Synaptic Systems

Cat. No.: 287 004

Amount: 100 µl

Price: $400.00

Cat. No. 287 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 gallery IHC-G: 1 : 500 (see remarks) gallery Clarity: 1 : 200 (see remarks)
Immunogen	Recombinant protein corresponding to AA 1 to 92 from rat S100B (UniProt Id: P04631)
Reactivity	Reacts with: rat (P04631), mouse (P50114), human (P04271). Other species not tested yet.
Remarks	IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017, or 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 are possible. Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).
Data sheet	287_004.pdf

References for S100B - 287 004

WB (2)
ICC (3)
IHC (24)
Clarity (1)

S100B dysregulation during brain development affects synaptic SHANK protein networks via alteration of zinc homeostasis.
Daini E, Hagmeyer S, De Benedictis CA, Cristóvão JS, Bodria M, Ross AM, Raab A, Boeckers TM, Feldmann J, Gomes CM, Zoli M, et al.
Translational psychiatry (2021) 111: 562. 287 004 WB; tested species: rat

Parvalbumin-expressing ependymal cells in rostral lateral ventricle wall adhesions contribute to aging-related ventricle stenosis in mice.
Filice F, Celio MR, Babalian A, Blum W, Szabolcsi V
The Journal of comparative neurology (2017) 52515: 3266-3285. 287 004 IHC, WB; tested species: mouse

Transient ocular hypertension remodels astrocytes through S100B.
Huang W, Matsushita K, Kawashima R, Hara S, Yasukura Y, Yamaguchi K, Usui S, Baba K, Quantock AJ, Nishida K
PloS one (2025) 202: e0313556. 287 004 ICC; tested species: mouse

An implantable human stem cell-derived tissue-engineered rostral migratory stream for directed neuronal replacement.
O'Donnell JC, Purvis EM, Helm KVT, Adewole DO, Zhang Q, Le AD, Cullen DK
Communications biology (2021) 41: 879. 287 004 ICC; tested species: human

Zinc Binding to S100B Affords Regulation of Trace Metal Homeostasis and Excitotoxicity in the Brain.
Hagmeyer S, Cristóvão JS, Mulvihill JJE, Boeckers TM, Gomes CM, Grabrucker AM
Frontiers in molecular neuroscience (2017) 10: 456. 287 004 ICC; tested species: rat

EZH2-dependent myelination following sciatic nerve injury.
Zhu H, Mu L, Xu X, Huang T, Wang Y, Xu S, Wang Y, Wang W, Wang Z, Wang H, Xue C, et al.
Neural regeneration research (2025) 208: 2382-2394. 287 004 IHC; tested species: mouse

Neonatal brain injury unravels transcriptional and signaling changes underlying the reactivation of cortical progenitors.
Foucault L, Capeliez T, Angonin D, Lentini C, Bezin L, Heinrich C, Parras C, Donega V, Marcy G, Raineteau O
Cell reports (2024) 432: 113734. 287 004 IHC; tested species: mouse

Rapid modulation of striatal cholinergic interneurons and dopamine release by satellite astrocytes.
Stedehouder J, Roberts BM, Raina S, Bossi S, Liu AKL, Doig NM, McGerty K, Magill PJ, Parkkinen L, Cragg SJ
Nature communications (2024) 151: 10017. 287 004 IHC; tested species: mouse

Modeling blood-brain barrier formation and cerebral cavernous malformations in human PSC-derived organoids.
Dao L, You Z, Lu L, Xu T, Sarkar AK, Zhu H, Liu M, Calandrelli R, Yoshida G, Lin P, Miao Y, et al.
Cell stem cell (2024) : . 287 004 IHC; tested species: human

Rescue of astrocyte activity by the calcium sensor STIM1 restores long-term synaptic plasticity in female mice modelling Alzheimer's disease.
Lia A, Sansevero G, Chiavegato A, Sbrissa M, Pendin D, Mariotti L, Pozzan T, Berardi N, Carmignoto G, Fasolato C, Zonta M, et al.
Nature communications (2023) 141: 1590. 287 004 IHC; tested species: mouse

Stress induces behavioral abnormalities by increasing expression of phagocytic receptor, MERTK, in astrocytes to promote synapse phagocytosis.
Byun YG, Kim NS, Kim G, Jeon YS, Choi JB, Park CW, Kim K, Jang H, Kim J, Kim E, Han YM, et al.
Immunity (2023) : . 287 004 IHC; tested species: mouse

Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex.
Henriques VJ, Chiavegato A, Carmignoto G, Gómez-Gonzalo M
Cells (2022) 119: . 287 004 IHC; tested species: mouse

A central role for anterior cingulate cortex in the control of pathological aggression.
van Heukelum S, Tulva K, Geers FE, van Dulm S, Ruisch IH, Mill J, Viana JF, Beckmann CF, Buitelaar JK, Poelmans G, Glennon JC, et al.
Current biology : CB (2021) 3111: 2321-2333.e5. 287 004 IHC; tested species: mouse

Gamma-Aminobutyric Acid Transporters in the Nucleus Tractus Solitarii Regulate Inhibitory and Excitatory Synaptic Currents That Influence Cardiorespiratory Function.
Martinez D, Lima-Silveira L, Matott MP, Hasser EM, Kline DD
Frontiers in physiology (2021) 12: 821110. 287 004 IHC; tested species: rat

GSK-3β S9A overexpression leads murine hippocampal neural precursors to acquire an astroglial phenotype in vivo.
Flor-García M, Ávila J, Llorens-Martín M
Developmental neurobiology (2021) 815: 710-723. 287 004 IHC; tested species: mouse

Influence of Tilia tomentosa Moench Extract on Mouse Small Intestine Neuromuscular Contractility.
Cerantola S, Faggin S, Annaloro G, Mainente F, Filippini R, Savarino EV, Piovan A, Zoccatelli G, Giron MC
Nutrients (2021) 1310: . 287 004 IHC; tested species: mouse

Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice.
Schob S, Puchta J, Winter K, Michalski D, Mages B, Martens H, Emmer A, Hoffmann KT, Gaunitz F, Meinicke A, Krause M, et al.
Journal of chemical neuroanatomy (2021) 118: 102036. 287 004 IHC; tested species: mouse

P75 neurotrophin receptor controls subventricular zone neural stem cell migration after stroke.
Deshpande SS, Malik SC, Conforti P, Lin JD, Chu YH, Nath S, Greulich F, Dumbach MA, Uhlenhaut NH, Schachtrup C
Cell and tissue research (2021) : . 287 004 IHC; tested species: mouse

Astrocyte-derived neurons provide excitatory input to the adult striatal circuitry.
Dorst MC, Díaz-Moreno M, Dias DO, Guimarães EL, Holl D, Kalkitsas J, Silberberg G, Göritz C
Proceedings of the National Academy of Sciences of the United States of America (2021) 11833: . 287 004 IHC; tested species: mouse

Structural Degradation in Midcingulate Cortex Is Associated with Pathological Aggression in Mice.
Heukelum SV, Geers FE, Tulva K, van Dulm S, Beckmann CF, Buitelaar JK, Glennon JC, Vogt BA, Havenith MN, França ASC
Brain sciences (2021) 117: . 287 004 IHC; tested species: mouse

Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis.
Lee JH, Kim JY, Noh S, Lee H, Lee SY, Mun JY, Park H, Chung WS
Nature (2021) 5907847: 612-617. 287 004 IHC; tested species: mouse

Hippocampal neurons respond to brain activity with functional hypoxia.
Butt UJ, Steixner-Kumar AA, Depp C, Sun T, Hassouna I, Wüstefeld L, Arinrad S, Zillmann MR, Schopf N, Fernandez Garcia-Agudo L, Mohrmann L, et al.
Molecular psychiatry (2021) : . 287 004 IHC; tested species: mouse

S100β-mediated astroglial control of firing and input processing in layer 5 pyramidal neurons of the mouse visual cortex.
Ryczko D, Hanini-Daoud M, Condamine S, Bréant BJB, Fougère M, Araya R, Kolta A
The Journal of physiology (2020) : . 287 004 IHC; tested species: mouse

GABA uptake transporters support dopamine release in dorsal striatum with maladaptive downregulation in a parkinsonism model.
Roberts BM, Doig NM, Brimblecombe KR, Lopes EF, Siddorn RE, Threlfell S, Connor-Robson N, Bengoa-Vergniory N, Pasternack N, Wade-Martins R, Magill PJ, et al.
Nature communications (2020) 111: 4958. 287 004 IHC; tested species: mouse

Astroglial dysfunctions drive aberrant synaptogenesis and social behavioral deficits in mice with neonatal exposure to lengthy general anesthesia.
Zhou B, Chen L, Liao P, Huang L, Chen Z, Liao D, Yang L, Wang J, Yu G, Wang L, Zhang J, et al.
PLoS biology (2019) 178: e3000086. 287 004 IHC; tested species: mouse

AGO CLIP Reveals an Activated Network for Acute Regulation of Brain Glutamate Homeostasis in Ischemic Stroke.
Kobayashi M, Benakis C, Anderson C, Moore MJ, Poon C, Uekawa K, Dyke JP, Fak JJ, Mele A, Park CY, Zhou P, et al.
Cell reports (2019) 284: 979-991.e6. 287 004 IHC; tested species: mouse

Radial glial elements in the cerebral cortex of the lesser hedgehog tenrec.
Mack AF, Künzle H, Lange M, Mages B, Reichenbach A, Härtig W
Brain structure & function (2018) : . 287 004 IHC

Glucocorticoid receptor signaling in astrocytes is required for aversive memory formation.
Tertil M, Skupio U, Barut J, Dubovyk V, Wawrzczak-Bargiela A, Soltys Z, Golda S, Kudla L, Wiktorowska L, Szklarczyk K, Korostynski M, et al.
Translational psychiatry (2018) 81: 255. 287 004 IHC; tested species: mouse

CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.
Woelfle S, Deshpande D, Feldengut S, Braak H, Del Tredici K, Roselli F, Deisseroth K, Michaelis J, Boeckers TM, Schön M
BMC biology (2023) 211: 113. 287 004 Clarity; tested species: human

Cat. No.: 287 004

Amount: 100 µl

Price: $400.00

References
WB (2)
ICC (3)
IHC (24)
Clarity (1)

Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex.
Henriques VJ, Chiavegato A, Carmignoto G, Gómez-Gonzalo M
Cells (2022) 119: . 287 004 IHC; tested species: mouse

Radial glial elements in the cerebral cortex of the lesser hedgehog tenrec.
Mack AF, Künzle H, Lange M, Mages B, Reichenbach A, Härtig W
Brain structure & function (2018) : . 287 004 IHC

ICC

IHC

IHC-P

IHC-G

Background

The family of S100 proteins comprises more than 20 members. These proteins are EF-hand Ca²⁺-binding proteins, and are widely distributed in mammalian tissue. Since these proteins are soluble in 100 % saturated ammonium-sulfate solution they have been named S100.
S100B is a frequently used marker protein for mature astrocytes whereas GFAP is also expressed in germinal zone cells that maintained their immature developmental stage.

1 ) Gos T et al., J Psychiatr Res (2013)
2 ) Ondruschka B et al., J. Neurotrauma (2013)
3 ) Raponi E et al., Glia (2007)
4 ) Lin J et al., J. Biol. Chem. (2004)

Protocols

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

IHC-G

Free floating
Slide mounted
Tissue preparation

Other products for this target

S100B antibody - 287 004

References for S100B - 287 004