Olig2 antibody - 292 015

Olig2 is an oligodendrocyte specific transcription factor

Guinea pig polyclonal purified antibody

Cat. No.: 292 015

Amount: 50 µg

Price: $465.00

Cat. No. 292 015	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 2000 gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full length mouse recombinant Olig2 (UniProt Id: Q9EQW6)
Reactivity	Reacts with: mouse (Q9EQW6), rat (G3V612). Other species not tested yet. Predicted to cross-react with human (Q13516) due to high sequence homology.
Data sheet	292_015.pdf

Cat. No.: 292 015

Amount: 50 µg

Price: $465.00

IHC

IHC-P

Background

Olig 2, also referred to as BHLHB 1 and PRKCBP 2, is a transcription factor specific for the oligodendroglial lineage.
Oligodendrocytes are glia-cells that make and maintain the myelin of the central nervous system. The nuclei of these cells are in general smaller and have a more oval shape compared to nuclei from other cell types.

1 ) Mei F et al., J. Neurosci. (2013)
2 ) Chung SH et al., Cell Death Dis (2013)
3 ) Hafler BP et al., Proc. Natl. Acad. Sci. U.S.A. (2012)
4 ) Mikami S et al., Virchows Arch. (2007)
5 ) Yokoo H et al., Am. J. Pathol. (2004)
6 ) Marie Y et al., Lancet (2001)

Protocols

IHC

IHC-P

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