Cre-Recombinase antibody - 257 017

Cre-recombinase is used for conditional or tissue specific knockouts

Rat monoclonal purified IgG

Cat. No.: 257 017

Amount: 100 µg

Price: $420.00

Cat. No. 257 017	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 100 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-271B7
Subtype	IgG2b (κ light chain)
Immunogen	Full length recombinant Cre-recombinase from Bacteriophage P1 (UniProt Id: P06956)
Specificity	Recognizes Cre- and iCre-recombinase.
Remarks	IHC: Antigen retrieval with citrate buffer pH 6 is tolerated. This antibody is less sensitive compared to cat. no. 257 117, which is the first choice for IHC-experiments.
Data sheet	257_017.pdf

Cat. No.: 257 017

Amount: 100 µg

Price: $420.00

Cre-recombinase staining of a subset of nuclei in the intestine of a Villin-Cre mouse

ICC

IHC

IHC-P

Background

Cre-Recombinase is a 38 kDa type 1 topoisomerase from bacteriophage P1. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA and catalyzes site-specific recombination between two 34-base-pair LOXP recognition sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. Its highly specific catalytic activity makes it a valuable tool for the generation of conditional or tissue specific mutants.

1 ) Ferguson BW et al., PLoS ONE (2012)
2 ) Lanza AM et al., Biotechnol J (2012)
3 ) Smedley D et al., Methods (2011)
4 ) Brault V et al., Handb Exp Pharmacol (2007)
5 ) Abremski K et al., J. Biol. Chem. (1984)
6 ) Hoess R et al., Cold Spring Harb. Symp. Quant. Biol. (1984)

Protocols

ICC

IHC

IHC-P

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