NeuN antibody - 266 108

NeuN is a neuron-specific nuclear protein in vertebrates

Rabbit monoclonal recombinant IgG

Cat. No.: 266 108

Amount: 50 µg

Price: $415.00

Cat. No. 266 108	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb167H3
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino terminus of human NeuN (UniProt Id: A6NFN3)
Reactivity	Reacts with: mouse (Q8BIF2), rat (D4A2H6), human (A6NFN3). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody SY-167H3. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	266_108.pdf

Cat. No.: 266 108

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

Background

NeuN (Neuronal Nuclei) is a neuron-specific nuclear protein that has recently been identified as Fox-3/Rbfox3, a member of the Fox-1 family of transcription factors.
NeuN is only expressed in the nuclei of differentiated neurons. In some neurons - Purkinje cells, sympathetic ganglion cells, INL retinal cells, Cajal-Retzius cells, inferior olivary and dentate nucleus neurons - NeuN is not detectable.

1 ) Morin LP et al., Brain Res. (2011)
2 ) Dent MA et al., FEBS Lett. (2010)
3 ) Cannon JR et al., Neurosci. Lett. (2009)
4 ) Kim KK et al., J. Biol. Chem. (2009)
5 ) Mullen RJ et al., Development (1992)

Protocols

ICC

IHC

IHC-P

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