Synaptic Systems - Bovine Profilin

Cat. No.: 308 011

Amount: 100 µg

Price: $415.00

Cat. No. 308 011	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: yes IHC: yes IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	2H11
Subtype	IgG1 (λ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 140 from bovine Profilin1 (UniProt Id: P02584)
Reactivity	Reacts with: human (P07737), cow, rabbit, Guinea pig, opossum. No signal: mouse (P62962). Other species not tested yet.
Data sheet	308_011.pdf

References for Bovine Profilin - 308 011

Suppression of tumorigenicity in breast cancer cells by the microfilament protein profilin 1.
Janke J, Schlüter K, Jandrig B, Theile M, Kölble K, Arnold W, Grinstein E, Schwartz A, Estevéz-Schwarz L, Schlag PM, Jockusch BM, et al.
The Journal of experimental medicine (2000) 19110: 1675-86. 308 011 WB, ICC

Functional characterization of green fluorescent protein-profilin fusion proteins.
Wittenmayer N, Rothkegel M, Jockusch BM, Schlüter K
European journal of biochemistry (2000) 26716: 5247-56. 308 011 WB

Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I.
Schlüter K, Schleicher M, Jockusch BM
Journal of cell science (1998) 111 ( Pt 22): 3261-73. 308 011 WB, ICC

Actin filaments at the leading edge of cancer cells are characterized by a high mobile fraction and turnover regulation by profilin I.
Lorente G, Syriani E, Morales M
PloS one (2014) 91: e85817. 308 011 ICC

A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems.
Giesemann T, Rathke-Hartlieb S, Rothkegel M, Bartsch JW, Buchmeier S, Jockusch BM, Jockusch H
The Journal of biological chemistry (1999) 27453: 37908-14. 308 011 ICC

Complex formation between the postsynaptic scaffolding protein gephyrin, profilin, and Mena: a possible link to the microfilament system.
Giesemann T, Schwarz G, Nawrotzki R, Berhörster K, Rothkegel M, Schlüter K, Schrader N, Schindelin H, Mendel RR, Kirsch J, Jockusch BM, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2003) 2323: 8330-9. 308 011 IHC

Proteomic comparison of nasopharyngeal cancer cell lines C666-1 and NP69 identifies down-regulation of annexin II and beta2-tubulin for nasopharyngeal carcinoma.
Chan CM, Wong SC, Lam MY, Hui EP, Chan JK, Lo ES, Cheuk W, Wong MC, Tsao SW, Chan AT
Archives of pathology & laboratory medicine (2008) 1324: 675-83. 308 011 IHC-P; tested species: human

Cat. No.: 308 011

Amount: 100 µg

Price: $415.00

IHC-P

Background

Profilins are small proteins (14-17 kDa) which are involved in the regulation of the cellular microfilament system. They are associated with highly dynamic microfilament structures present at cellular membranes. Profilins have been found together with lamellipodia, focal adhesions, surface ruffles and on intracellular vesicles and have been shown to interact with different cytoskeleton proteins like actin, gephyrin and the Arp 2/3 complex. Recently a tumor suppression activity has been described for profilin.

1 ) Neuhoff H et al., Eur. J. Neurosci. (2005)
2 ) Wittenmayer N et al., Mol. Biol. Cell (2004)

Protocols

ICC

IHC

IHC-P

Bovine Profilin antibody - 308 011

References for Bovine Profilin - 308 011