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Home > Products > 274 102

GABA transporter1 antibody - 274 102 K.O.

GATs are neurotransmitter transporters involved in the termination of GABA transmission
Rabbit polyclonal antiserum
Cat. No.: 274 102
Amount: 200 µl
Price: $360.00
Cat. No. 274 102 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  
ExM: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen Synthetic peptide corresponding to AA 585 to 599 from mouse GABA transporter1 (UniProt Id: P31648)
Reactivity Reacts with: rat (P23978), mouse (P31648).
Other species not tested yet.
Specificity K.O. validated PubMed: 33664860
Matching control protein/peptide 274-1P
Remarks

WB:  GABA transporter 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 274_102.pdf

References for GABA transporter1 - 274 102

GABA uptake transporters support dopamine release in dorsal striatum with maladaptive downregulation in a parkinsonism model.
Roberts BM, Doig NM, Brimblecombe KR, Lopes EF, Siddorn RE, Threlfell S, Connor-Robson N, Bengoa-Vergniory N, Pasternack N, Wade-Martins R, Magill PJ, et al.
Nature communications (2020) 111: 4958. 274 102 WB, IHC; tested species: mouse
A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles.
Liang D, Zhou L, Zhou H, Zhang F, Fang G, Leng J, Wu Y, Zhang Y, Yang A, Liu Y, Chen YH, et al.
Cell research (2024) : . 274 102 ICC, IHC; tested species: mouse
A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles.
Liang D, Zhou L, Zhou H, Zhang F, Fang G, Leng J, Wu Y, Zhang Y, Yang A, Liu Y, Chen YH, et al.
Cell research (2024) : . 274 102 ICC, IHC; tested species: mouse
Gamma-Aminobutyric Acid Transporters in the Nucleus Tractus Solitarii Regulate Inhibitory and Excitatory Synaptic Currents That Influence Cardiorespiratory Function.
Martinez D, Lima-Silveira L, Matott MP, Hasser EM, Kline DD
Frontiers in physiology (2021) 12: 821110. 274 102 IHC; tested species: rat
Environmental enrichment implies GAT-1 as a potential therapeutic target for stroke recovery.
Lin Y, Yao M, Wu H, Wu F, Cao S, Ni H, Dong J, Yang D, Sun Y, Kou X, Li J, et al.
Theranostics (2021) 118: 3760-3780. 274 102 IHC; KO verified; tested species: mouse
GABA uptake transporters support dopamine release in dorsal striatum with maladaptive downregulation in a parkinsonism model.
Roberts BM, Doig NM, Brimblecombe KR, Lopes EF, Siddorn RE, Threlfell S, Connor-Robson N, Bengoa-Vergniory N, Pasternack N, Wade-Martins R, Magill PJ, et al.
Nature communications (2020) 111: 4958. 274 102 WB, IHC; tested species: mouse
Structural Heterogeneity of the GABAergic Tripartite Synapse.
Brunskine C, Passlick S, Henneberger C
Cells (2022) 1119: . 274 102 ExM; tested species: mouse
Cat. No.: 274 102
Amount: 200 µl
Price: $360.00
GABA uptake transporters support dopamine release in dorsal striatum with maladaptive downregulation in a parkinsonism model.
Roberts BM, Doig NM, Brimblecombe KR, Lopes EF, Siddorn RE, Threlfell S, Connor-Robson N, Bengoa-Vergniory N, Pasternack N, Wade-Martins R, Magill PJ, et al.
Nature communications (2020) 111: 4958. 274 102 WB, IHC; tested species: mouse
A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles.
Liang D, Zhou L, Zhou H, Zhang F, Fang G, Leng J, Wu Y, Zhang Y, Yang A, Liu Y, Chen YH, et al.
Cell research (2024) : . 274 102 ICC, IHC; tested species: mouse
A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles.
Liang D, Zhou L, Zhou H, Zhang F, Fang G, Leng J, Wu Y, Zhang Y, Yang A, Liu Y, Chen YH, et al.
Cell research (2024) : . 274 102 ICC, IHC; tested species: mouse
Gamma-Aminobutyric Acid Transporters in the Nucleus Tractus Solitarii Regulate Inhibitory and Excitatory Synaptic Currents That Influence Cardiorespiratory Function.
Martinez D, Lima-Silveira L, Matott MP, Hasser EM, Kline DD
Frontiers in physiology (2021) 12: 821110. 274 102 IHC; tested species: rat
Environmental enrichment implies GAT-1 as a potential therapeutic target for stroke recovery.
Lin Y, Yao M, Wu H, Wu F, Cao S, Ni H, Dong J, Yang D, Sun Y, Kou X, Li J, et al.
Theranostics (2021) 118: 3760-3780. 274 102 IHC; KO verified; tested species: mouse
GABA uptake transporters support dopamine release in dorsal striatum with maladaptive downregulation in a parkinsonism model.
Roberts BM, Doig NM, Brimblecombe KR, Lopes EF, Siddorn RE, Threlfell S, Connor-Robson N, Bengoa-Vergniory N, Pasternack N, Wade-Martins R, Magill PJ, et al.
Nature communications (2020) 111: 4958. 274 102 WB, IHC; tested species: mouse
Structural Heterogeneity of the GABAergic Tripartite Synapse.
Brunskine C, Passlick S, Henneberger C
Cells (2022) 1119: . 274 102 ExM; tested species: mouse
WB
ICC
IHC
IHC-P
Background
γ-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. After the release of GABA from synaptic vesicles into the synaptic cleft during neurotransmission, GABA transporters (GATs) remove extracellular GABA by reuptake into the presynaptic terminal.
Three GABA transporters are described so far of which only GAT 1 and GAT 3 are expressed in the brain.
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