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Home > Products > 302 304

beta3-Tubulin antibody - 302 304

beta3-Tubulin also known as TuJ1 is a neuron specific Tubulin
Guinea pig polyclonal antiserum
Cat. No.: 302 304
Amount: 100 µl
Price: $370.00
Cat. No. 302 304 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 (see remarks) gallery  
IHC-P: 1 : 200 up to 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse β3-tubulin (UniProt Id: Q9ERD7)
Reactivity Reacts with: human (Q13509), rat (Q4QRB4), mouse (Q9ERD7).
Other species not tested yet.
Specificity Specific for β3-tubulin.
Matching control protein/peptide 302-3P
Remarks

IHC: For best results we recommend antigen retrieval with pepsin according to Lorincz A & Nusser Z (2008).
Data sheet 302_304.pdf

References for beta3-Tubulin - 302 304

Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 302 304 WB; tested species: rat
Pre-clinical development of AP4B1 gene replacement therapy for hereditary spastic paraplegia type 47.
Wiseman JP, Scarrott JM, Alves-Cruzeiro J, Saffari A, Böger C, Karyka E, Dawes E, Davies AK, Marchi PM, Graves E, Fernandes F, et al.
EMBO molecular medicine (2024) : . 302 304 ICC; tested species: human
Epigenetic modulation through BET bromodomain inhibitors as a novel therapeutic strategy for progranulin-deficient frontotemporal dementia.
Rosenthal ZC, Fass DM, Payne NC, She A, Patnaik D, Hennig KM, Tesla R, Werthmann GC, Guhl C, Reis SA, Wang X, et al.
Scientific reports (2024) 141: 9064. 302 304 ICC; tested species: human
The autism susceptibility kinase, TAOK2, phosphorylates eEF2 and modulates translation.
Henis M, Rücker T, Scharrenberg R, Richter M, Baltussen L, Hong S, Meka DP, Schwanke B, Neelagandan N, Daaboul D, Murtaza N, et al.
Science advances (2024) 1015: eadf7001. 302 304 ICC; tested species: mouse
Generation of two iPSC lines from Mowat-Wilson syndrome patients carrying heterozygous ZEB2 mutations.
Gorrieri G, Tamburro S, Baldassari S, Guerrisi S, Zara F, Ricci E, Maria Cordelli D, Scudieri P, Musante I
Stem cell research (2024) 76: 103333. 302 304 ICC; tested species: human
Direct neuronal reprogramming of NDUFS4 patient cells identifies the unfolded protein response as a novel general reprogramming hurdle.
Sonsalla G, Malpartida AB, Riedemann T, Gusic M, Rusha E, Bulli G, Najas S, Janjic A, Hersbach BA, Smialowski P, Drukker M, et al.
Neuron (2024) : . 302 304 ICC; tested species: human
High-content screening identifies a small molecule that restores AP-4-dependent protein trafficking in neuronal models of AP-4-associated hereditary spastic paraplegia.
Saffari A, Brechmann B, Böger C, Saber WA, Jumo H, Whye D, Wood D, Wahlster L, Alecu JE, Ziegler M, Scheffold M, et al.
Nature communications (2024) 151: 584. 302 304 ICC; tested species: human
Generation of an induced pluripotent stem cell line (IGGi002A) from nasal cells of a cystic fibrosis patient homozygous for the G542X-CFTR mutation.
Dębczyński M, Mojsak D, Tamburro S, Baldassari S, Musante I, Casciaro R, Ciciriello F, Zara F, Scudieri P, Gorrieri G
Stem cell research (2023) 72: 103232. 302 304 ICC; tested species: human
Developmental Pb exposure increases AD risk via altered intracellular Ca2+ homeostasis in hiPSC-derived cortical neurons.
Xie J, Wu S, Szadowski H, Min S, Yang Y, Bowman AB, Rochet JC, Freeman JL, Yuan C
The Journal of biological chemistry (2023) : 105023. 302 304 ICC; tested species: human
Metabolic control of adult neural stem cell self-renewal by the mitochondrial protease YME1L.
Wani GA, Sprenger HG, Ndoci K, Chandragiri S, Acton RJ, Schatton D, Kochan SMV, Sakthivelu V, Jevtic M, Seeger JM, Müller S, et al.
Cell reports (2022) 387: 110370. 302 304 ICC; tested species: mouse
Centrosome-dependent microtubule modifications set the conditions for axon formation.
Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, Ruecker T, et al.
Cell reports (2022) 393: 110686. 302 304 ICC; tested species: rat
AP-4-mediated axonal transport controls endocannabinoid production in neurons.
Davies AK, Alecu JE, Ziegler M, Vasilopoulou CG, Merciai F, Jumo H, Afshar-Saber W, Sahin M, Ebrahimi-Fakhari D, Borner GHH
Nature communications (2022) 131: 1058. 302 304 ICC; tested species: mouse
Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro.
Pigareva Y, Gladkov A, Kolpakov V, Mukhina I, Bukatin A, Kazantsev VB, Pimashkin A
Brain sciences (2021) 116: . 302 304 ICC; tested species: mouse
Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.
Gunaseelan S, Wang Z, Tong VKJ, Ming SWS, Razar RBBA, Srimasorn S, Ong WY, Lim KL, Chua JJE
Human molecular genetics (2021) : . 302 304 ICC; tested species: human
Brain-Derived Neurotrophic Factor (BDNF) Preserves the Functional Integrity of Neural Networks in the β-Amyloidopathy Model in vitro.
Mitroshina EV, Yarkov RS, Mishchenko TA, Krut' VG, Gavrish MS, Epifanova EA, Babaev AA, Vedunova MV
Frontiers in cell and developmental biology (2020) 8: 582. 302 304 ICC; tested species: mouse
Design of Cultured Neuron Networks in vitro with Predefined Connectivity Using Asymmetric Microfluidic Channels.
Gladkov A, Pigareva Y, Kutyina D, Kolpakov V, Bukatin A, Mukhina I, Kazantsev V, Pimashkin A
Scientific reports (2017) 71: 15625. 302 304 ICC; tested species: mouse
Methyl-4-phenylpyridinium (MPP+) differentially affects monoamine release and re-uptake in murine embryonic stem cell-derived dopaminergic and serotonergic neurons.
Martí Y, Matthaeus F, Lau T, Schloss P
Molecular and cellular neurosciences (2017) 83: 37-45. 302 304 ICC; tested species: mouse
mGlu5 Receptors and Relapse to Cocaine-Seeking: The Role of Receptor Trafficking in Postrelapse Extinction Learning Deficits.
Knackstedt LA, Schwendt M
Neural plasticity (2016) 2016: 9312508. 302 304 ICC
Axonal transport and secretion of fibrillar forms of α-synuclein, Aβ42 peptide and HTTExon 1.
Brahic M, Bousset L, Bieri G, Melki R, Gitler AD
Acta neuropathologica (2016) 1314: 539-48. 302 304 ICC
Nongenomic, glucocorticoid receptor-mediated regulation of serotonin transporter cell surface expression in embryonic stem cell derived serotonergic neurons.
Lau T, Heimann F, Bartsch D, Schloss P, Weber T
Neuroscience letters (2013) 554: 115-20. 302 304 ICC; tested species: mouse
Morphological and functional differentiation in BE(2)-M17 human neuroblastoma cells by treatment with Trans-retinoic acid.
Andres D, Keyser BM, Petrali J, Benton B, Hubbard KS, McNutt PM, Ray R
BMC neuroscience (2013) 14: 49. 302 304 ICC
Radiology of inflammatory bowel disease.

Radiologic clinics of North America (1987) 251: 1-237. 302 304 ICC; tested species: human
The RNA-Binding Protein HuR Is Integral to the Function of Nociceptors in Mice and Humans.
Kunder N, de la Peña JB, Lou TF, Chase R, Suresh P, Lawson J, Shukla T, Black B, Campbell ZT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4249: 9129-9141. 302 304 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 302 304 IHC; tested species: mouse
Egr2 overexpression in Schwann cells increases myelination frequency in vitro.
Tammia M, Mi R, Sluch VM, Zhu A, Chung T, Shinn D, Zack DJ, Höke A, Mao HQ
Heliyon (2018) 411: e00982. 302 304 IHC; tested species: rat
Cat. No.: 302 304
Amount: 100 µl
Price: $370.00
Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 302 304 WB; tested species: rat
Pre-clinical development of AP4B1 gene replacement therapy for hereditary spastic paraplegia type 47.
Wiseman JP, Scarrott JM, Alves-Cruzeiro J, Saffari A, Böger C, Karyka E, Dawes E, Davies AK, Marchi PM, Graves E, Fernandes F, et al.
EMBO molecular medicine (2024) : . 302 304 ICC; tested species: human
Epigenetic modulation through BET bromodomain inhibitors as a novel therapeutic strategy for progranulin-deficient frontotemporal dementia.
Rosenthal ZC, Fass DM, Payne NC, She A, Patnaik D, Hennig KM, Tesla R, Werthmann GC, Guhl C, Reis SA, Wang X, et al.
Scientific reports (2024) 141: 9064. 302 304 ICC; tested species: human
The autism susceptibility kinase, TAOK2, phosphorylates eEF2 and modulates translation.
Henis M, Rücker T, Scharrenberg R, Richter M, Baltussen L, Hong S, Meka DP, Schwanke B, Neelagandan N, Daaboul D, Murtaza N, et al.
Science advances (2024) 1015: eadf7001. 302 304 ICC; tested species: mouse
Generation of two iPSC lines from Mowat-Wilson syndrome patients carrying heterozygous ZEB2 mutations.
Gorrieri G, Tamburro S, Baldassari S, Guerrisi S, Zara F, Ricci E, Maria Cordelli D, Scudieri P, Musante I
Stem cell research (2024) 76: 103333. 302 304 ICC; tested species: human
Direct neuronal reprogramming of NDUFS4 patient cells identifies the unfolded protein response as a novel general reprogramming hurdle.
Sonsalla G, Malpartida AB, Riedemann T, Gusic M, Rusha E, Bulli G, Najas S, Janjic A, Hersbach BA, Smialowski P, Drukker M, et al.
Neuron (2024) : . 302 304 ICC; tested species: human
High-content screening identifies a small molecule that restores AP-4-dependent protein trafficking in neuronal models of AP-4-associated hereditary spastic paraplegia.
Saffari A, Brechmann B, Böger C, Saber WA, Jumo H, Whye D, Wood D, Wahlster L, Alecu JE, Ziegler M, Scheffold M, et al.
Nature communications (2024) 151: 584. 302 304 ICC; tested species: human
Generation of an induced pluripotent stem cell line (IGGi002A) from nasal cells of a cystic fibrosis patient homozygous for the G542X-CFTR mutation.
Dębczyński M, Mojsak D, Tamburro S, Baldassari S, Musante I, Casciaro R, Ciciriello F, Zara F, Scudieri P, Gorrieri G
Stem cell research (2023) 72: 103232. 302 304 ICC; tested species: human
Developmental Pb exposure increases AD risk via altered intracellular Ca2+ homeostasis in hiPSC-derived cortical neurons.
Xie J, Wu S, Szadowski H, Min S, Yang Y, Bowman AB, Rochet JC, Freeman JL, Yuan C
The Journal of biological chemistry (2023) : 105023. 302 304 ICC; tested species: human
Metabolic control of adult neural stem cell self-renewal by the mitochondrial protease YME1L.
Wani GA, Sprenger HG, Ndoci K, Chandragiri S, Acton RJ, Schatton D, Kochan SMV, Sakthivelu V, Jevtic M, Seeger JM, Müller S, et al.
Cell reports (2022) 387: 110370. 302 304 ICC; tested species: mouse
Centrosome-dependent microtubule modifications set the conditions for axon formation.
Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, Ruecker T, et al.
Cell reports (2022) 393: 110686. 302 304 ICC; tested species: rat
AP-4-mediated axonal transport controls endocannabinoid production in neurons.
Davies AK, Alecu JE, Ziegler M, Vasilopoulou CG, Merciai F, Jumo H, Afshar-Saber W, Sahin M, Ebrahimi-Fakhari D, Borner GHH
Nature communications (2022) 131: 1058. 302 304 ICC; tested species: mouse
Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro.
Pigareva Y, Gladkov A, Kolpakov V, Mukhina I, Bukatin A, Kazantsev VB, Pimashkin A
Brain sciences (2021) 116: . 302 304 ICC; tested species: mouse
Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.
Gunaseelan S, Wang Z, Tong VKJ, Ming SWS, Razar RBBA, Srimasorn S, Ong WY, Lim KL, Chua JJE
Human molecular genetics (2021) : . 302 304 ICC; tested species: human
Brain-Derived Neurotrophic Factor (BDNF) Preserves the Functional Integrity of Neural Networks in the β-Amyloidopathy Model in vitro.
Mitroshina EV, Yarkov RS, Mishchenko TA, Krut' VG, Gavrish MS, Epifanova EA, Babaev AA, Vedunova MV
Frontiers in cell and developmental biology (2020) 8: 582. 302 304 ICC; tested species: mouse
Design of Cultured Neuron Networks in vitro with Predefined Connectivity Using Asymmetric Microfluidic Channels.
Gladkov A, Pigareva Y, Kutyina D, Kolpakov V, Bukatin A, Mukhina I, Kazantsev V, Pimashkin A
Scientific reports (2017) 71: 15625. 302 304 ICC; tested species: mouse
Methyl-4-phenylpyridinium (MPP+) differentially affects monoamine release and re-uptake in murine embryonic stem cell-derived dopaminergic and serotonergic neurons.
Martí Y, Matthaeus F, Lau T, Schloss P
Molecular and cellular neurosciences (2017) 83: 37-45. 302 304 ICC; tested species: mouse
mGlu5 Receptors and Relapse to Cocaine-Seeking: The Role of Receptor Trafficking in Postrelapse Extinction Learning Deficits.
Knackstedt LA, Schwendt M
Neural plasticity (2016) 2016: 9312508. 302 304 ICC
Axonal transport and secretion of fibrillar forms of α-synuclein, Aβ42 peptide and HTTExon 1.
Brahic M, Bousset L, Bieri G, Melki R, Gitler AD
Acta neuropathologica (2016) 1314: 539-48. 302 304 ICC
Nongenomic, glucocorticoid receptor-mediated regulation of serotonin transporter cell surface expression in embryonic stem cell derived serotonergic neurons.
Lau T, Heimann F, Bartsch D, Schloss P, Weber T
Neuroscience letters (2013) 554: 115-20. 302 304 ICC; tested species: mouse
Morphological and functional differentiation in BE(2)-M17 human neuroblastoma cells by treatment with Trans-retinoic acid.
Andres D, Keyser BM, Petrali J, Benton B, Hubbard KS, McNutt PM, Ray R
BMC neuroscience (2013) 14: 49. 302 304 ICC
Radiology of inflammatory bowel disease.

Radiologic clinics of North America (1987) 251: 1-237. 302 304 ICC; tested species: human
The RNA-Binding Protein HuR Is Integral to the Function of Nociceptors in Mice and Humans.
Kunder N, de la Peña JB, Lou TF, Chase R, Suresh P, Lawson J, Shukla T, Black B, Campbell ZT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4249: 9129-9141. 302 304 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 302 304 IHC; tested species: mouse
Egr2 overexpression in Schwann cells increases myelination frequency in vitro.
Tammia M, Mi R, Sluch VM, Zhu A, Chung T, Shinn D, Zack DJ, Höke A, Mao HQ
Heliyon (2018) 411: e00982. 302 304 IHC; tested species: rat
WB
ICC
IHC
IHC-P
IHC-P
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape (1).
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter (1).
Class III β-tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development (2).
It is widely used as a neuronal marker in normal and neoplastic tissues but has also been reported to be expressed in certain tumours of non-neuronal origin (3).
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