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Home > Products > 305 011

p16-Arc antibody - 305 011 K.O.

p16-Arc is a member of the seven protein complex required for actin nucleation
Mouse monoclonal purified IgG
Cat. No.: 305 011
Amount: 100 µg
Price: $415.00
Cat. No. 305 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: yes
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 323H3
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 151 from human p16-Arc (UniProt Id: O15511)
Epitop AA 19 to 24 from human p16-Arc (UniProt Id: O15511)
Reactivity Reacts with: human (O15511), rat (Q4KLF8), mouse (Q9CPW4), hamster.
Other species not tested yet.
Specificity K.O. validated PubMed: 37349293
Data sheet 305_011.pdf

References for p16-Arc - 305 011

Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling.
Nunes-Santos CJ, Kuehn H, Boast B, Hwang S, Kuhns DB, Stoddard J, Niemela JE, Fink DL, Pittaluga S, Abu-Asab M, Davies JS, et al.
Nature communications (2023) 141: 3708. 305 011 WB; KO verified; tested species: human
ARPC5 isoforms and their regulation by calcium-calmodulin-N-WASP drive distinct Arp2/3-dependent actin remodeling events in CD4 T cells.
Sadhu L, Tsopoulidis N, Hasanuzzaman M, Laketa V, Way M, Fackler OT
eLife (2023) 12: . 305 011 WB; KD verified; tested species: mouse
MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.
Galloni C, Carra D, Abella JVG, Kjær S, Singaravelu P, Barry DJ, Kogata N, Guérin C, Blanchoin L, Way M
The Journal of cell biology (2021) 2208: . 305 011 WB; tested species: human
The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex.
Fokin AI, David V, Oguievetskaia K, Derivery E, Stone CE, Cao L, Rocques N, Molinie N, Henriot V, Aumont-Nicaise M, Hinckelmann MV, et al.
Science advances (2021) 73: . 305 011 WB; tested species: mouse
Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production.
Chen H, Victor AK, Klein J, Tacer KF, Tai DJ, de Esch C, Nuttle A, Temirov J, Burnett LC, Rosenbaum M, Zhang Y, et al.
JCI insight (2020) 517: . 305 011 WB; tested species: human
Ezrin promotes actin assembly at the phagosome membrane and regulates phago-lysosomal fusion.
Marion S, Hoffmann E, Holzer D, Le Clainche C, Martin M, Sachse M, Ganeva I, Mangeat P, Griffiths G
Traffic (Copenhagen, Denmark) (2011) 124: 421-37. 305 011 WB; tested species: mouse
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 305 011 WB
Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes.
Steffen A, Faix J, Resch GP, Linkner J, Wehland J, Small JV, Rottner K, Stradal TE
Molecular biology of the cell (2006) 176: 2581-91. 305 011 WB
ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.
Fäßler F, Javoor MG, Datler J, Döring H, Hofer FW, Dimchev G, Hodirnau VV, Faix J, Rottner K, Schur FKM
Science advances (2023) 93: eadd6495. 305 011 ICC; tested species: mouse
Microtubules as platforms for assaying actin polymerization in vivo.
Oelkers JM, Vinzenz M, Nemethova M, Jacob S, Lai FP, Block J, Szczodrak M, Kerkhoff E, Backert S, Schlüter K, Stradal TE, et al.
PloS one (2011) 65: e19931. 305 011 ICC
Protein kinase Cdelta and calmodulin regulate epidermal growth factor receptor recycling from early endosomes through Arp2/3 complex and cortactin.
Lladó A, Timpson P, Vilà de Muga S, Moretó J, Pol A, Grewal T, Daly RJ, Enrich C, Tebar F
Molecular biology of the cell (2008) 191: 17-29. 305 011 ICC
Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics.
Dubois T, Paléotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P
Nature cell biology (2005) 74: 353-64. 305 011 ICC; tested species: human
Cat. No.: 305 011
Amount: 100 µg
Price: $415.00
Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling.
Nunes-Santos CJ, Kuehn H, Boast B, Hwang S, Kuhns DB, Stoddard J, Niemela JE, Fink DL, Pittaluga S, Abu-Asab M, Davies JS, et al.
Nature communications (2023) 141: 3708. 305 011 WB; KO verified; tested species: human
ARPC5 isoforms and their regulation by calcium-calmodulin-N-WASP drive distinct Arp2/3-dependent actin remodeling events in CD4 T cells.
Sadhu L, Tsopoulidis N, Hasanuzzaman M, Laketa V, Way M, Fackler OT
eLife (2023) 12: . 305 011 WB; KD verified; tested species: mouse
MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.
Galloni C, Carra D, Abella JVG, Kjær S, Singaravelu P, Barry DJ, Kogata N, Guérin C, Blanchoin L, Way M
The Journal of cell biology (2021) 2208: . 305 011 WB; tested species: human
The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex.
Fokin AI, David V, Oguievetskaia K, Derivery E, Stone CE, Cao L, Rocques N, Molinie N, Henriot V, Aumont-Nicaise M, Hinckelmann MV, et al.
Science advances (2021) 73: . 305 011 WB; tested species: mouse
Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production.
Chen H, Victor AK, Klein J, Tacer KF, Tai DJ, de Esch C, Nuttle A, Temirov J, Burnett LC, Rosenbaum M, Zhang Y, et al.
JCI insight (2020) 517: . 305 011 WB; tested species: human
Ezrin promotes actin assembly at the phagosome membrane and regulates phago-lysosomal fusion.
Marion S, Hoffmann E, Holzer D, Le Clainche C, Martin M, Sachse M, Ganeva I, Mangeat P, Griffiths G
Traffic (Copenhagen, Denmark) (2011) 124: 421-37. 305 011 WB; tested species: mouse
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 305 011 WB
Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes.
Steffen A, Faix J, Resch GP, Linkner J, Wehland J, Small JV, Rottner K, Stradal TE
Molecular biology of the cell (2006) 176: 2581-91. 305 011 WB
ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.
Fäßler F, Javoor MG, Datler J, Döring H, Hofer FW, Dimchev G, Hodirnau VV, Faix J, Rottner K, Schur FKM
Science advances (2023) 93: eadd6495. 305 011 ICC; tested species: mouse
Microtubules as platforms for assaying actin polymerization in vivo.
Oelkers JM, Vinzenz M, Nemethova M, Jacob S, Lai FP, Block J, Szczodrak M, Kerkhoff E, Backert S, Schlüter K, Stradal TE, et al.
PloS one (2011) 65: e19931. 305 011 ICC
Protein kinase Cdelta and calmodulin regulate epidermal growth factor receptor recycling from early endosomes through Arp2/3 complex and cortactin.
Lladó A, Timpson P, Vilà de Muga S, Moretó J, Pol A, Grewal T, Daly RJ, Enrich C, Tebar F
Molecular biology of the cell (2008) 191: 17-29. 305 011 ICC
Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics.
Dubois T, Paléotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P
Nature cell biology (2005) 74: 353-64. 305 011 ICC; tested species: human
WB
Background
Actin polymerization is a necessary event for cell motility. Spontanous actin oligomerization is slow at given monomeric actin concentrations in cells. The Arp 2/3 complex which is about 220 kDa in size has turned out to initiate the polymerization of new actin filaments. This complex consists of two actin like proteins Arp2 and Arp3 and five additional proteins: p16-Arc (ArpC5), p20-Arc (ArpC4), p21-Arc (ArpC3), p34-Arc (ArpC2) and p41-Arc (ArpC1). Expression of partial complexes revealed that a heterodimer of p20-Arc and p34-Arc constitutes the core of the complex whereas the remaining subunits are peripherally located.