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beta3-Tubulin - 302 304

A tubulin isoform specific for neurons
Polyclonal Guinea pig antiserum
Cat. No.: 302 304
Amount: 100 µl
Price: $350.00
Cat. No. 302 304 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 (see remarks) gallery  
IHC-P/FFPE: 1 : 200 up to 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 443 to 450 from mouse β3-Tubulin (UniProt Id: Q9ERD7)
Reactivity Reacts with: human (Q13509), rat (Q4QRB4), mouse (Q9ERD7).
Other species not tested yet.
Specificity Specific for β3-tubulin.
Matching control protein/peptide 302-3P
Remarks

IHC: For best results we recommend antigen retrieval with pepsin according to Lorincz A & Nusser Z (2008).

Data sheet 302_304.pdf

References for beta3-Tubulin - 302 304

Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 302 304 WB; tested species: rat
Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.
Gunaseelan S, Wang Z, Tong VKJ, Ming SWS, Razar RBBA, Srimasorn S, Ong WY, Lim KL, Chua JJE
Human molecular genetics (2021) : . 302 304 ICC; tested species: human
Brain-Derived Neurotrophic Factor (BDNF) Preserves the Functional Integrity of Neural Networks in the β-Amyloidopathy Model in vitro.
Mitroshina EV, Yarkov RS, Mishchenko TA, Krut' VG, Gavrish MS, Epifanova EA, Babaev AA, Vedunova MV
Frontiers in cell and developmental biology (2020) 8: 582. 302 304 ICC; tested species: mouse
Methyl-4-phenylpyridinium (MPP+) differentially affects monoamine release and re-uptake in murine embryonic stem cell-derived dopaminergic and serotonergic neurons.
Martí Y, Matthaeus F, Lau T, Schloss P
Molecular and cellular neurosciences (2017) 83: 37-45. 302 304 ICC; tested species: mouse
Design of Cultured Neuron Networks in vitro with Predefined Connectivity Using Asymmetric Microfluidic Channels.
Gladkov A, Pigareva Y, Kutyina D, Kolpakov V, Bukatin A, Mukhina I, Kazantsev V, Pimashkin A
Scientific reports (2017) 71: 15625. 302 304 ICC; tested species: mouse
Axonal transport and secretion of fibrillar forms of α-synuclein, Aβ42 peptide and HTTExon 1.
Brahic M, Bousset L, Bieri G, Melki R, Gitler AD
Acta neuropathologica (2016) 1314: 539-48. 302 304 ICC
mGlu5 Receptors and Relapse to Cocaine-Seeking: The Role of Receptor Trafficking in Postrelapse Extinction Learning Deficits.
Knackstedt LA, Schwendt M
Neural plasticity (2016) 2016: 9312508. 302 304 ICC
Morphological and functional differentiation in BE(2)-M17 human neuroblastoma cells by treatment with Trans-retinoic acid.
Andres D, Keyser BM, Petrali J, Benton B, Hubbard KS, McNutt PM, Ray R
BMC neuroscience (2013) 14: 49. 302 304 ICC
Nongenomic, glucocorticoid receptor-mediated regulation of serotonin transporter cell surface expression in embryonic stem cell derived serotonergic neurons.
Lau T, Heimann F, Bartsch D, Schloss P, Weber T
Neuroscience letters (2013) 554: 115-20. 302 304 ICC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 302 304 IHC; tested species: mouse
Egr2 overexpression in Schwann cells increases myelination frequency in vitro.
Tammia M, Mi R, Sluch VM, Zhu A, Chung T, Shinn D, Zack DJ, Höke A, Mao HQ
Heliyon (2018) 411: e00982. 302 304 IHC; tested species: rat
Cat. No.: 302 304
Quantity: 100 µl
Price: $350.00
Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 302 304 WB; tested species: rat
Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.
Gunaseelan S, Wang Z, Tong VKJ, Ming SWS, Razar RBBA, Srimasorn S, Ong WY, Lim KL, Chua JJE
Human molecular genetics (2021) : . 302 304 ICC; tested species: human
Brain-Derived Neurotrophic Factor (BDNF) Preserves the Functional Integrity of Neural Networks in the β-Amyloidopathy Model in vitro.
Mitroshina EV, Yarkov RS, Mishchenko TA, Krut' VG, Gavrish MS, Epifanova EA, Babaev AA, Vedunova MV
Frontiers in cell and developmental biology (2020) 8: 582. 302 304 ICC; tested species: mouse
Methyl-4-phenylpyridinium (MPP+) differentially affects monoamine release and re-uptake in murine embryonic stem cell-derived dopaminergic and serotonergic neurons.
Martí Y, Matthaeus F, Lau T, Schloss P
Molecular and cellular neurosciences (2017) 83: 37-45. 302 304 ICC; tested species: mouse
Design of Cultured Neuron Networks in vitro with Predefined Connectivity Using Asymmetric Microfluidic Channels.
Gladkov A, Pigareva Y, Kutyina D, Kolpakov V, Bukatin A, Mukhina I, Kazantsev V, Pimashkin A
Scientific reports (2017) 71: 15625. 302 304 ICC; tested species: mouse
Axonal transport and secretion of fibrillar forms of α-synuclein, Aβ42 peptide and HTTExon 1.
Brahic M, Bousset L, Bieri G, Melki R, Gitler AD
Acta neuropathologica (2016) 1314: 539-48. 302 304 ICC
mGlu5 Receptors and Relapse to Cocaine-Seeking: The Role of Receptor Trafficking in Postrelapse Extinction Learning Deficits.
Knackstedt LA, Schwendt M
Neural plasticity (2016) 2016: 9312508. 302 304 ICC
Morphological and functional differentiation in BE(2)-M17 human neuroblastoma cells by treatment with Trans-retinoic acid.
Andres D, Keyser BM, Petrali J, Benton B, Hubbard KS, McNutt PM, Ray R
BMC neuroscience (2013) 14: 49. 302 304 ICC
Nongenomic, glucocorticoid receptor-mediated regulation of serotonin transporter cell surface expression in embryonic stem cell derived serotonergic neurons.
Lau T, Heimann F, Bartsch D, Schloss P, Weber T
Neuroscience letters (2013) 554: 115-20. 302 304 ICC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 302 304 IHC; tested species: mouse
Egr2 overexpression in Schwann cells increases myelination frequency in vitro.
Tammia M, Mi R, Sluch VM, Zhu A, Chung T, Shinn D, Zack DJ, Höke A, Mao HQ
Heliyon (2018) 411: e00982. 302 304 IHC; tested species: rat
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape (1).
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter (1).
Class III β-tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development (2).
It is widely used as a neuronal marker in normal and neoplastic tissues but has also been reported to be expressed in certain tumours of non-neuronal origin (3).