Synaptic Systems - Glu-Tubulin

Cat. No.: 302 011

Amount: 100 µg

Price: $420.00

Cat. No. 302 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 200 up to 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 1000 gallery ELISA: yes
Clone	1D5
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the c-terminus of human Glu-α-tubulin. (UniProt Id: Q71U36)
Epitop	AA 448 to 450 from human Glu-α-tubulin (UniProt Id: Q71U36)
Reactivity	Reacts with: human (Q71U36), rat (P68370), mouse (P68369), zebrafish, eukaryotes, other vertebrates. Other species not tested yet. Detects also cilia of Paramecium.
Specificity	Specific for detyrosinated α-tubulin (glu-tubulin) and polyglutamylated tubulin (also β-tubulin). No cross reaction to tyrosinated tubulin.
Remarks	WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal.
Data sheet	302_011.pdf

References for Glu-Tubulin - 302 011

Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells.
Zink S, Grosse L, Freikamp A, Bänfer S, Müksch F, Jacob R
Journal of cell science (2012) 125Pt 24: 5998-6008. 302 011 WB, ICC

A family of protein-deglutamylating enzymes associated with neurodegeneration.
Rogowski K, van Dijk J, Magiera MM, Bosc C, Deloulme JC, Bosson A, Peris L, Gold ND, Lacroix B, Bosch Grau M, Bec N, et al.
Cell (2010) 1434: 564-78. 302 011 WB

Turnover of the carboxy-terminal tyrosine of alpha-tubulin and means of reaching elevated levels of detyrosination in living cells.
Wehland J, Weber K
Journal of cell science (1987) 88 ( Pt 2): 185-203. 302 011 WB, ICC

Cat. No.: 302 011

Amount: 100 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric α-tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated α-tubulin is referred to as Glu-α-tubulin. Another post-translational modification of detyrosinated α-tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by tyrosine ligase and is one of the dominant α-tubulin isoforms in neurons.

1 ) Erck C et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
2 ) Contin MA et al., Biochem. J. (1999)
3 ) Paturle-Lafanechère L et al., J. Cell. Sci. (1994)
4 ) Ersfeld K et al., J. Cell Biol. (1993)
5 ) Rüdiger M et al., FEBS Lett. (1992)
6 ) Caron JM et al., J. Cell Biol. (1985)
7 ) Caron JM et al., Nature ()

Protocols

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Glu-Tubulin antibody - 302 011

References for Glu-Tubulin - 302 011