Tailor-made Antibodies
and Tools for Life Science

TEV-cut site antibody - 265 003CpH

TEV is a cysteine protease frequently used in biochemistry

pH-sensitive probe fluorescent only under acidic conditions

Rabbit polyclonal purified antibody
Cat. No.: 265 003CpH
Amount: 50 µg
Price: $485.00
Cat. No. 265 003CpH 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
Label CypHer5E
Immunogen Synthetic peptide corresponding to AA 1 to 6 from TEV-cut site
Specificity TEV-cut protease site is recognized with very strong preference.
Remarks

Excess of extracellular membrane bound SynaptoPhluorin is often removed by TEV protease digestion. This antibody recognizes the neoepitopes generated by TEV cleavage and can be used for membrane internalization studies. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.

Data sheet 265_003cph.pdf
Cat. No.: 265 003CpH
Amount: 50 µg
Price: $485.00