S100B antibody - 287 111

S100B is a marker for mature astrocytes

Mouse monoclonal purified IgG

Cat. No.: 287 111

Amount: 100 µg

Price: $415.00

Cat. No. 287 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 100 up to 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	86D7E4
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 92 from rat S100B (UniProt Id: P04631)
Reactivity	Reacts with: mouse (P50114), rat (P04631). Other species not tested yet.
Remarks	IHC-P: This antibody is highly recommended for IHC-P.
Data sheet	287_111.pdf

Cat. No.: 287 111

Amount: 100 µg

Price: $415.00

ICC

IHC

IHC-P

Background

The family of S100 proteins comprises more than 20 members. These proteins are EF-hand Ca²⁺-binding proteins, and are widely distributed in mammalian tissue. Since these proteins are soluble in 100 % saturated ammonium-sulfate solution they have been named S100.
S100B is a frequently used marker protein for mature astrocytes whereas GFAP is also expressed in germinal zone cells that maintained their immature developmental stage.

1 ) Gos T et al., J Psychiatr Res (2013)
2 ) Ondruschka B et al., J. Neurotrauma (2013)
3 ) Raponi E et al., Glia (2007)
4 ) Lin J et al., J. Biol. Chem. (2004)

Protocols

ICC

IHC

IHC-P

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