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Home > Products > 276 003

EPB41 (4.1R) antibody - 276 003 K.D.

EPB41 is a member of the 4.1 protein family
Rabbit polyclonal purified antibody
Cat. No.: 276 003
Amount: 50 µg
Price: $375.00
Cat. No. 276 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 200 up to 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 50000 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 180 from mouse 4.1R (UniProt Id: P48193)
Reactivity Reacts with: rat (A0A0G2K161), mouse (P48193).
Other species not tested yet.
Specificity K.D. validated
Data sheet 276_003.pdf
Cat. No.: 276 003
Amount: 50 µg
Price: $375.00
WB
ICC
IHC
Background

The protein 4.1 family consists of a group of skeletal proteins related to the red blood cell (RBC) protein 4.1R, also known as the erythrocyte membrane skeletal band 4.1 protein EPB 41. This protein family also includes 4.1N (EPB 4), 4.1B and 4.1G (EPB 4.1L2).
4.1N and 4.1G are expressed in neuronal and non-neuronal cells in the brain. For 4.1N several splice variants with tissue specific expression patterns have been described. The 135 kDa isoform is most prominent in brain whereas a smaller 100 kDa variant is enriched in peripheral tissues