Synaptic Systems - beta3-Tubulin

Cat. No.: 302 304

Amount: 100 µl

Price: $375.00

Cat. No. 302 304	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 (see remarks) gallery IHC-P: 1 : 200 up to 1 : 500 gallery
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse β3-tubulin (UniProt Id: Q9ERD7)
Reactivity	Reacts with: human (Q13509), rat (Q4QRB4), mouse (Q9ERD7). Other species not tested yet.
Specificity	Specific for β3-tubulin.
Matching control protein/peptide	302-3P
Remarks	IHC: For best results we recommend antigen retrieval with pepsin according to Lorincz A & Nusser Z (2008).
Data sheet	302_304.pdf

References for beta3-Tubulin - 302 304

WB (1)
ICC (21)
IHC (3)

Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 302 304 WB; tested species: rat

Pre-clinical development of AP4B1 gene replacement therapy for hereditary spastic paraplegia type 47.
Wiseman JP, Scarrott JM, Alves-Cruzeiro J, Saffari A, Böger C, Karyka E, Dawes E, Davies AK, Marchi PM, Graves E, Fernandes F, et al.
EMBO molecular medicine (2024) : . 302 304 ICC; tested species: human

Epigenetic modulation through BET bromodomain inhibitors as a novel therapeutic strategy for progranulin-deficient frontotemporal dementia.
Rosenthal ZC, Fass DM, Payne NC, She A, Patnaik D, Hennig KM, Tesla R, Werthmann GC, Guhl C, Reis SA, Wang X, et al.
Scientific reports (2024) 141: 9064. 302 304 ICC; tested species: human

The autism susceptibility kinase, TAOK2, phosphorylates eEF2 and modulates translation.
Henis M, Rücker T, Scharrenberg R, Richter M, Baltussen L, Hong S, Meka DP, Schwanke B, Neelagandan N, Daaboul D, Murtaza N, et al.
Science advances (2024) 1015: eadf7001. 302 304 ICC; tested species: mouse

Generation of two iPSC lines from Mowat-Wilson syndrome patients carrying heterozygous ZEB2 mutations.
Gorrieri G, Tamburro S, Baldassari S, Guerrisi S, Zara F, Ricci E, Maria Cordelli D, Scudieri P, Musante I
Stem cell research (2024) 76: 103333. 302 304 ICC; tested species: human

Direct neuronal reprogramming of NDUFS4 patient cells identifies the unfolded protein response as a novel general reprogramming hurdle.
Sonsalla G, Malpartida AB, Riedemann T, Gusic M, Rusha E, Bulli G, Najas S, Janjic A, Hersbach BA, Smialowski P, Drukker M, et al.
Neuron (2024) : . 302 304 ICC; tested species: human

High-content screening identifies a small molecule that restores AP-4-dependent protein trafficking in neuronal models of AP-4-associated hereditary spastic paraplegia.
Saffari A, Brechmann B, Böger C, Saber WA, Jumo H, Whye D, Wood D, Wahlster L, Alecu JE, Ziegler M, Scheffold M, et al.
Nature communications (2024) 151: 584. 302 304 ICC; tested species: human

Generation of an induced pluripotent stem cell line (IGGi002A) from nasal cells of a cystic fibrosis patient homozygous for the G542X-CFTR mutation.
Dębczyński M, Mojsak D, Tamburro S, Baldassari S, Musante I, Casciaro R, Ciciriello F, Zara F, Scudieri P, Gorrieri G
Stem cell research (2023) 72: 103232. 302 304 ICC; tested species: human

Developmental Pb exposure increases AD risk via altered intracellular Ca2+ homeostasis in hiPSC-derived cortical neurons.
Xie J, Wu S, Szadowski H, Min S, Yang Y, Bowman AB, Rochet JC, Freeman JL, Yuan C
The Journal of biological chemistry (2023) : 105023. 302 304 ICC; tested species: human

Metabolic control of adult neural stem cell self-renewal by the mitochondrial protease YME1L.
Wani GA, Sprenger HG, Ndoci K, Chandragiri S, Acton RJ, Schatton D, Kochan SMV, Sakthivelu V, Jevtic M, Seeger JM, Müller S, et al.
Cell reports (2022) 387: 110370. 302 304 ICC; tested species: mouse

Centrosome-dependent microtubule modifications set the conditions for axon formation.
Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, Ruecker T, et al.
Cell reports (2022) 393: 110686. 302 304 ICC; tested species: rat

AP-4-mediated axonal transport controls endocannabinoid production in neurons.
Davies AK, Alecu JE, Ziegler M, Vasilopoulou CG, Merciai F, Jumo H, Afshar-Saber W, Sahin M, Ebrahimi-Fakhari D, Borner GHH
Nature communications (2022) 131: 1058. 302 304 ICC; tested species: mouse

Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro.
Pigareva Y, Gladkov A, Kolpakov V, Mukhina I, Bukatin A, Kazantsev VB, Pimashkin A
Brain sciences (2021) 116: . 302 304 ICC; tested species: mouse

Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.
Gunaseelan S, Wang Z, Tong VKJ, Ming SWS, Razar RBBA, Srimasorn S, Ong WY, Lim KL, Chua JJE
Human molecular genetics (2021) : . 302 304 ICC; tested species: human

Brain-Derived Neurotrophic Factor (BDNF) Preserves the Functional Integrity of Neural Networks in the β-Amyloidopathy Model in vitro.
Mitroshina EV, Yarkov RS, Mishchenko TA, Krut' VG, Gavrish MS, Epifanova EA, Babaev AA, Vedunova MV
Frontiers in cell and developmental biology (2020) 8: 582. 302 304 ICC; tested species: mouse

Design of Cultured Neuron Networks in vitro with Predefined Connectivity Using Asymmetric Microfluidic Channels.
Gladkov A, Pigareva Y, Kutyina D, Kolpakov V, Bukatin A, Mukhina I, Kazantsev V, Pimashkin A
Scientific reports (2017) 71: 15625. 302 304 ICC; tested species: mouse

Methyl-4-phenylpyridinium (MPP+) differentially affects monoamine release and re-uptake in murine embryonic stem cell-derived dopaminergic and serotonergic neurons.
Martí Y, Matthaeus F, Lau T, Schloss P
Molecular and cellular neurosciences (2017) 83: 37-45. 302 304 ICC; tested species: mouse

mGlu5 Receptors and Relapse to Cocaine-Seeking: The Role of Receptor Trafficking in Postrelapse Extinction Learning Deficits.
Knackstedt LA, Schwendt M
Neural plasticity (2016) 2016: 9312508. 302 304 ICC

Axonal transport and secretion of fibrillar forms of α-synuclein, Aβ42 peptide and HTTExon 1.
Brahic M, Bousset L, Bieri G, Melki R, Gitler AD
Acta neuropathologica (2016) 1314: 539-48. 302 304 ICC

Nongenomic, glucocorticoid receptor-mediated regulation of serotonin transporter cell surface expression in embryonic stem cell derived serotonergic neurons.
Lau T, Heimann F, Bartsch D, Schloss P, Weber T
Neuroscience letters (2013) 554: 115-20. 302 304 ICC; tested species: mouse

Morphological and functional differentiation in BE(2)-M17 human neuroblastoma cells by treatment with Trans-retinoic acid.
Andres D, Keyser BM, Petrali J, Benton B, Hubbard KS, McNutt PM, Ray R
BMC neuroscience (2013) 14: 49. 302 304 ICC

Radiology of inflammatory bowel disease.

Radiologic clinics of North America (1987) 251: 1-237. 302 304 ICC; tested species: human

The RNA-Binding Protein HuR Is Integral to the Function of Nociceptors in Mice and Humans.
Kunder N, de la Peña JB, Lou TF, Chase R, Suresh P, Lawson J, Shukla T, Black B, Campbell ZT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4249: 9129-9141. 302 304 IHC; tested species: mouse

Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 302 304 IHC; tested species: mouse

Egr2 overexpression in Schwann cells increases myelination frequency in vitro.
Tammia M, Mi R, Sluch VM, Zhu A, Chung T, Shinn D, Zack DJ, Höke A, Mao HQ
Heliyon (2018) 411: e00982. 302 304 IHC; tested species: rat

Cat. No.: 302 304

Amount: 100 µl

Price: $375.00

References
WB (1)
ICC (21)
IHC (3)

Radiology of inflammatory bowel disease.

Radiologic clinics of North America (1987) 251: 1-237. 302 304 ICC; tested species: human

ICC

IHC

IHC-P

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape (1).
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter (1).
Class III β-tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development (2).
It is widely used as a neuronal marker in normal and neoplastic tissues but has also been reported to be expressed in certain tumors of non-neuronal origin (3).

1 ) Katsetos CD et al., J. Child Neurol. (2003)
2 ) Katsetos CD et al., Cell Motil. Cytoskeleton (2003)
3 ) Dráberová E et al., Histochem. Cell Biol. (1998)
4 ) Koh Y et al., J Thorac Oncol (2010)
5 ) Jouhilahti EM et al., J. Histochem. Cytochem. (2008)
6 ) Sharma RK et al., Brain Res. (2007)
7 ) Stagi M et al., J. Neurosci. (2005)
8 ) Duittoz AH et al., J. Neurosci. Methods (2001)
9 ) Ferreira A et al., J. Neurosci. Res. (1992)
10 ) Lee MK et al., Proc. Natl. Acad. Sci. U.S.A. (1990)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Antigen retrieval
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

beta3-Tubulin antibody - 302 304

References for beta3-Tubulin - 302 304