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Home > Products > 310 003

MLC-2V antibody - 310 003

Myosin light chain 2V or MLC-2V is specific for the ventricle of the mammalian heart
Rabbit polyclonal purified antibody
Cat. No.: 310 003
Amount: 50 µg
Price: $375.00
Cat. No. 310 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: yes
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 104 to 118 from mouse MLC-2V (UniProt Id: P51667)
Reactivity Reacts with: human (P10916), rat (P08733), mouse (P51667), pig, chicken.
Other species not tested yet.
Specificity Specific for MLC-2V, no cross-reactivity to MLC-2A.
Matching control protein/peptide 310-1P
Data sheet 310_003.pdf

References for MLC-2V - 310 003

Cardiac atrial appendage stem cells engraft and differentiate into cardiomyocytes in vivo: A new tool for cardiac repair after MI.
Fanton Y, Robic B, Rummens JL, Daniëls A, Windmolders S, Willems L, Jamaer L, Dubois J, Bijnens E, Heuts N, Notelaers K, et al.
International journal of cardiology (2015) 201: 10-9. 310 003 ICC
Single-cell transcriptomics provides insights into hypertrophic cardiomyopathy.
Wehrens M, de Leeuw AE, Wright-Clark M, Eding JEC, Boogerd CJ, Molenaar B, van der Kraak PH, Kuster DWD, van der Velden J, Michels M, Vink A, et al.
Cell reports (2022) 396: 110809. 310 003 IHC; tested species: human
A novel 3D label-free monitoring system of hES-derived cardiomyocyte clusters: a step forward to in vitro cardiotoxicity testing.
Jahnke HG, Steel D, Fleischer S, Seidel D, Kurz R, Vinz S, Dahlenborg K, Sartipy P, Robitzki AA
PloS one (2013) 87: e68971. 310 003 IHC
Cat. No.: 310 003
Amount: 50 µg
Price: $375.00
Cardiac atrial appendage stem cells engraft and differentiate into cardiomyocytes in vivo: A new tool for cardiac repair after MI.
Fanton Y, Robic B, Rummens JL, Daniëls A, Windmolders S, Willems L, Jamaer L, Dubois J, Bijnens E, Heuts N, Notelaers K, et al.
International journal of cardiology (2015) 201: 10-9. 310 003 ICC
Single-cell transcriptomics provides insights into hypertrophic cardiomyopathy.
Wehrens M, de Leeuw AE, Wright-Clark M, Eding JEC, Boogerd CJ, Molenaar B, van der Kraak PH, Kuster DWD, van der Velden J, Michels M, Vink A, et al.
Cell reports (2022) 396: 110809. 310 003 IHC; tested species: human
A novel 3D label-free monitoring system of hES-derived cardiomyocyte clusters: a step forward to in vitro cardiotoxicity testing.
Jahnke HG, Steel D, Fleischer S, Seidel D, Kurz R, Vinz S, Dahlenborg K, Sartipy P, Robitzki AA
PloS one (2013) 87: e68971. 310 003 IHC
WB
IHC
IHC
IHC-P
Background

During cardiogenesis two major isoforms of myosin light chain 2 are co-expressed in a tightly regulated manner. MLC-2V is only present in the ventricle while MLC-2A is exclusively expressed in the atrium. Knock out studies revealed that the 2A isoform cannot substitute for the 2V variant in the ventricular chamber.
Recently it has been demonstrated that embryonic and adult stem cells can be differentiated into cardiomyocytes which may generate suitable replacements for damaged heart tissue in the future.
These antibodies are useful tools to distinguish between ventricle and atrium specific cardiomyocytes.
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