Synaptic Systems - Collybistin

Cat. No.: 261 003

Amount: 50 µg

Price: $380.00

Cat. No. 261 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 gallery IP: yes ICC: 1 : 500 gallery IHC: yes IHC-P: not tested yet
Immunogen	Recombinant protein corresponding to AA 4 to 229 from mouse Collybistin (UniProt Id: Q3UTH8)
Reactivity	Reacts with: rat (Q9QX73), mouse (Q3UTH8). Other species not tested yet.
Specificity	Specific for collybistin; immunogen present in all three described splice-variants K.O. validated
Data sheet	261_003.pdf

miRNA-mediated control of gephyrin synthesis drives sustained inhibitory synaptic plasticity.
Welle TM, Rajgor D, Kareemo DJ, Garcia JD, Zych SM, Wolfe SE, Gookin SE, Martinez TP, Dell'Acqua ML, Ford CP, Kennedy MJ, et al.
EMBO reports (2024) 2511: 5141-5168. 261 003 WB; tested species: rat

A proline-rich motif in the large intracellular loop of the glycine receptor α1 subunit interacts with the Pleckstrin homology domain of collybistin.
Breitinger U, Weinländer K, Pechmann Y, Langlhofer G, Enz R, Becker CM, Sticht H, Kneussel M, Villmann C, Breitinger HG
Journal of advanced research (2021) 29: 95-106. 261 003 WB, ICC; tested species: mouse

Phosphorylation on Ser 359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses.
Nakamura Y, Morrow DH, Nathanson AJ, Henley JM, Wilkinson KA, Moss SJ
The Journal of biological chemistry (2020) : . 261 003 WB; tested species: rat

Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 261 003 WB, ICC; tested species: mouse

Collybistin binds and inhibits mTORC1 signaling: a potential novel mechanism contributing to intellectual disability and autism.
Machado CO, Griesi-Oliveira K, Rosenberg C, Kok F, Martins S, Passos-Bueno MR, Sertie AL
European journal of human genetics : EJHG (2016) 241: 59-65. 261 003 WB

Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged γ-Aminobutyric Acid Receptor, Type A (GABAA), α2 Subunit Knock-in Mouse.
Nakamura Y, Morrow DH, Modgil A, Huyghe D, Deeb TZ, Lumb MJ, Davies PA, Moss SJ
The Journal of biological chemistry (2016) 29123: 12394-407. 261 003 WB

Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters.
Ye R, Quinlan MA, Iwamoto H, Wu HH, Green NH, Jetter CS, McMahon DG, Veestra-VanderWeele J, Levitt P, Blakely RD
Frontiers in synaptic neuroscience (2015) 7: 20. 261 003 WB

A conformational switch in collybistin determines the differentiation of inhibitory postsynapses.
Soykan T, Schneeberger D, Tria G, Buechner C, Bader N, Svergun D, Tessmer I, Poulopoulos A, Papadopoulos T, Varoqueaux F, Schindelin H, et al.
The EMBO journal (2014) 3318: 2113-33. 261 003 WB

Cat. No.: 261 003

Amount: 50 µg

Price: $380.00

ICC

Background

Protocols

Collybistin antibody - 261 003 K.O.