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Home > Products > 306 011

p34-Arc antibody - 306 011

p34-Arc is a member of the seven protein complex required for actin nucleation
Mouse monoclonal purified IgG
Cat. No.: 306 011
Amount: 100 µg
Price: $415.00
Cat. No. 306 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: yes gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 334D4
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 300 from bovine p34-Arc (UniProt Id: Q3MHR7)
Epitop AA 229 to 240 from bovine p34-Arc (UniProt Id: Q3MHR7)
Reactivity Reacts with: human (O15144), cow (Q3MHR7), pig, rat (P85970), mouse (Q9CVB6).
Other species not tested yet.
Data sheet 306_011.pdf

References for p34-Arc - 306 011

Presynapses contain distinct actin nanostructures.
Bingham D, Jakobs CE, Wernert F, Boroni-Rueda F, Jullien N, Schentarra EM, Friedl K, Da Costa Moura J, van Bommel DM, Caillol G, Ogawa Y, et al.
The Journal of cell biology (2023) 22210: . 306 011 ICC; tested species: rat
Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics.
Dubois T, Paléotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P
Nature cell biology (2005) 74: 353-64. 306 011 ICC; tested species: human
Cat. No.: 306 011
Amount: 100 µg
Price: $415.00
Presynapses contain distinct actin nanostructures.
Bingham D, Jakobs CE, Wernert F, Boroni-Rueda F, Jullien N, Schentarra EM, Friedl K, Da Costa Moura J, van Bommel DM, Caillol G, Ogawa Y, et al.
The Journal of cell biology (2023) 22210: . 306 011 ICC; tested species: rat
Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics.
Dubois T, Paléotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P
Nature cell biology (2005) 74: 353-64. 306 011 ICC; tested species: human
ICC
ICC
Background
Actin polymerization is a necessary event for cell motility. Spontanous actin oligomerization is slow at given monomeric actin concentrations in cells. The Arp 2/3 complex which is about 220 kDa in size has turned out to initiate the polymerization of new actin filaments. This complex consists of two actin like proteins Arp2 and Arp3 and five additional proteins: p16-Arc (ArpC5), p20-Arc (ArpC4), p21-Arc (ArpC3), p34-Arc (ArpC2) and p41-Arc (ArpC1). Expression of partial complexes revealed that a heterodimer of p20-Arc and p34-Arc constitutes the core of the complex whereas the remaining subunits are peripherally located.