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Home > Products > 287 006

S100B antibody - 287 006

S100B is a marker for mature astrocytes
Chicken polyclonal purified antibody
Cat. No.: 287 006
Amount: 50 µg
Price: $385.00
Cat. No. 287 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 up to 1 : 1000      gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 92 from rat S100B (UniProt Id: P04631)
Reactivity Reacts with: rat (P04631), mouse (P50114), human (P04271).
Other species not tested yet.
Data sheet 287_006.pdf

References for S100B - 287 006

Astrocytic autophagy plasticity modulates Aβ clearance and cognitive function in Alzheimer's disease.
Kim S, Chun H, Kim Y, Kim Y, Park U, Chu J, Bhalla M, Choi SH, Yousefian-Jazi A, Kim S, Hyeon SJ, et al.
Molecular neurodegeneration (2024) 191: 55. 287 006 ICC; tested species: mouse
Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing.
Tetzlaff SK, Reyhan E, Layer N, Bengtson CP, Heuer A, Schroers J, Faymonville AJ, Langeroudi AP, Drewa N, Keifert E, Wagner J, et al.
Cell (2024) : . 287 006 ICC, IHC-P; tested species: human,mouse
LSD1 Regulates Neurogenesis in Human Neural Stem Cells Through the Repression of Human-Enriched Extracellular Matrix and Cell Adhesion Genes.
Channakkar AS, D'Souza L, Kumar A, Kalia K, Prabhu S, Phalnikar K, Reddy PC, Muralidharan B
Stem cells (Dayton, Ohio) (2023) : . 287 006 ICC; tested species: human
Obesity-Induced Cellular Senescence Drives Anxiety and Impairs Neurogenesis.
Ogrodnik M, Zhu Y, Langhi LGP, Tchkonia T, Krüger P, Fielder E, Victorelli S, Ruswhandi RA, Giorgadze N, Pirtskhalava T, Podgorni O, et al.
Cell metabolism (2018) : . 287 006 ICC; tested species: mouse
Ultrasonocoverslip: In-vitro platform for high-throughput assay of cell type-specific neuromodulation with ultra-low-intensity ultrasound stimulation.
Lee K, Lee JM, Phan TT, Lee CJ, Park JM, Park J
Brain stimulation (2023) 165: 1533-1548. 287 006 IHC; tested species: mouse
Neuronal and astrocytic contributions to Huntington's disease dissected with zinc finger protein transcriptional repressors.
Gangwani MR, Soto JS, Jami-Alahmadi Y, Tiwari S, Kawaguchi R, Wohlschlegel JA, Khakh BS
Cell reports (2023) 421: 111953. 287 006 IHC; tested species: mouse
Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.
Noguchi H, Arela JC, Ngo TT, Cocas L, Pleasure SJ
bioRxiv : the preprint server for biology (2023) : . 287 006 IHC; tested species: mouse
Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.
Noguchi H, Arela JC, Ngo T, Cocas L, Pleasure S
eLife (2023) 12: . 287 006 IHC; tested species: mouse
Sexually Dimorphic Effects of Histamine Degradation by Enteric Glial Histamine N-Methyltransferase (HNMT) on Visceral Hypersensitivity.
McClain JL, Morales-Soto W, Gonzales J, Parmar V, Demireva EY, Gulbransen BD
Biomolecules (2023) 1311: . 287 006 IHC; tested species: mouse
Glioblastoma hijacks neuronal mechanisms for brain invasion.
Venkataramani V, Yang Y, Schubert MC, Reyhan E, Tetzlaff SK, Wißmann N, Botz M, Soyka SJ, Beretta CA, Pramatarov RL, Fankhauser L, et al.
Cell (2022) : . 287 006 IHC; tested species: mouse
Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain.
Venturino A, Schulz R, De Jesús-Cortés H, Maes ME, Nagy B, Reilly-Andújar F, Colombo G, Cubero RJA, Schoot Uiterkamp FE, Bear MF, Siegert S, et al.
Cell reports (2021) 361: 109313. 287 006 IHC; tested species: mouse
Autoantibodies against NMDA receptor 1 modify rather than cause encephalitis.
Wilke JBH, Hindermann M, Berghoff SA, Zihsler S, Arinrad S, Ronnenberg A, Barnkothe N, Steixner-Kumar AA, Röglin S, Stöcker W, Hollmann M, et al.
Molecular psychiatry (2021) : . 287 006 IHC; tested species: mouse
Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction.
Konishi H, Okamoto T, Hara Y, Komine O, Tamada H, Maeda M, Osako F, Kobayashi M, Nishiyama A, Kataoka Y, Takai T, et al.
The EMBO journal (2020) 3922: e104464. 287 006 IHC; tested species: mouse
SRF Is Required for Maintenance of Astrocytes in Non-Reactive State in the Mammalian Brain.
Jain M, Das S, Lu PPY, Virmani G, Soman S, Thumu SCR, Gutmann DH, Ramanan N
eNeuro () 81: . 287 006 IHC; tested species: mouse
Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing.
Tetzlaff SK, Reyhan E, Layer N, Bengtson CP, Heuer A, Schroers J, Faymonville AJ, Langeroudi AP, Drewa N, Keifert E, Wagner J, et al.
Cell (2024) : . 287 006 ICC, IHC-P; tested species: human,mouse
Astrocytes expressing Vitamin D-activating enzyme identify Parkinson's disease.
Mazzetti S, Barichella M, Giampietro F, Giana A, Calogero AM, Amadeo A, Palazzi N, Comincini A, Giaccone G, Bramerio M, Caronni S, et al.
CNS neuroscience & therapeutics (2022) : . 287 006 IHC-P; tested species: human
Cat. No.: 287 006
Amount: 50 µg
Price: $385.00
Astrocytic autophagy plasticity modulates Aβ clearance and cognitive function in Alzheimer's disease.
Kim S, Chun H, Kim Y, Kim Y, Park U, Chu J, Bhalla M, Choi SH, Yousefian-Jazi A, Kim S, Hyeon SJ, et al.
Molecular neurodegeneration (2024) 191: 55. 287 006 ICC; tested species: mouse
Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing.
Tetzlaff SK, Reyhan E, Layer N, Bengtson CP, Heuer A, Schroers J, Faymonville AJ, Langeroudi AP, Drewa N, Keifert E, Wagner J, et al.
Cell (2024) : . 287 006 ICC, IHC-P; tested species: human,mouse
LSD1 Regulates Neurogenesis in Human Neural Stem Cells Through the Repression of Human-Enriched Extracellular Matrix and Cell Adhesion Genes.
Channakkar AS, D'Souza L, Kumar A, Kalia K, Prabhu S, Phalnikar K, Reddy PC, Muralidharan B
Stem cells (Dayton, Ohio) (2023) : . 287 006 ICC; tested species: human
Obesity-Induced Cellular Senescence Drives Anxiety and Impairs Neurogenesis.
Ogrodnik M, Zhu Y, Langhi LGP, Tchkonia T, Krüger P, Fielder E, Victorelli S, Ruswhandi RA, Giorgadze N, Pirtskhalava T, Podgorni O, et al.
Cell metabolism (2018) : . 287 006 ICC; tested species: mouse
Ultrasonocoverslip: In-vitro platform for high-throughput assay of cell type-specific neuromodulation with ultra-low-intensity ultrasound stimulation.
Lee K, Lee JM, Phan TT, Lee CJ, Park JM, Park J
Brain stimulation (2023) 165: 1533-1548. 287 006 IHC; tested species: mouse
Neuronal and astrocytic contributions to Huntington's disease dissected with zinc finger protein transcriptional repressors.
Gangwani MR, Soto JS, Jami-Alahmadi Y, Tiwari S, Kawaguchi R, Wohlschlegel JA, Khakh BS
Cell reports (2023) 421: 111953. 287 006 IHC; tested species: mouse
Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.
Noguchi H, Arela JC, Ngo TT, Cocas L, Pleasure SJ
bioRxiv : the preprint server for biology (2023) : . 287 006 IHC; tested species: mouse
Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.
Noguchi H, Arela JC, Ngo T, Cocas L, Pleasure S
eLife (2023) 12: . 287 006 IHC; tested species: mouse
Sexually Dimorphic Effects of Histamine Degradation by Enteric Glial Histamine N-Methyltransferase (HNMT) on Visceral Hypersensitivity.
McClain JL, Morales-Soto W, Gonzales J, Parmar V, Demireva EY, Gulbransen BD
Biomolecules (2023) 1311: . 287 006 IHC; tested species: mouse
Glioblastoma hijacks neuronal mechanisms for brain invasion.
Venkataramani V, Yang Y, Schubert MC, Reyhan E, Tetzlaff SK, Wißmann N, Botz M, Soyka SJ, Beretta CA, Pramatarov RL, Fankhauser L, et al.
Cell (2022) : . 287 006 IHC; tested species: mouse
Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain.
Venturino A, Schulz R, De Jesús-Cortés H, Maes ME, Nagy B, Reilly-Andújar F, Colombo G, Cubero RJA, Schoot Uiterkamp FE, Bear MF, Siegert S, et al.
Cell reports (2021) 361: 109313. 287 006 IHC; tested species: mouse
Autoantibodies against NMDA receptor 1 modify rather than cause encephalitis.
Wilke JBH, Hindermann M, Berghoff SA, Zihsler S, Arinrad S, Ronnenberg A, Barnkothe N, Steixner-Kumar AA, Röglin S, Stöcker W, Hollmann M, et al.
Molecular psychiatry (2021) : . 287 006 IHC; tested species: mouse
Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction.
Konishi H, Okamoto T, Hara Y, Komine O, Tamada H, Maeda M, Osako F, Kobayashi M, Nishiyama A, Kataoka Y, Takai T, et al.
The EMBO journal (2020) 3922: e104464. 287 006 IHC; tested species: mouse
SRF Is Required for Maintenance of Astrocytes in Non-Reactive State in the Mammalian Brain.
Jain M, Das S, Lu PPY, Virmani G, Soman S, Thumu SCR, Gutmann DH, Ramanan N
eNeuro () 81: . 287 006 IHC; tested species: mouse
Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing.
Tetzlaff SK, Reyhan E, Layer N, Bengtson CP, Heuer A, Schroers J, Faymonville AJ, Langeroudi AP, Drewa N, Keifert E, Wagner J, et al.
Cell (2024) : . 287 006 ICC, IHC-P; tested species: human,mouse
Astrocytes expressing Vitamin D-activating enzyme identify Parkinson's disease.
Mazzetti S, Barichella M, Giampietro F, Giana A, Calogero AM, Amadeo A, Palazzi N, Comincini A, Giaccone G, Bramerio M, Caronni S, et al.
CNS neuroscience & therapeutics (2022) : . 287 006 IHC-P; tested species: human
ICC
IHC
IHC-P
IHC-P
Background

The family of S100 proteins comprises more than 20 members. These proteins are EF-hand Ca2+-binding proteins, and are widely distributed in mammalian tissue. Since these proteins are soluble in 100 % saturated ammonium-sulfate solution they have been named S100.
S100B is a frequently used marker protein for mature astrocytes whereas GFAP is also expressed in germinal zone cells that maintained their immature developmental stage.
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