Cre-Recombinase antibody - 257 117

Cre-recombinase is used for conditional or tissue specific knockouts

Rat monoclonal purified IgG

Cat. No.: 257 117

Amount: 100 µg

Price: $420.00

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Cat. No. 257 117	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P (FFPE): not recommended (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-303D10
Subtype	IgG2b (κ light chain)
Immunogen	Full length recombinant Cre-recombinase from Bacteriophage P1 (UniProt Id: P06956)
Specificity	Recognizes Cre- and iCre-recombinase.
Remarks	IHC: Antigen retrieval with citrate buffer pH 6 is not advised. IHC-P (FFPE): Cat. no. 257 017 is recommended for IHC-P.
Data sheet	Data Sheet 257_117 Safety Data Sheet (SDS)

Cat. No.: 257 117

Amount: 100 µg

Price: $420.00

Cre-recombinase staining of a subset of nuclei in the hippocampus of a Nex-Cre mouse

ICC
(1 img)

IHC
(4 img)

Background

Cre-Recombinase is a site-specific DNA recombinase derived from bacteriophage P1. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA and catalyzes site-specific recombination between two 34-base-pair LOXP recognition sites, enabling precise deletion, inversion, or activation of DNA sequences. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state [1].
In experimental biology, Cre is commonly expressed under tissue-, cell-, or temporally specific promoters, allowing conditional manipulation of genes in transgenic and knockout mouse models [2, 3]. Inducible variants such as Cre-ERT are fused to a modified estrogen receptor domain and can be activated by tamoxifen, providing temporal control of recombination [4].

1 ) Lanza AM et al., Biotechnol J (2012)
2 ) Nagy A et al., Genesis (2000)
3 ) Brault V et al., Handb Exp Pharmacol (2007)
4 ) Feil R et al., Proc Natl Acad Sci U S A (1996)

Protocols

ICC

Fixed Cells

IHC

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