Synaptic Systems

Cat. No.: 263 005

Amount: 50 µg

Price: $460.00

Cat. No. 263 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 427 from rat Giantin (UniProt Id: G3V6A8)
Reactivity	Reacts with: rat, mouse. Other species not tested yet.
Remarks	WB: Due to its large size, Giantin requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Data sheet	263_005.pdf

References for Giantin - 263 005

A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 263 005 ICC; tested species: human

The X-Linked Intellectual Disability Gene Zdhhc9 Is Essential for Dendrite Outgrowth and Inhibitory Synapse Formation.
Shimell JJ, Shah BS, Cain SM, Thouta S, Kuhlmann N, Tatarnikov I, Jovellar DB, Brigidi GS, Kass J, Milnerwood AJ, Snutch TP, et al.
Cell reports (2019) 298: 2422-2437.e8. 263 005 ICC; tested species: rat

Cat. No.: 263 005

Amount: 50 µg

Price: $460.00

ICC

IHC

IHC-P

Background

In eukaryotic cells the Golgi complex consists of stacked cisternae. Giantin, also referred to as macrogolgin or p400, is a single pass membrane protein involved in tethering these cisternae by cross-bridge formation. Its large cytoplasmic N-terminus is involved in the docking of COPI vesicles via p115 to the Golgi membrane.
Systemic lupus erythematosus and Sjögren's syndrome correlate with autoimmune antibodies against Golgi-compartment proteins with giantin being the most common autoantigen.

1 ) Rosing M et al., Exp. Cell Res. (2007)
2 ) Nozawa K et al., Arthritis Res. Ther. (2004)
3 ) Alvarez C et al., J. Biol. Chem. (2001)
4 ) Sönnichsen B et al., J. Cell Biol. (1998)
5 ) Seelig HP et al., Mol. Cell. Biol. (1994)
6 ) Linstedt AD et al., Mol. Biol. Cell (1993)

Protocols

ICC

IHC

IHC-P

Other products for this target

Giantin antibody - 263 005

References for Giantin - 263 005