ChAT antibody - 297 111

ChAT is an enzyme involved in the synthesis of acetylcholine

Mouse monoclonal purified IgG

Cat. No.: 297 111

Amount: 100 µg

Price: $415.00

Cat. No. 297 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 100 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	110G10
Subtype	IgG1
Immunogen	Recombinant protein corresponding to AA 2 to 640 from rat ChAT (UniProt Id: P32738)
Reactivity	Reacts with: mouse (Q03059), rat (P32738). Other species not tested yet.
Remarks	IHC: Compared to mouse tissue, this antibody shows a poorer signal to noise ratio on rat tissue. IHC-P: Compared to mouse tissue, this antibody shows a poorer signal to noise ratio on rat tissue.
Data sheet	297_111.pdf

Cat. No.: 297 111

Amount: 100 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Cholinergic neurons are involved in numerous neurological functions. Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons,postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells.
Two enzymes; choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) are involved in the metabolism of ACh.

1 ) Lorenzi MV et al., DNA Cell Biol. (1992)
2 ) Luine VN et al., Exp. Neurol. (1985)

Protocols

ICC

IHC

IHC-P

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