Tailor-made Antibodies
and Tools for Life Science

MLC-2A antibody - 311 011

Myosin light chain 2 or MLC-2A is specific for the atrium of the mammalian heart
Mouse monoclonal purified IgG
Cat. No.: 311 011
Amount: 100 µg
Price: $435.00
Cat. No. 311 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: yes (see remarks) gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  
Clone 56F5
Subtype IgG2b (κ light chain)
Immunogen Full-length recombinant human MLC-2A (UniProt Id: Q01449)
Reactivity Reacts with: human (Q01449), rat, mouse (Q9QVP4).
No signal: chicken.
Other species not tested yet.
Specificity Specific for MLC-2A, no cross-reactivity to MLC-2V.
Matching control protein/peptide 311-0P
Remarks

ICC: Methanol fixation gives stronger signals.

Data sheet 311_011.pdf

References for MLC-2A - 311 011

High-throughput longitudinal electrophysiology screening of mature chamber-specific hiPSC-CMs using optical mapping.
Allan A, Creech J, Hausner C, Krajcarski P, Gunawan B, Poulin N, Kozlowski P, Clark CW, Dow R, Saraithong P, Mair DB, et al.
iScience (2023) 267: 107142. 311 011 WB; tested species: human
JAK2 as a surface marker for enrichment of human pluripotent stem cells-derived ventricular cardiomyocytes.
Liew LC, Poh BM, An O, Ho BX, Lim CYY, Pang JKS, Beh LY, Yang HH, Soh BS
Stem cell research & therapy (2023) 141: 367. 311 011 WB, ICC; tested species: human
Myosin essential light chain 1sa decelerates actin and thin filament gliding on β-myosin molecules.
Osten J, Mohebbi M, Uta P, Matinmehr F, Wang T, Kraft T, Amrute-Nayak M, Scholz T
The Journal of general physiology (2022) 15410: . 311 011 WB; tested species: rabbit
LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells.
Song MH, Choi SC, Noh JM, Joo HJ, Park CY, Cha JJ, Ahn TH, Ko TH, Choi JI, Na JE, Rhyu IJ, et al.
Biomaterials (2021) 278: 121133. 311 011 WB, ICC, FACS; tested species: human
Phosphorylation and translocation of heat shock protein 27 and alphaB-crystallin in human myocardium after cardioplegia and cardiopulmonary bypass.
Clements RT, Sodha NR, Feng J, Mieno S, Boodhwani M, Ramlawi B, Bianchi C, Sellke FW
The Journal of thoracic and cardiovascular surgery (2007) 1346: 1461-70. 311 011 WB, IHC; tested species: human
Cat. No.: 311 011
Amount: 100 µg
Price: $435.00