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GABA transporter 1 antibody - 274 104

GATs are neurotransmitter transporters involved in the termination of GABA transmission
Polyclonal Guinea pig antiserum
Cat. No.: 274 104
Amount: 100 µl
Price: $350.00
Cat. No. 274 104 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 585 to 599 from mouse GABA transporter1 (UniProt Id: P31648)
Reactivity Reacts with: rat (P23978), mouse (P31648).
Other species not tested yet.
Specificity Specific for GAT 1.
Matching control protein/peptide 274-1P
Remarks

WB:  GABA transporter 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).

Data sheet 274_104.pdf

References for GABA transporter 1 - 274 104

Oligodendrocyte Differentiation and Myelination Is Potentiated via GABAB Receptor Activation.
Serrano-Regal MP, Luengas-Escuza I, Bayón-Cordero L, Ibarra-Aizpurua N, Alberdi E, Pérez-Samartín A, Matute C, Sánchez-Gómez MV
Neuroscience (2019) : . 274 104 ICC, IHC; tested species: rat
Oligodendrocyte Differentiation and Myelination Is Potentiated via GABAB Receptor Activation.
Serrano-Regal MP, Luengas-Escuza I, Bayón-Cordero L, Ibarra-Aizpurua N, Alberdi E, Pérez-Samartín A, Matute C, Sánchez-Gómez MV
Neuroscience (2019) : . 274 104 ICC, IHC; tested species: rat
GAT-1 mediated GABA uptake in rat oligodendrocytes.
Fattorini G, Melone M, Sánchez-Gómez MV, Arellano RO, Bassi S, Matute C, Conti F
Glia (2017) 653: 514-522. 274 104 IHC; tested species: rat
Cat. No.: 274 104
Quantity: 100 µl
Price: $350.00
Oligodendrocyte Differentiation and Myelination Is Potentiated via GABAB Receptor Activation.
Serrano-Regal MP, Luengas-Escuza I, Bayón-Cordero L, Ibarra-Aizpurua N, Alberdi E, Pérez-Samartín A, Matute C, Sánchez-Gómez MV
Neuroscience (2019) : . 274 104 ICC, IHC; tested species: rat
Oligodendrocyte Differentiation and Myelination Is Potentiated via GABAB Receptor Activation.
Serrano-Regal MP, Luengas-Escuza I, Bayón-Cordero L, Ibarra-Aizpurua N, Alberdi E, Pérez-Samartín A, Matute C, Sánchez-Gómez MV
Neuroscience (2019) : . 274 104 ICC, IHC; tested species: rat
GAT-1 mediated GABA uptake in rat oligodendrocytes.
Fattorini G, Melone M, Sánchez-Gómez MV, Arellano RO, Bassi S, Matute C, Conti F
Glia (2017) 653: 514-522. 274 104 IHC; tested species: rat
Background
γ-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. After the release of GABA from synaptic vesicles into the synaptic cleft during neurotransmission, GABA transporters (GATs) remove extracellular GABA by reuptake into the presynaptic terminal.
Three GABA transporters are described so far of which only GAT 1 and GAT 3 are expressed in the brain.