Synaptic Systems

Cat. No.: 287 003

Amount: 50 µg

Price: $375.00

Cat. No. 287 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 1000 up to 1 : 2000 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 92 from rat S100B (UniProt Id: P04631)
Reactivity	Reacts with: rat (P04631), mouse (P50114). Other species not tested yet.
Data sheet	287_003.pdf

References for S100B - 287 003

A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ.
Heller JP, Rusakov DA
Methods in molecular biology (Clifton, N.J.) (2019) 1938: 69-84. 287 003 ICC; tested species: rat

Probing nano-organization of astroglia with multi-color super-resolution microscopy.
Heller JP, Michaluk P, Sugao K, Rusakov DA
Journal of neuroscience research (2017) 9511: 2159-2171. 287 003 ICC; tested species: rat

Mapping multi-regional functional connectivity of astrocyte-neuronal networks during behaviors.
Wang H, Huang M, Yang S, Xu J, Li J, Qin H, Liang S, Teng T, Yang C, Gong M, He Y, et al.
Neurophotonics (2024) 114: 045010. 287 003 IHC; tested species: mouse

Evolutionary changes leading to efficient glymphatic circulation in the mammalian brain.
Kameya N, Sakai I, Saito K, Hamabe-Horiike T, Shinmyo Y, Nakada M, Okuda S, Kawasaki H
Nature communications (2024) 151: 10048. 287 003 IHC

Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens.
Serra I, Esparza J, Delgado L, Martín-Monteagudo C, Puigròs M, Podlesniy P, Trullás R, Navarrete M
Nature communications (2022) 131: 5272. 287 003 IHC; tested species: mouse

Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Schiweck J, Murk K, Ledderose J, Münster-Wandowski A, Ornaghi M, Vida I, Eickholt BJ
Nature communications (2021) 121: 1490. 287 003 IHC; tested species: mouse

MeCP2 controls neural stem cell fate specification through miR-199a-mediated inhibition of BMP-Smad signaling.
Nakashima H, Tsujimura K, Irie K, Imamura T, Trujillo CA, Ishizu M, Uesaka M, Pan M, Noguchi H, Okada K, Aoyagi K, et al.
Cell reports (2021) 357: 109124. 287 003 IHC; tested species: human,mouse

Glial A2B Adenosine Receptors Modulate Abnormal Tachykininergic Responses and Prevent Enteric Inflammation Associated with High Fat Diet-Induced Obesity.
D'Antongiovanni V, Benvenuti L, Fornai M, Pellegrini C, van den Wijngaard R, Cerantola S, Giron MC, Caputi V, Colucci R, Haskó G, Németh ZH, et al.
Cells (2020) 95: . 287 003 IHC; tested species: mouse

FGF signaling directs the cell fate switch from neurons to astrocytes in the developing mouse cerebral cortex.
Anh Dinh Duong T, Hoshiba Y, Saito K, Kawasaki K, Ichikawa Y, Matsumoto N, Shinmyo Y, Kawasaki H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 287 003 IHC; tested species: mouse

Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 287 003 IHC; tested species: mouse

Optimized Protocol for Proportionate CNS Cell Retrieval as a Versatile Platform for Cellular and Molecular Phenomapping in Aging and Neurodegeneration.
Ain Q, Schmeer CW, Wengerodt D, Hofmann Y, Witte OW, Kretz A
International journal of molecular sciences (2022) 236: . 287 003 FACS; tested species: mouse

Cat. No.: 287 003

Amount: 50 µg

Price: $375.00

ICC

IHC

IHC-P

Background

The family of S100 proteins comprises more than 20 members. These proteins are EF-hand Ca²⁺-binding proteins, and are widely distributed in mammalian tissue. Since these proteins are soluble in 100 % saturated ammonium-sulfate solution they have been named S100.
S100B is a frequently used marker protein for mature astrocytes whereas GFAP is also expressed in germinal zone cells that maintained their immature developmental stage.

1 ) Gos T et al., J Psychiatr Res (2013)
2 ) Ondruschka B et al., J. Neurotrauma (2013)
3 ) Raponi E et al., Glia (2007)
4 ) Lin J et al., J. Biol. Chem. (2004)

Protocols

ICC

IHC

IHC-P

Other products for this target

S100B antibody - 287 003

References for S100B - 287 003