Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 309 011

Sra1 antibody - 309 011

Sra1 is involved in the initiation of actin polymerization
Mouse monoclonal purified IgG
Cat. No.: 309 011
Amount: 100 µg
Price: $415.00
Cat. No. 309 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 2000 (AP staining) gallery  
IP: yes
ICC: not recommended
IHC: not recommended
IHC-P: not tested yet
ELISA: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone 30A4
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 519 to 556 from mouse Sra1 (UniProt Id: Q7TMB8)
Reactivity Reacts with: human (Q7L576), rat, mouse (Q7TMB8).
Other species not tested yet.
Specificity Specific for Sra 1 but may cross-react with CYFIP 2/PIR 121 due to high sequence homology.
Data sheet 309_011.pdf

References for Sra1 - 309 011

WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration.
Xie MJ, Yagi H, Kuroda K, Wang CC, Komada M, Zhao H, Sakakibara A, Miyata T, Nagata K, Oka Y, Iguchi T, et al.
Cerebral cortex (New York, N.Y. : 1991) (2013) 236: 1410-23. 309 011 WB; tested species: mouse
Haploinsufficiency of Cyfip1 produces fragile X-like phenotypes in mice.
Bozdagi O, Sakurai T, Dorr N, Pilorge M, Takahashi N, Buxbaum JD
PloS one (2012) 78: e42422. 309 011 WB; tested species: mouse
Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes.
Steffen A, Faix J, Resch GP, Linkner J, Wehland J, Small JV, Rottner K, Stradal TE
Molecular biology of the cell (2006) 176: 2581-91. 309 011 WB
Cat. No.: 309 011
Amount: 100 µg
Price: $415.00
WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration.
Xie MJ, Yagi H, Kuroda K, Wang CC, Komada M, Zhao H, Sakakibara A, Miyata T, Nagata K, Oka Y, Iguchi T, et al.
Cerebral cortex (New York, N.Y. : 1991) (2013) 236: 1410-23. 309 011 WB; tested species: mouse
Haploinsufficiency of Cyfip1 produces fragile X-like phenotypes in mice.
Bozdagi O, Sakurai T, Dorr N, Pilorge M, Takahashi N, Buxbaum JD
PloS one (2012) 78: e42422. 309 011 WB; tested species: mouse
Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes.
Steffen A, Faix J, Resch GP, Linkner J, Wehland J, Small JV, Rottner K, Stradal TE
Molecular biology of the cell (2006) 176: 2581-91. 309 011 WB
WB
Background

The formation of cellular projections like lamellipodia and ruffles is accompanied by de novo actin nucleation and polymerization of actin filaments. Several protein factors like the Arp 2/3 complex, WASP/Scar and small GTPases of the Rho family have been shown to participate in this process.
The Arp 2/3 complex is activated by GTP-loaded Rac 1 via WAVE proteins. The Specifically Rac-associated protein or Sra 1, also referred to as cytoplasmic FMR 1 interacting protein 1, has been shown to interact with WAVE 2 and other proteins like Abi 1 at the tips of membrane protrusions.