|Cat. No. 302 304||100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery
ICC: 1 : 500 gallery
IHC: 1 : 200 (see remarks) gallery
IHC-P/FFPE: 1 : 200 up to 1 : 500 gallery
|Immunogen||Synthetic peptide corresponding to AA 443 to 450 from mouse β3-Tubulin (UniProt Id: Q9ERD7)|
Reacts with: human (Q13509), rat (Q4QRB4), mouse (Q9ERD7).
Other species not tested yet.
|Specificity||Specific for β3-tubulin.|
|Matching control protein/peptide||302-3P|
IHC: For best results we recommend antigen retrieval with pepsin according to Lorincz A & Nusser Z (2008).
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape (1).
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter (1).
Class III β-tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development (2).
It is widely used as a neuronal marker in normal and neoplastic tissues but has also been reported to be expressed in certain tumours of non-neuronal origin (3).