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Home > Products > 287 004

S100B antibody - 287 004

S100B is a marker for mature astrocytes
Guinea pig polyclonal antiserum
Cat. No.: 287 004
Amount: 100 µl
Price: $395.00
Cat. No. 287 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 200 gallery  
IHC-G: 1 : 500 (see remarks) gallery  
Clarity: 1 : 200 (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.

Clarity is a sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. Clearing is carried out by SDS and Focusclear treatment. For additional experimental details, please refer to the Remarks section.
Immunogen Recombinant protein corresponding to AA 1 to 92 from rat S100B (UniProt Id: P04631)
Reactivity Reacts with: rat (P04631), mouse (P50114), human (P04271).
Other species not tested yet.
Remarks

IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017, or 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 are possible.
Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).
Data sheet 287_004.pdf

References for S100B - 287 004

S100B dysregulation during brain development affects synaptic SHANK protein networks via alteration of zinc homeostasis.
Daini E, Hagmeyer S, De Benedictis CA, Cristóvão JS, Bodria M, Ross AM, Raab A, Boeckers TM, Feldmann J, Gomes CM, Zoli M, et al.
Translational psychiatry (2021) 111: 562. 287 004 WB; tested species: rat
Parvalbumin-expressing ependymal cells in rostral lateral ventricle wall adhesions contribute to aging-related ventricle stenosis in mice.
Filice F, Celio MR, Babalian A, Blum W, Szabolcsi V
The Journal of comparative neurology (2017) 52515: 3266-3285. 287 004 IHC, WB; tested species: mouse
An implantable human stem cell-derived tissue-engineered rostral migratory stream for directed neuronal replacement.
O'Donnell JC, Purvis EM, Helm KVT, Adewole DO, Zhang Q, Le AD, Cullen DK
Communications biology (2021) 41: 879. 287 004 ICC; tested species: human
Zinc Binding to S100B Affords Regulation of Trace Metal Homeostasis and Excitotoxicity in the Brain.
Hagmeyer S, Cristóvão JS, Mulvihill JJE, Boeckers TM, Gomes CM, Grabrucker AM
Frontiers in molecular neuroscience (2017) 10: 456. 287 004 ICC; tested species: rat
Modeling blood-brain barrier formation and cerebral cavernous malformations in human PSC-derived organoids.
Dao L, You Z, Lu L, Xu T, Sarkar AK, Zhu H, Liu M, Calandrelli R, Yoshida G, Lin P, Miao Y, et al.
Cell stem cell (2024) : . 287 004 IHC; tested species: human
Rescue of astrocyte activity by the calcium sensor STIM1 restores long-term synaptic plasticity in female mice modelling Alzheimer's disease.
Lia A, Sansevero G, Chiavegato A, Sbrissa M, Pendin D, Mariotti L, Pozzan T, Berardi N, Carmignoto G, Fasolato C, Zonta M, et al.
Nature communications (2023) 141: 1590. 287 004 IHC; tested species: mouse
Stress induces behavioral abnormalities by increasing expression of phagocytic receptor, MERTK, in astrocytes to promote synapse phagocytosis.
Byun YG, Kim NS, Kim G, Jeon YS, Choi JB, Park CW, Kim K, Jang H, Kim J, Kim E, Han YM, et al.
Immunity (2023) : . 287 004 IHC; tested species: mouse
Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex.
Henriques VJ, Chiavegato A, Carmignoto G, Gómez-Gonzalo M
Cells (2022) 119: . 287 004 IHC; tested species: mouse
P75 neurotrophin receptor controls subventricular zone neural stem cell migration after stroke.
Deshpande SS, Malik SC, Conforti P, Lin JD, Chu YH, Nath S, Greulich F, Dumbach MA, Uhlenhaut NH, Schachtrup C
Cell and tissue research (2021) : . 287 004 IHC; tested species: mouse
Gamma-Aminobutyric Acid Transporters in the Nucleus Tractus Solitarii Regulate Inhibitory and Excitatory Synaptic Currents That Influence Cardiorespiratory Function.
Martinez D, Lima-Silveira L, Matott MP, Hasser EM, Kline DD
Frontiers in physiology (2021) 12: 821110. 287 004 IHC; tested species: rat
GSK-3β S9A overexpression leads murine hippocampal neural precursors to acquire an astroglial phenotype in vivo.
Flor-García M, Ávila J, Llorens-Martín M
Developmental neurobiology (2021) 815: 710-723. 287 004 IHC; tested species: mouse
Influence of Tilia tomentosa Moench Extract on Mouse Small Intestine Neuromuscular Contractility.
Cerantola S, Faggin S, Annaloro G, Mainente F, Filippini R, Savarino EV, Piovan A, Zoccatelli G, Giron MC
Nutrients (2021) 1310: . 287 004 IHC; tested species: mouse
A central role for anterior cingulate cortex in the control of pathological aggression.
van Heukelum S, Tulva K, Geers FE, van Dulm S, Ruisch IH, Mill J, Viana JF, Beckmann CF, Buitelaar JK, Poelmans G, Glennon JC, et al.
Current biology : CB (2021) 3111: 2321-2333.e5. 287 004 IHC; tested species: mouse
Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice.
Schob S, Puchta J, Winter K, Michalski D, Mages B, Martens H, Emmer A, Hoffmann KT, Gaunitz F, Meinicke A, Krause M, et al.
Journal of chemical neuroanatomy (2021) 118: 102036. 287 004 IHC; tested species: mouse
Astrocyte-derived neurons provide excitatory input to the adult striatal circuitry.
Dorst MC, Díaz-Moreno M, Dias DO, Guimarães EL, Holl D, Kalkitsas J, Silberberg G, Göritz C
Proceedings of the National Academy of Sciences of the United States of America (2021) 11833: . 287 004 IHC; tested species: mouse
Structural Degradation in Midcingulate Cortex Is Associated with Pathological Aggression in Mice.
Heukelum SV, Geers FE, Tulva K, van Dulm S, Beckmann CF, Buitelaar JK, Glennon JC, Vogt BA, Havenith MN, França ASC
Brain sciences (2021) 117: . 287 004 IHC; tested species: mouse
Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis.
Lee JH, Kim JY, Noh S, Lee H, Lee SY, Mun JY, Park H, Chung WS
Nature (2021) 5907847: 612-617. 287 004 IHC; tested species: mouse
Hippocampal neurons respond to brain activity with functional hypoxia.
Butt UJ, Steixner-Kumar AA, Depp C, Sun T, Hassouna I, Wüstefeld L, Arinrad S, Zillmann MR, Schopf N, Fernandez Garcia-Agudo L, Mohrmann L, et al.
Molecular psychiatry (2021) : . 287 004 IHC; tested species: mouse
S100β-mediated astroglial control of firing and input processing in layer 5 pyramidal neurons of the mouse visual cortex.
Ryczko D, Hanini-Daoud M, Condamine S, Bréant BJB, Fougère M, Araya R, Kolta A
The Journal of physiology (2020) : . 287 004 IHC; tested species: mouse
GABA uptake transporters support dopamine release in dorsal striatum with maladaptive downregulation in a parkinsonism model.
Roberts BM, Doig NM, Brimblecombe KR, Lopes EF, Siddorn RE, Threlfell S, Connor-Robson N, Bengoa-Vergniory N, Pasternack N, Wade-Martins R, Magill PJ, et al.
Nature communications (2020) 111: 4958. 287 004 IHC; tested species: mouse
Astroglial dysfunctions drive aberrant synaptogenesis and social behavioral deficits in mice with neonatal exposure to lengthy general anesthesia.
Zhou B, Chen L, Liao P, Huang L, Chen Z, Liao D, Yang L, Wang J, Yu G, Wang L, Zhang J, et al.
PLoS biology (2019) 178: e3000086. 287 004 IHC; tested species: mouse
AGO CLIP Reveals an Activated Network for Acute Regulation of Brain Glutamate Homeostasis in Ischemic Stroke.
Kobayashi M, Benakis C, Anderson C, Moore MJ, Poon C, Uekawa K, Dyke JP, Fak JJ, Mele A, Park CY, Zhou P, et al.
Cell reports (2019) 284: 979-991.e6. 287 004 IHC; tested species: mouse
Radial glial elements in the cerebral cortex of the lesser hedgehog tenrec.
Mack AF, Künzle H, Lange M, Mages B, Reichenbach A, Härtig W
Brain structure & function (2018) : . 287 004 IHC
Glucocorticoid receptor signaling in astrocytes is required for aversive memory formation.
Tertil M, Skupio U, Barut J, Dubovyk V, Wawrzczak-Bargiela A, Soltys Z, Golda S, Kudla L, Wiktorowska L, Szklarczyk K, Korostynski M, et al.
Translational psychiatry (2018) 81: 255. 287 004 IHC; tested species: mouse
Parvalbumin-expressing ependymal cells in rostral lateral ventricle wall adhesions contribute to aging-related ventricle stenosis in mice.
Filice F, Celio MR, Babalian A, Blum W, Szabolcsi V
The Journal of comparative neurology (2017) 52515: 3266-3285. 287 004 IHC, WB; tested species: mouse
CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.
Woelfle S, Deshpande D, Feldengut S, Braak H, Del Tredici K, Roselli F, Deisseroth K, Michaelis J, Boeckers TM, Schön M
BMC biology (2023) 211: 113. 287 004 Clarity; tested species: human
Cat. No.: 287 004
Amount: 100 µl
Price: $395.00
S100B dysregulation during brain development affects synaptic SHANK protein networks via alteration of zinc homeostasis.
Daini E, Hagmeyer S, De Benedictis CA, Cristóvão JS, Bodria M, Ross AM, Raab A, Boeckers TM, Feldmann J, Gomes CM, Zoli M, et al.
Translational psychiatry (2021) 111: 562. 287 004 WB; tested species: rat
Parvalbumin-expressing ependymal cells in rostral lateral ventricle wall adhesions contribute to aging-related ventricle stenosis in mice.
Filice F, Celio MR, Babalian A, Blum W, Szabolcsi V
The Journal of comparative neurology (2017) 52515: 3266-3285. 287 004 IHC, WB; tested species: mouse
An implantable human stem cell-derived tissue-engineered rostral migratory stream for directed neuronal replacement.
O'Donnell JC, Purvis EM, Helm KVT, Adewole DO, Zhang Q, Le AD, Cullen DK
Communications biology (2021) 41: 879. 287 004 ICC; tested species: human
Zinc Binding to S100B Affords Regulation of Trace Metal Homeostasis and Excitotoxicity in the Brain.
Hagmeyer S, Cristóvão JS, Mulvihill JJE, Boeckers TM, Gomes CM, Grabrucker AM
Frontiers in molecular neuroscience (2017) 10: 456. 287 004 ICC; tested species: rat
Modeling blood-brain barrier formation and cerebral cavernous malformations in human PSC-derived organoids.
Dao L, You Z, Lu L, Xu T, Sarkar AK, Zhu H, Liu M, Calandrelli R, Yoshida G, Lin P, Miao Y, et al.
Cell stem cell (2024) : . 287 004 IHC; tested species: human
Rescue of astrocyte activity by the calcium sensor STIM1 restores long-term synaptic plasticity in female mice modelling Alzheimer's disease.
Lia A, Sansevero G, Chiavegato A, Sbrissa M, Pendin D, Mariotti L, Pozzan T, Berardi N, Carmignoto G, Fasolato C, Zonta M, et al.
Nature communications (2023) 141: 1590. 287 004 IHC; tested species: mouse
Stress induces behavioral abnormalities by increasing expression of phagocytic receptor, MERTK, in astrocytes to promote synapse phagocytosis.
Byun YG, Kim NS, Kim G, Jeon YS, Choi JB, Park CW, Kim K, Jang H, Kim J, Kim E, Han YM, et al.
Immunity (2023) : . 287 004 IHC; tested species: mouse
Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex.
Henriques VJ, Chiavegato A, Carmignoto G, Gómez-Gonzalo M
Cells (2022) 119: . 287 004 IHC; tested species: mouse
P75 neurotrophin receptor controls subventricular zone neural stem cell migration after stroke.
Deshpande SS, Malik SC, Conforti P, Lin JD, Chu YH, Nath S, Greulich F, Dumbach MA, Uhlenhaut NH, Schachtrup C
Cell and tissue research (2021) : . 287 004 IHC; tested species: mouse
Gamma-Aminobutyric Acid Transporters in the Nucleus Tractus Solitarii Regulate Inhibitory and Excitatory Synaptic Currents That Influence Cardiorespiratory Function.
Martinez D, Lima-Silveira L, Matott MP, Hasser EM, Kline DD
Frontiers in physiology (2021) 12: 821110. 287 004 IHC; tested species: rat
GSK-3β S9A overexpression leads murine hippocampal neural precursors to acquire an astroglial phenotype in vivo.
Flor-García M, Ávila J, Llorens-Martín M
Developmental neurobiology (2021) 815: 710-723. 287 004 IHC; tested species: mouse
Influence of Tilia tomentosa Moench Extract on Mouse Small Intestine Neuromuscular Contractility.
Cerantola S, Faggin S, Annaloro G, Mainente F, Filippini R, Savarino EV, Piovan A, Zoccatelli G, Giron MC
Nutrients (2021) 1310: . 287 004 IHC; tested species: mouse
A central role for anterior cingulate cortex in the control of pathological aggression.
van Heukelum S, Tulva K, Geers FE, van Dulm S, Ruisch IH, Mill J, Viana JF, Beckmann CF, Buitelaar JK, Poelmans G, Glennon JC, et al.
Current biology : CB (2021) 3111: 2321-2333.e5. 287 004 IHC; tested species: mouse
Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice.
Schob S, Puchta J, Winter K, Michalski D, Mages B, Martens H, Emmer A, Hoffmann KT, Gaunitz F, Meinicke A, Krause M, et al.
Journal of chemical neuroanatomy (2021) 118: 102036. 287 004 IHC; tested species: mouse
Astrocyte-derived neurons provide excitatory input to the adult striatal circuitry.
Dorst MC, Díaz-Moreno M, Dias DO, Guimarães EL, Holl D, Kalkitsas J, Silberberg G, Göritz C
Proceedings of the National Academy of Sciences of the United States of America (2021) 11833: . 287 004 IHC; tested species: mouse
Structural Degradation in Midcingulate Cortex Is Associated with Pathological Aggression in Mice.
Heukelum SV, Geers FE, Tulva K, van Dulm S, Beckmann CF, Buitelaar JK, Glennon JC, Vogt BA, Havenith MN, França ASC
Brain sciences (2021) 117: . 287 004 IHC; tested species: mouse
Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis.
Lee JH, Kim JY, Noh S, Lee H, Lee SY, Mun JY, Park H, Chung WS
Nature (2021) 5907847: 612-617. 287 004 IHC; tested species: mouse
Hippocampal neurons respond to brain activity with functional hypoxia.
Butt UJ, Steixner-Kumar AA, Depp C, Sun T, Hassouna I, Wüstefeld L, Arinrad S, Zillmann MR, Schopf N, Fernandez Garcia-Agudo L, Mohrmann L, et al.
Molecular psychiatry (2021) : . 287 004 IHC; tested species: mouse
S100β-mediated astroglial control of firing and input processing in layer 5 pyramidal neurons of the mouse visual cortex.
Ryczko D, Hanini-Daoud M, Condamine S, Bréant BJB, Fougère M, Araya R, Kolta A
The Journal of physiology (2020) : . 287 004 IHC; tested species: mouse
GABA uptake transporters support dopamine release in dorsal striatum with maladaptive downregulation in a parkinsonism model.
Roberts BM, Doig NM, Brimblecombe KR, Lopes EF, Siddorn RE, Threlfell S, Connor-Robson N, Bengoa-Vergniory N, Pasternack N, Wade-Martins R, Magill PJ, et al.
Nature communications (2020) 111: 4958. 287 004 IHC; tested species: mouse
Astroglial dysfunctions drive aberrant synaptogenesis and social behavioral deficits in mice with neonatal exposure to lengthy general anesthesia.
Zhou B, Chen L, Liao P, Huang L, Chen Z, Liao D, Yang L, Wang J, Yu G, Wang L, Zhang J, et al.
PLoS biology (2019) 178: e3000086. 287 004 IHC; tested species: mouse
AGO CLIP Reveals an Activated Network for Acute Regulation of Brain Glutamate Homeostasis in Ischemic Stroke.
Kobayashi M, Benakis C, Anderson C, Moore MJ, Poon C, Uekawa K, Dyke JP, Fak JJ, Mele A, Park CY, Zhou P, et al.
Cell reports (2019) 284: 979-991.e6. 287 004 IHC; tested species: mouse
Radial glial elements in the cerebral cortex of the lesser hedgehog tenrec.
Mack AF, Künzle H, Lange M, Mages B, Reichenbach A, Härtig W
Brain structure & function (2018) : . 287 004 IHC
Glucocorticoid receptor signaling in astrocytes is required for aversive memory formation.
Tertil M, Skupio U, Barut J, Dubovyk V, Wawrzczak-Bargiela A, Soltys Z, Golda S, Kudla L, Wiktorowska L, Szklarczyk K, Korostynski M, et al.
Translational psychiatry (2018) 81: 255. 287 004 IHC; tested species: mouse
Parvalbumin-expressing ependymal cells in rostral lateral ventricle wall adhesions contribute to aging-related ventricle stenosis in mice.
Filice F, Celio MR, Babalian A, Blum W, Szabolcsi V
The Journal of comparative neurology (2017) 52515: 3266-3285. 287 004 IHC, WB; tested species: mouse
CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.
Woelfle S, Deshpande D, Feldengut S, Braak H, Del Tredici K, Roselli F, Deisseroth K, Michaelis J, Boeckers TM, Schön M
BMC biology (2023) 211: 113. 287 004 Clarity; tested species: human
ICC
IHC
IHC-P
IHC-G
IHC-G
Background

The family of S100 proteins comprises more than 20 members. These proteins are EF-hand Ca2+-binding proteins, and are widely distributed in mammalian tissue. Since these proteins are soluble in 100 % saturated ammonium-sulfate solution they have been named S100.
S100B is a frequently used marker protein for mature astrocytes whereas GFAP is also expressed in germinal zone cells that maintained their immature developmental stage.
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