Synaptotagmin12 antibody - 299 003

Synaptotagmin12 is located to synaptic vesicles

Rabbit polyclonal purified antibody

Cat. No.: 299 003

Amount: 50 µg

Price: $455.00

Cat. No. 299 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 47 to 150 from rat Synaptotagmin12 (UniProt Id: P97610)
Reactivity	Reacts with: rat (P97610), mouse (Q920N7). Other species not tested yet.
Data sheet	299_003.pdf

References for Synaptotagmin12 - 299 003

The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 299 003 WB; tested species: mouse

Cat. No.: 299 003

Amount: 50 µg

Price: $455.00

Background

Up to now at least 17 synaptotagmins have been identified. Synaptotagmin12 is composed of a vesicular, a transmembrane and two C2 domains. It belongs to the calcium independent synaptotagmins and colocalizes with synaptotagmin1 on synaptic vesicles.

1 ) Kaeser-Woo YJ et al., J. Neurosci. (2013)
2 ) Maximov A et al., J. Cell Biol. (2007)

Protocols

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