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S100B antibody - 287 003

S100B is a marker for mature astrocytes
Rabbit polyclonal purified antibody
Cat. No.: 287 003
Amount: 50 µg
Price: $370.00
Cat. No. 287 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 2000 gallery  
IHC-P: 1 : 200 gallery  
Immunogen Recombinant protein corresponding to AA 1 to 92 from rat S100B (UniProt Id: P04631)
Reactivity Reacts with: rat (P04631), mouse (P50114).
Other species not tested yet.
Specificity Specific for S100B
Data sheet 287_003.pdf

References for S100B - 287 003

A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ.
Heller JP, Rusakov DA
Methods in molecular biology (Clifton, N.J.) (2019) 1938: 69-84. 287 003 ICC; tested species: rat
Probing nano-organization of astroglia with multi-color super-resolution microscopy.
Heller JP, Michaluk P, Sugao K, Rusakov DA
Journal of neuroscience research (2017) 9511: 2159-2171. 287 003 ICC; tested species: rat
Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens.
Serra I, Esparza J, Delgado L, Martín-Monteagudo C, Puigròs M, Podlesniy P, Trullás R, Navarrete M
Nature communications (2022) 131: 5272. 287 003 IHC; tested species: mouse
Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Schiweck J, Murk K, Ledderose J, Münster-Wandowski A, Ornaghi M, Vida I, Eickholt BJ
Nature communications (2021) 121: 1490. 287 003 IHC; tested species: mouse
MeCP2 controls neural stem cell fate specification through miR-199a-mediated inhibition of BMP-Smad signaling.
Nakashima H, Tsujimura K, Irie K, Imamura T, Trujillo CA, Ishizu M, Uesaka M, Pan M, Noguchi H, Okada K, Aoyagi K, et al.
Cell reports (2021) 357: 109124. 287 003 IHC; tested species: human,mouse
Glial A2B Adenosine Receptors Modulate Abnormal Tachykininergic Responses and Prevent Enteric Inflammation Associated with High Fat Diet-Induced Obesity.
D'Antongiovanni V, Benvenuti L, Fornai M, Pellegrini C, van den Wijngaard R, Cerantola S, Giron MC, Caputi V, Colucci R, Haskó G, Németh ZH, et al.
Cells (2020) 95: . 287 003 IHC; tested species: mouse
FGF signaling directs the cell fate switch from neurons to astrocytes in the developing mouse cerebral cortex.
Anh Dinh Duong T, Hoshiba Y, Saito K, Kawasaki K, Ichikawa Y, Matsumoto N, Shinmyo Y, Kawasaki H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 287 003 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 287 003 IHC; tested species: mouse
Cat. No.: 287 003
Amount: 50 µg
Price: $370.00
A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ.
Heller JP, Rusakov DA
Methods in molecular biology (Clifton, N.J.) (2019) 1938: 69-84. 287 003 ICC; tested species: rat
Probing nano-organization of astroglia with multi-color super-resolution microscopy.
Heller JP, Michaluk P, Sugao K, Rusakov DA
Journal of neuroscience research (2017) 9511: 2159-2171. 287 003 ICC; tested species: rat
Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens.
Serra I, Esparza J, Delgado L, Martín-Monteagudo C, Puigròs M, Podlesniy P, Trullás R, Navarrete M
Nature communications (2022) 131: 5272. 287 003 IHC; tested species: mouse
Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Schiweck J, Murk K, Ledderose J, Münster-Wandowski A, Ornaghi M, Vida I, Eickholt BJ
Nature communications (2021) 121: 1490. 287 003 IHC; tested species: mouse
MeCP2 controls neural stem cell fate specification through miR-199a-mediated inhibition of BMP-Smad signaling.
Nakashima H, Tsujimura K, Irie K, Imamura T, Trujillo CA, Ishizu M, Uesaka M, Pan M, Noguchi H, Okada K, Aoyagi K, et al.
Cell reports (2021) 357: 109124. 287 003 IHC; tested species: human,mouse
Glial A2B Adenosine Receptors Modulate Abnormal Tachykininergic Responses and Prevent Enteric Inflammation Associated with High Fat Diet-Induced Obesity.
D'Antongiovanni V, Benvenuti L, Fornai M, Pellegrini C, van den Wijngaard R, Cerantola S, Giron MC, Caputi V, Colucci R, Haskó G, Németh ZH, et al.
Cells (2020) 95: . 287 003 IHC; tested species: mouse
FGF signaling directs the cell fate switch from neurons to astrocytes in the developing mouse cerebral cortex.
Anh Dinh Duong T, Hoshiba Y, Saito K, Kawasaki K, Ichikawa Y, Matsumoto N, Shinmyo Y, Kawasaki H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 287 003 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 287 003 IHC; tested species: mouse
Background

The family of S100 proteins comprises more than 20 members. These proteins are EF-hand Ca2+-binding proteins, and are widely distributed in mammalian tissue. Since these proteins are soluble in 100 % saturated ammonium-sulfate solution they have been named S100.
S100B is a frequently used marker protein for mature astrocytes whereas GFAP is also expressed in germinal zone cells that maintained their immature developmental stage.