyte monolayers.
Lee P, Klos M, Bollensdorff C, Hou L, Ewart P, Kamp TJ, Zhang J, Bizy A, Guerrero-Serna G, Kohl P, Jalife J, et al.
Circulation research (2012) 11012: 1556-63. 311 011 ICC, FACS
Tbx5 overexpression favors a first heart field lineage in murine embryonic stem cells and in Xenopus laevis embryos.
Herrmann F, Bundschu K, Kühl SJ, Kühl M
Developmental dynamics : an official publication of the American Association of Anatomists (2011) 24012: 2634-45. 311 011 ICC
NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.
Elliott DA, Braam SR, Koutsis K, Ng ES, Jenny R, Lagerqvist EL, Biben C, Hatzistavrou T, Hirst CE, Yu QC, Skelton RJ, et al.
Nature methods (2011) 812: 1037-40. 311 011 ICC
Cardiomyocyte enrichment from human embryonic stem cell cultures by selection of ALCAM surface expression.
Rust W, Balakrishnan T, Zweigerdt R
Regenerative medicine (2009) 42: 225-37. 311 011 ICC
Functional cardiomyocytes derived from human induced pluripotent stem cells.
Zhang J, Wilson GF, Soerens AG, Koonce CH, Yu J, Palecek SP, Thomson JA, Kamp TJ
Circulation research (2009) 1044: e30-41. 311 011 ICC
Highly enriched cardiomyocytes from human embryonic stem cells.
Xu XQ, Zweigerdt R, Soo SY, Ngoh ZX, Tham SC, Wang ST, Graichen R, Davidson B, Colman A, Sun W
Cytotherapy (2008) 104: 376-89. 311 011 ICC, IHC
Cardiomyocytes from human and mouse embryonic stem cells.
Mummery C, van der Heyden MA, de Boer TP, Passier R, Ward D, van den Brink S, van Rooijen M, van de Stolpe A
Methods in molecular medicine (2007) 140: 249-72. 311 011 ICC; tested species: human,mouse
Scalable production of homogeneous cardiac organoids derived from human pluripotent stem cells.
Moriwaki T, Tani H, Haga K, Morita-Umei Y, Soma Y, Umei TC, Sekine O, Takatsuna K, Kishino Y, Kanazawa H, Fujita J, et al.
Cell reports methods (2023) 312: 100666. 311 011 IHC; tested species: human
Development of In Vitro Drug-Induced Cardiotoxicity Assay by Using Three-Dimensional Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells.
Takeda M, Miyagawa S, Fukushima S, Saito A, Ito E, Harada A, Matsuura R, Iseoka H, Sougawa N, Mochizuki-Oda N, Matsusaki M, et al.
Tissue engineering. Part C, Methods (2018) 241: 56-67. 311 011 IHC; tested species: stem cells
Meticulous optimization of cardiomyocyte yields in a 3-stage continuous integrated agitation bioprocess.
Ting S, Lam A, Tong G, Chen A, Wei H, Wu J, Lam YN, Reuveny S, Oh S
Stem cell research (2018) 31: 161-173. 311 011 IHC; tested species: human
Human Embryonic Stem Cell-Derived Cardiomyocytes Self-Arrange with Areas of Different Subtypes During Differentiation.
Vestergaard ML, Grubb S, Koefoed K, Anderson-Jenkins Z, Grunnet-Lauridsen K, Calloe K, Clausen C, Christensen ST, Møllgård K, Andersen CY
Stem cells and development (2017) 2621: 1566-1577. 311 011 IHC; tested species: human
Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.
Dorn T, Goedel A, Lam JT, Haas J, Tian Q, Herrmann F, Bundschu K, Dobreva G, Schiemann M, Dirschinger R, Guo Y, et al.
Stem cells (Dayton, Ohio) (2015) 334: 1113-29. 311 011 ICC, IHC
Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications.
Naaijkens BA, Niessen HW, Prins HJ, Krijnen PA, Kokhuis TJ, de Jong N, van Hinsbergh VW, Kamp O, Helder MN, Musters RJ, van Dijk A, et al.
Cell and tissue research (2012) 3481: 119-30. 311 011 IHC
Human cardiomyocyte progenitor cell transplantation preserves long-term function of the infarcted mouse myocardium.
Smits AM, van Laake LW, den Ouden K, Schreurs C, Szuhai K, van Echteld CJ, Mummery CL, Doevendans PA, Goumans MJ
Cardiovascular research (2009) 833: 527-35. 311 011 IHC
Highly enriched cardiomyocytes from human embryonic stem cells.
Xu XQ, Zweigerdt R, Soo SY, Ngoh ZX, Tham SC, Wang ST, Graichen R, Davidson B, Colman A, Sun W
Cytotherapy (2008) 104: 376-89. 311 011 ICC, IHC
Attachment and growth of human embryonic stem cells on microcarriers.
Phillips BW, Horne R, Lay TS, Rust WL, Teck TT, Crook JM
Journal of biotechnology (2008) 1381-2: 24-32. 311 011 IHC
Efficient expansion of clinical-grade human fibroblasts on microcarriers: cells suitable for ex vivo expansion of clinical-grade hESCs.
Phillips BW, Lim RY, Tan TT, Rust WL, Crook JM
Journal of biotechnology (2008) 1341-2: 79-87. 311 011 IHC
Human embryonic stem cell-derived cardiomyocytes survive and mature in the mouse heart and transiently improve function after myocardial infarction.
van Laake LW, Passier R, Monshouwer-Kloots J, Verkleij AJ, Lips DJ, Freund C, den Ouden K, Ward-van Oostwaard D, Korving J, Tertoolen LG, van Echteld CJ, et al.
Stem cell research (2007) 11: 9-24. 311 011 IHC
Survival and maturation of human embryonic stem cell-derived cardiomyocytes in rat hearts.
Dai W, Field LJ, Rubart M, Reuter S, Hale SL, Zweigerdt R, Graichen RE, Kay GL, Jyrala AJ, Colman A, Davidson BP, et al.
Journal of molecular and cellular cardiology (2007) 434: 504-16. 311 011 IHC
Phosphorylation and translocation of heat shock protein 27 and alphaB-crystallin in human myocardium after cardioplegia and cardiopulmonary bypass.
Clements RT, Sodha NR, Feng J, Mieno S, Boodhwani M, Ramlawi B, Bianchi C, Sellke FW
The Journal of thoracic and cardiovascular surgery (2007) 1346: 1461-70. 311 011 WB, IHC; tested species: human
Rat atrial engineered heart tissue: a new in vitro model to study atrial biology.
Krause J, Löser A, Lemoine MD, Christ T, Scherschel K, Meyer C, Blankenberg S, Zeller T, Eschenhagen T, Stenzig J
Basic research in cardiology (2018) 1135: 41. 311 011 IHC-P; tested species: rat
Optical redox imaging to screen synthetic hydrogels for stem cell-derived cardiomyocyte differentiation and maturation.
Desa DE, Amitrano MJ, Murphy WL, Skala MC
Biophotonics discovery (2024) 11: . 311 011 FACS; tested species: human
LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells.
Song MH, Choi SC, Noh JM, Joo HJ, Park CY, Cha JJ, Ahn TH, Ko TH, Choi JI, Na JE, Rhyu IJ, et al.
Biomaterials (2021) 278: 121133. 311 011 WB, ICC, FACS; tested species: human
Continuous WNT Control Enables Advanced hPSC Cardiac Processing and Prognostic Surface Marker Identification in Chemically Defined Suspension Culture.
Halloin C, Schwanke K, Löbel W, Franke A, Szepes M, Biswanath S, Wunderlich S, Merkert S, Weber N, Osten F, de la Roche J, et al.
Stem cell reports (2019) 132: 366-379. 311 011 ICC, FACS; tested species: human
Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes.
Wiencierz AM, Kernbach M, Ecklebe J, Monnerat G, Tomiuk S, Raulf A, Christalla P, Malan D, Hesse M, Bosio A, Fleischmann BK, et al.
PloS one (2015) 1011: e0143538. 311 011 FACS
Induced pluripotent stem cells as a new strategy for cardiac regeneration and disease modeling.
Iglesias-García O, Pelacho B, Prósper F
Journal of molecular and cellular cardiology (2013) 62: 43-50. 311 011 FACS; tested species: human
Simultaneous voltage and calcium mapping of genetically purified human induced pluripotent stem cell-derived cardiac myocyte monolayers.
Lee P, Klos M, Bollensdorff C, Hou L, Ewart P, Kamp TJ, Zhang J, Bizy A, Guerrero-Serna G, Kohl P, Jalife J, et al.
Circulation research (2012) 11012: 1556-63. 311 011 ICC, FACS
WB
ICC
IHC
IHC
IHC-P
IHC-P
Background

During cardiogenesis two major isoforms of myosin light chain 2 are co-expressed in a tightly regulated manner. MLC-2A is only present in the atrium while MLC-2V is exclusively expressed in the ventricle. Knock out studies revealed that the 2A isoform cannot substitute for the 2V variant in the ventricular chamber.
Recently it has been demonstrated that embryonic and adult stem cells can be differentiated into cardiomyocytes which may generate suitable replacements for damaged heart tissue in the future.
This monoclonal antibody is a useful tool to distinguish between ventricle and atrium specific cardiomyocytes.
Other products for this target