Synaptic Systems - GABA transporter1

Cat. No.: 274 104

Amount: 100 µl

Price: $365.00

Cat. No. 274 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 585 to 599 from mouse GABA transporter1 (UniProt Id: P31648)
Reactivity	Reacts with: rat (P23978), mouse (P31648). Other species not tested yet.
Matching control protein/peptide	274-1P
Remarks	WB: GABA transporter 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Data sheet	274_104.pdf

References for GABA transporter1 - 274 104

Oligodendrocyte Differentiation and Myelination Is Potentiated via GABAB Receptor Activation.
Serrano-Regal MP, Luengas-Escuza I, Bayón-Cordero L, Ibarra-Aizpurua N, Alberdi E, Pérez-Samartín A, Matute C, Sánchez-Gómez MV
Neuroscience (2019) : . 274 104 ICC, IHC; tested species: rat

GAT-1 mediated GABA uptake in rat oligodendrocytes.
Fattorini G, Melone M, Sánchez-Gómez MV, Arellano RO, Bassi S, Matute C, Conti F
Glia (2017) 653: 514-522. 274 104 IHC; tested species: rat

Cat. No.: 274 104

Amount: 100 µl

Price: $365.00

GAT-1 mediated GABA uptake in rat oligodendrocytes.
Fattorini G, Melone M, Sánchez-Gómez MV, Arellano RO, Bassi S, Matute C, Conti F
Glia (2017) 653: 514-522. 274 104 IHC; tested species: rat

ICC

IHC

IHC-P

Background

γ-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. After the release of GABA from synaptic vesicles into the synaptic cleft during neurotransmission, GABA transporters (GATs) remove extracellular GABA by reuptake into the presynaptic terminal.
Three GABA transporters are described so far of which only GAT 1 and GAT 3 are expressed in the brain.

1 ) Hu J et al., Neuropharmacology (2008)
2 ) Quick MW et al., J. Biol. Chem. (2004)
3 ) Law RM et al., J. Biol. Chem. (2000)
4 ) Beckman ML et al., J. Neurosci. (1998)
5 ) Ikegaki N et al., Brain Res. Mol. Brain Res. (1994)
6 ) Jursky F et al., J. Exp. Biol. (1994)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

GABA transporter1 antibody - 274 104

References for GABA transporter1 - 274 104