Synaptotagmin12 antibody - 299 002

Synaptotagmin12 is located to synaptic vesicles

Rabbit polyclonal antiserum

Cat. No.: 299 002

Amount: 200 µl

Price: $360.00

Cat. No. 299 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 47 to 150 from rat Synaptotagmin12 (UniProt Id: P97610)
Reactivity	Reacts with: rat (P97610), mouse (Q920N7). Other species not tested yet.
Data sheet	Datasheet 299_002

Cat. No.: 299 002

Amount: 200 µl

Price: $360.00

Background

Up to now at least 17 synaptotagmins have been identified. Synaptotagmin12 is composed of a vesicular, a transmembrane and two C2 domains. It belongs to the calcium independent synaptotagmins and colocalizes with synaptotagmin1 on synaptic vesicles.

1 ) Kaeser-Woo YJ et al., J. Neurosci. (2013)
2 ) Maximov A et al., J. Cell Biol. (2007)

Protocols

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