TNiK antibody - 290 003

TNiK is a kinase involved in the regulation of the cytoskeleton

Rabbit polyclonal purified antibody

Cat. No.: 290 003

Amount: 50 µg

Price: $380.00

Cat. No. 290 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 601 to 704 from mouse TNiK (UniProt Id: P83510)
Reactivity	Reacts with: rat, mouse (P83510). Other species not tested yet.
Data sheet	Datasheet 290_003

Cat. No.: 290 003

Amount: 50 µg

Price: $380.00

ICC

Background

Traf2- and Nck-interacting kinase (TNiK) is mainly expressed in muscle and brain. Upon overexpression it activates the c-Jun N-terminal kinase (JNK) pathway and leads to disruption of F-actin structure.
In neurons TNiK has been shown to be postsynaptically enriched in dendrites, where it is involved in Rap2 mediated signal transduction regulating neuronal structure and GluA receptor function.

1 ) Coba MP et al., J. Neurosci. (2012)
2 ) Hussain NK et al., J. Neurosci. (2010)
3 ) Fu CA et al., J. Biol. Chem. (1999)

Protocols

ICC

Fixed Cells