NET antibody - 260 003

The NE transporter is involved in the termination of synaptic transmission

Rabbit polyclonal purified antibody

Cat. No.: 260 003

Amount: 50 µg

Price: $375.00

Cat. No. 260 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 189 to 204 from rat NET (UniProt Id: Q63380)
Reactivity	Reacts with: rat (Q63380), mouse (O55192). Other species not tested yet.
Matching control protein/peptide	260-0P
Data sheet	260_003.pdf

References for NET - 260 003

Lack of Cannabinoid Receptor Type-1 Leads to Enhanced Age-Related Neuronal Loss in the Locus Coeruleus.
Gargano A, Beins E, Zimmer A, Bilkei-Gorzo A
International journal of molecular sciences (2020) 221: . 260 003 IHC; tested species: mouse

Structural basis for noradrenergic regulation of neural circuits in the mouse olfactory bulb.
Horie S, Kiyokage E, Hayashi S, Inoue K, Sohn J, Hioki H, Furuta T, Toida K
The Journal of comparative neurology (2020) : . 260 003 IHC; tested species: mouse

Cat. No.: 260 003

Amount: 50 µg

Price: $375.00

IHC

Background

The norepinephrine (NE) transporter (NET), also referred to as Sodium-dependent noradrenaline transporter, is expressed on the plasma membranes of noradrenergic neurons. It is responsible for the inactivation of synaptically released NE and is a target for many antidepressants and psychostimulants.

1 ) Robertson SD et al., J. Neurosci. (2010)
2 ) Li Z et al., J. Neurosci. (2010)
3 ) Guiard BP et al., Mol. Pharmacol. (2008)
4 ) Schroeter S et al., J. Comp. Neurol. (2000)
5 ) Kuczenski R et al., J. Neurosci. (1995)

Protocols

IHC

OTHER

Preabsorbtion

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