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Home > Products > 310 111AT647N

MLC-2V antibody - 310 111AT647N

Myosin light chain 2V or MLC-2V is specific for the ventricle of the mammalian heart
Mouse monoclonal purified IgG
Cat. No.: 310 111AT647N
Amount: 100 µg
Price: $465.00
Cat. No. 310 111AT647N 100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO® 647N.

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used.

ATTO® 488: λex 500 nm / λem 520 nm
ATTO® 550: λex 554 nm / λem 576 nm
ATTO® 565: λex 564 nm / λem 590 nm
ATTO® 594: λex 603 nm / λem 626 nm
ATTO® 647N: λex 646 nm / λem 664 nm
 

ATTO®488, ATTO®565, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®488, ATTO®565 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH.
For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: yes (see remarks)
IHC: yes
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label ATTO 647N
Clone 330G5
Subtype IgG2a (κ light chain)
Immunogen Full-length recombinant human MLC-2V protein (UniProt Id: P10916)
Epitop AA 105 to 111 from human MLC-2V (UniProt Id: P10916)
Reactivity Reacts with: human (P10916), rat (P08733), mouse (P51667), pig, chicken.
Other species not tested yet.
Specificity Specific for MLC-2V, no cross-reactivity to MLC-2A.
Remarks

ICC: Methanol fixation gives stronger signals.
Data sheet 310_111at647n.pdf

References for MLC-2V - 310 111AT647N

Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.
Churko JM, Garg P, Treutlein B, Venkatasubramanian M, Wu H, Lee J, Wessells QN, Chen SY, Chen WY, Chetal K, Mantalas G, et al.
Nature communications (2018) 91: 4906. 310 111AT647N ICC; tested species: human
Cat. No.: 310 111AT647N
Amount: 100 µg
Price: $465.00
Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.
Churko JM, Garg P, Treutlein B, Venkatasubramanian M, Wu H, Lee J, Wessells QN, Chen SY, Chen WY, Chetal K, Mantalas G, et al.
Nature communications (2018) 91: 4906. 310 111AT647N ICC; tested species: human
IHC-P
Background

During cardiogenesis two major isoforms of myosin light chain 2 are co-expressed in a tightly regulated manner. MLC-2V is only present in the ventricle while MLC-2A is exclusively expressed in the atrium. Knock out studies revealed that the 2A isoform cannot substitute for the 2V variant in the ventricular chamber.
Recently it has been demonstrated that embryonic and adult stem cells can be differentiated into cardiomyocytes which may generate suitable replacements for damaged heart tissue in the future.
These antibodies are useful tools to distinguish between ventricle and atrium specific cardiomyocytes.
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