ChAT antibody - 297 003

ChAT is an enzyme involved in the synthesis of acetylcholine

Rabbit polyclonal purified antibody

Cat. No.: 297 003

Amount: 50 µg

Price: $375.00

Cat. No. 297 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 357 to 374 and 622 to 640 from rat ChAT (UniProt Id: P32738)
Reactivity	Reacts with: rat (P32738), mouse (Q03059). Other species not tested yet.
Data sheet	297_003.pdf

Cat. No.: 297 003

Amount: 50 µg

Price: $375.00

Background

Cholinergic neurons are involved in numerous neurological functions. Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons,postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells.
Two enzymes; choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) are involved in the metabolism of ACh.

1 ) Lorenzi MV et al., DNA Cell Biol. (1992)
2 ) Luine VN et al., Exp. Neurol. (1985)

Protocols

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