Cat. No.: 310-2P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 310-2P |
100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized! |
| Applications |
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| Immunogen | Synthetic peptide corresponding to AA 3 to 14 from human MLC-2V (UniProt Id: P10916) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 310 203 |
| Remarks |
This control peptide consists of the synthetic peptide (aa 3 - 14 of human MLC-2V) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 310-2p.pdf |
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During cardiogenesis two major isoforms of myosin light chain 2 are co-expressed in a tightly regulated manner. MLC-2V is only present in the ventricle while MLC-2A is exclusively expressed in the atrium. Knock out studies revealed that the 2A isoform cannot substitute for the 2V variant in the ventricular chamber.
Recently it has been demonstrated that embryonic and adult stem cells can be differentiated into cardiomyocytes which may generate suitable replacements for damaged heart tissue in the future.
These antibodies are useful tools to distinguish between ventricle and atrium specific cardiomyocytes.