Synaptic Systems - Cannabinoid receptor CB1-R

Cat. No.: 258 011

Amount: 100 µg

Price: $425.00

Cat. No. 258 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery
Clone	289C1
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 450 to 473 from rat CB1-R (UniProt Id: P20272)
Reactivity	Reacts with: rat (P20272), mouse (P47746), human (P21554). Other species not tested yet.
Specificity	K.O. validated
Data sheet	258_011.pdf

References for Cannabinoid receptor CB1-R - 258 011

WB (1)
ICC (1)
IHC (13)

Impact of the mouse estrus cycle on cannabinoid receptor agonist-induced molecular and behavioral outcomes.
Kim HJJ, Zagzoog A, Black T, Baccetto SL, Ezeaka UC, Laprairie RB
Pharmacology research & perspectives (2022) 103: e00950. 258 011 WB; tested species: mouse

Cannabidiol modulates excitatory-inhibitory ratio to counter hippocampal hyperactivity.
Rosenberg EC, Chamberland S, Bazelot M, Nebet ER, Wang X, McKenzie S, Jain S, Greenhill S, Wilson M, Marley N, Salah A, et al.
Neuron (2023) : . 258 011 ICC, IHC; tested species: mouse,rat

Striatal dopamine D2, adenosine A2A and cannabinoid CB1 receptors balance as a target against non-cognitive symptoms in a mouse model of Alzheimer's disease.
Gómez-Acero L, Sánchez-Fernández N, Subirana P, Ciruela F, Aso E
Pharmacology, biochemistry, and behavior (2025) 249: 173970. 258 011 IHC; tested species: mouse

CB1R blockade unmasks TRPV1-mediated contextual fear generalization in female, but not male rats.
Huckleberry KA, Calitri R, Li AJ, Mejdell M, Singh A, Bhutani V, Laine MA, Nastase AS, Morena M, Hill MN, Shansky RM, et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2023) 4810: 1500-1508. 258 011 IHC; tested species: rat

Dual Cannabinoid and Orexin Regulation of Anhedonic Behaviour Caused by Prolonged Restraint Stress.
Kim HJJ, Zagzoog A, Ceni C, Ferrisi R, Janz N, Laprairie RB
Brain sciences (2023) 132: . 258 011 IHC; tested species: mouse

Social Play Behavior Is Critical for the Development of Prefrontal Inhibitory Synapses and Cognitive Flexibility in Rats.
Bijlsma A, Omrani A, Spoelder M, Verharen JPH, Bauer L, Cornelis C, de Zwart B, van Dorland R, Vanderschuren LJMJ, Wierenga CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4246: 8716-8728. 258 011 IHC; tested species: rat

Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction.
Liu Z, Yang N, Dong J, Tian W, Chang L, Ma J, Guo J, Tan J, Dong A, He K, Zhou J, et al.
Nature communications (2022) 131: 3490. 258 011 IHC; tested species: mouse

Axonal CB1 Receptors Mediate Inhibitory Bouton Formation via cAMP Increase and PKA.
Liang J, Kruijssen DLH, Verschuuren ACJ, Voesenek BJB, Benavides FFW, Sáez Gonzalez M, Ruiter M, Wierenga CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4140: 8279-8296. 258 011 IHC; tested species: mouse

In vivo Evidence for Brain Region-Specific Molecular Interactions Between Cannabinoid and Orexin Receptors.
Kim HJJ, Zagzoog A, Smolyakova AM, Ezeaka UC, Benko MJ, Holt T, Laprairie RB
Frontiers in neuroscience (2021) 15: 790546. 258 011 IHC; tested species: mouse

Effects of ∆9-THC on memory in ovariectomized and intact female rats.
DeLarge AF, Winsauer PJ
Hormones and behavior (2021) 127: 104883. 258 011 IHC; tested species: rat

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 258 011 IHC; tested species: rat

Early-life stress exacerbates the effects of WIN55,212-2 and modulates the cannabinoid receptor type 1 expression.
Martín-Sánchez A, García-Baos A, Castro-Zavala A, Alegre-Zurano L, Valverde O
Neuropharmacology (2020) : 108416. 258 011 IHC; tested species: mouse

Endocannabinoid Signaling Mediates Local Dendritic Coordination between Excitatory and Inhibitory Synapses.
Hu HY, Kruijssen DLH, Frias CP, Rózsa B, Hoogenraad CC, Wierenga CJ
Cell reports (2019) 273: 666-675.e5. 258 011 IHC; tested species: mouse

The cannabinoid-1 receptor is abundantly expressed in striatal striosomes and striosome-dendron bouquets of the substantia nigra.
Davis MI, Crittenden JR, Feng AY, Kupferschmidt DA, Naydenov A, Stella N, Graybiel AM, Lovinger DM
PloS one (2018) 132: e0191436. 258 011 IHC; tested species: mouse

Cat. No.: 258 011

Amount: 100 µg

Price: $425.00

References
WB (1)
ICC (1)
IHC (13)

Effects of ∆9-THC on memory in ovariectomized and intact female rats.
DeLarge AF, Winsauer PJ
Hormones and behavior (2021) 127: 104883. 258 011 IHC; tested species: rat

ICC

IHC

IHC-P

Background

The cannabinoid receptor CB1-R is a G-protein coupled receptor (GPCR) with 7 transmembrane domains. It is responsive to tetrahydrocannabinol, the psychotropic component of marijuana.
Endogenous cannabinoids (endocannabinoids) are released from postsynaptic neurons and act onto presynaptic cannabinoid receptors where they play important physiological roles in synaptic plasticity, analgesia, appetite, and neuroprotection.

1 ) Osborne KD et al., ASN Neuro (2009)
2 ) Niehaus JL et al., Mol. Pharmacol. (2007)
3 ) Kawamura Y et al., J. Neurosci. (2006)
4 ) Shire D et al., J. Biol. Chem. (1995)
5 ) Matsuda LA et al., Nature (1990)
6 ) Howlett AC et al., Mol. Pharmacol. (1985)

Protocols

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

Other products for this target

Cannabinoid receptor CB1-R antibody - 258 011 K.O.

References for Cannabinoid receptor CB1-R - 258 011