Synaptic Systems - Cannabinoid receptor CB1-R

Cat. No.: 258 104

Amount: 100 µl

Price: $370.00

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Cat. No. 258 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of rat CB1-R (UniProt Id: P20272)
Reactivity	Reacts with: rat (P20272), mouse (P47746). Other species not tested yet.
Data sheet	Data Sheet 258_104 Safety Data Sheet (SDS)

References for Cannabinoid receptor CB1-R - 258 104

Inhibitory CCK+ basket synapse defects in mouse models of dystroglycanopathy.
Jahncke JN, Miller DS, Krush M, Schnell E, Wright KM
eLife (2024) 12: . 258 104 IHC; tested species: mouse

The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus.
Wen L, Yang X, Wu Z, Fu S, Zhan Y, Chen Z, Bi D, Shen Y
Cell reports (2023) 424: 112349. 258 104 IHC; tested species: mouse

Neuronal Dystroglycan regulates postnatal development of CCK/cannabinoid receptor-1 interneurons.
Miller DS, Wright KM
Neural development (2021) 161: 4. 258 104 IHC; tested species: mouse

Cat. No.: 258 104

Amount: 100 µl

Price: $370.00

Inhibitory CCK+ basket synapse defects in mouse models of dystroglycanopathy.
Jahncke JN, Miller DS, Krush M, Schnell E, Wright KM
eLife (2024) 12: . 258 104 IHC; tested species: mouse

Neuronal Dystroglycan regulates postnatal development of CCK/cannabinoid receptor-1 interneurons.
Miller DS, Wright KM
Neural development (2021) 161: 4. 258 104 IHC; tested species: mouse

ICC
(1 img)

IHC
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IHC-P (FFPE)
(1 img)

Background

The cannabinoid receptor CB1-R is a G-protein coupled receptor (GPCR) with 7 transmembrane domains. It is responsive to tetrahydrocannabinol, the psychotropic component of marijuana.
Endogenous cannabinoids (endocannabinoids) are released from postsynaptic neurons and act onto presynaptic cannabinoid receptors where they play important physiological roles in synaptic plasticity, analgesia, appetite, and neuroprotection.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Osborne KD et al., ASN Neuro (2009)
2 ) Niehaus JL et al., Mol. Pharmacol. (2007)
3 ) Kawamura Y et al., J. Neurosci. (2006)
4 ) Shire D et al., J. Biol. Chem. (1995)
5 ) Matsuda LA et al., Nature (1990)
6 ) Howlett AC et al., Mol. Pharmacol. (1985)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

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Cannabinoid receptor CB1-R antibody - 258 104

References for Cannabinoid receptor CB1-R - 258 104